Recombinant Human Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2 (CTDSP2)

Code CSB-YP006143HU
MSDS
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Source Yeast
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Code CSB-EP006143HU
MSDS
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Source E.coli
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Code CSB-EP006143HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP006143HU
MSDS
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Source Baculovirus
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Code CSB-MP006143HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CTDSP2
Uniprot No.
Alternative Names
Carboxy terminal domain RNA polymerase II polypeptide A small phosphatase 2; Carboxy-terminal domain RNA polymerase II polypeptide A small phosphatase 2; conserved gene amplified in osteosarcoma 4; conserved gene amplified in osteosarcoma; CTDS2_HUMAN; Ctdsp2; NIF2; NLI interacting factor 2; NLI-interacting factor 2; Nuclear LIM interactor interacting factor 2; Nuclear LIM interactor-interacting factor 2; OS4; Protein OS 4; Protein OS-4; PSR2; SCP2; Small C-terminal domain phosphatase 2; Small CTD phosphatase 2
Species
Homo sapiens (Human)
Expression Region
1-271
Target Protein Sequence
MEHGSIITQA RREDALVLTK QGLVSKSSPK KPRGRNIFKA LFCCFRAQHV GQSSSSTELA AYKEEANTIA KSDLLQCLQY QFYQIPGTCL LPEVTEEDQG RICVVIDLDE TLVHSSFKPI NNADFIVPIE IEGTTHQVYV LKRPYVDEFL RRMGELFECV LFTASLAKYA DPVTDLLDRC GVFRARLFRE SCVFHQGCYV KDLSRLGRDL RKTLILDNSP ASYIFHPENA VPVQSWFDDM ADTELLNLIP IFEELSGAED VYTSLGQLRA P
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Preferentially catalyzes the dephosphorylation of 'Ser-5' within the tandem 7 residue repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Recruited by REST to neuronal genes that contain RE-1 elements, leading to neuronal gene silencing in non-neuronal cells. May contribute to the development of sarcomas.
Gene References into Functions
  1. CTDSP2 induces p21(Cip1/Waf1) through increasing the activity of Ras. PMID: 25990325
  2. These results suggest that SCP2 is involved in promoter clearance during steroid-activated transcription. PMID: 16724108
  3. SCP2 acts as a phosphatase for regulatory phosphorylations in the linker region of Smad1 and Smad2 PMID: 17085434
Subcellular Location
Nucleus.
Tissue Specificity
Expression is restricted to non-neuronal tissues. Highest expression in pancreas and lowest in liver.
Database Links

HGNC: 17077

OMIM: 608711

KEGG: hsa:10106

STRING: 9606.ENSP00000381148

UniGene: Hs.524530

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