Human Calponin-2(CNN2) ELISA kit

Code CSB-EL005656HU
Size 96T,5×96T,10×96T
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Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
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Product Details

Target Name
calponin 2
Alternative Names
Calponin 2 ELISA Kit; Calponin H2 ELISA Kit; Calponin H2, smooth muscle ELISA Kit; Calponin-2 ELISA Kit; Cnn2 ELISA Kit; CNN2_HUMAN ELISA Kit; Neutral calponin ELISA Kit; smooth muscle ELISA Kit
Abbreviation
CNN2
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
31.25 pg/mL-2000 pg/mL
Sensitivity
7.8 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CNN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-89  
1:2 Average % 98  
Range % 93-103  
1:4 Average % 95  
Range % 89-98  
1:8 Average % 97  
Range % 92-101  
Recovery
The recovery of human CNN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 98-106  
EDTA plasma (n=4) 94 86-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.325 2.413 2.369 2.254  
1000 1.876 1.941 1.909 1.794  
500 1.374 1.282 1.328 1.213  
250 0.752 0.764 0.758 0.643  
125 0.434 0.441 0.438 0.323  
62.5 0.282 0.297 0.290 0.175  
31.25 0.183 0.187 0.185 0.070  
0 0.114 0.115 0.115    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CNN2 ELISA Kit was designed for the quantitative measurement of Human CNN2 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.

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Target Background

Function
(From Uniprot)
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
Gene References into Functions
  1. Study in human hepatocellular carcinoma cell line and xenograft mouse model shows that CNN2 plays an important role in tumor growth and metastasis, possibly through MEK1/2-ERK1/2 signaling pathway. PMID: 29333089
  2. our results demonstrated that knockdown of endogenous CNN2 in AGS cells could significantly activate cell apoptosis pathway and therefore suppress cell growth in vitro. The deletion of CNN2 might be a potential therapeutic approach to inhibit aggressive growth of gastric cancer. PMID: 28714360
  3. High Calponin-h2 serum levels are associated with breast cancer. PMID: 25099617
  4. h2-Calponin gene expression is further affected by the treatment of cells with Notch inhibitor and activator, suggesting an upstream signaling mechanism. PMID: 24285540
Protein Families
Calponin family
Tissue Specificity
Heart and smooth muscle.
Database Links

HGNC: 2156

OMIM: 602373

KEGG: hsa:1265

STRING: 9606.ENSP00000263097

UniGene: Hs.651512

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