Recombinant Human Carboxypeptidase O (CPO)

Code CSB-YP005909HU
MSDS
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Source Yeast
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Code CSB-EP005909HU
MSDS
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Source E.coli
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Code CSB-EP005909HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005909HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005909HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CPO
Uniprot No.
Alternative Names
Carboxypeptidase O; CBPO_HUMAN; CPO; Metallocarboxypeptidase C; Metallocarboxypeptidase O; MGC138281; MGC138283
Species
Homo sapiens (Human)
Expression Region
21-374
Target Protein Sequence
YDRSLAQHRQ EIVDKSVSPW SLETYSYNIY HPMGEIYEWM REISEKYKEV VTQHFLGVTY ETHPMYYLKI SQPSGNPKKI IWMDCGIHAR EWIAPAFCQW FVKEILQNHK DNSSIRKLLR NLDFYVLPVL NIDGYIYTWT TDRLWRKSRS PHNNGTCFGT DLNRNFNASW CSIGASRNCQ DQTFCGTGPV SEPETKAVAS FIESKKDDIL CFLTMHSYGQ LILTPYGYTK NKSSNHPEMI QVGQKAANAL KAKYGTNYRV GSSADILYAS SGSSRDWARD IGIPFSYTFE LRDSGTYGFV LPEAQIQPTC EETMEAVLSV LDDVYAKHWH SDSAGRVTSA TMLLGLLVSC MSLL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Carboxypeptidase which preferentially cleaves C-terminal acidic residues from peptides and proteins. Can also cleave C-terminal hydrophobic amino acids, with a preference for small residues over large residues.
Gene References into Functions
  1. hCPO can also trim C-t extensions of proteins, such as epidermal growth factor, suggesting a role in the maturation and degradation of growth factors and bioactive peptides. PMID: 29636417
  2. CPO cleaves acidic amino acids from dietary proteins and peptides, thus complementing the actions of well known digestive carboxypeptidases CPA and CPB. PMID: 21921028
Subcellular Location
Apical cell membrane; Lipid-anchor, GPI-anchor.
Protein Families
Peptidase M14 family
Tissue Specificity
Detected in enterocytes of the ileum.
Database Links

HGNC: 21011

OMIM: 609563

KEGG: hsa:130749

STRING: 9606.ENSP00000272852

UniGene: Hs.684103

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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