Recombinant Human Cell cycle checkpoint protein RAD17 (RAD17)

1. DDX11 orchestrates jointly with 9-1-1 and its loader, RAD17, DNA damage tolerance at sites of bulky lesions, and endogenous abasic sites. These functions may explain the essential roles of DDX11 and its similarity with 9-1-1 during development. PMID: 30061412
2. The Rad17 C-terminal tail is a molecular switch that regulates the 9-1-1 interaction and the ATR pathway. PMID: 28666868
3. These data indicate that the interaction with the 9-1-1 complex is not required for Rad17 protein to be an efficient substrate for the UV-induced phosphorylation. Our data also raise the possibility that the 9-1-1 complex plays a negative regulatory role in the Rad17 phosphorylation. We also show that the nucleotide-binding activity of Rad17 is required for its nuclear localization. PMID: 27387238
4. In a Japanese population, the variant allele of hRAD17 is significantly associated with a decreased risk of Colorectal Cancer among light smokers and rectal cancer patients and with an increased risk of Colorectal Cancer among heavy smokers. PMID: 28238011
5. Authors show that BRCA1 and RAD17 genes, whose derived proteins play a pivotal role in DNA damage repair, are transcriptional targets of gain-of-function mutant p53 proteins. PMID: 25650659
6. USP20 and Rad17 interact, and that this interaction is enhanced by UV exposure. We show that USP20 regulation of Rad17 is at the protein level in a proteasome-dependent manner. USP20 depletion results in poor activation of Chk1 protein by phosphorylation PMID: 24923443
7. Rad17 is phosphorylated by ATM at Thr622 resulting in a direct interaction of Rad17 with NBS1, facilitating recruitment of MRE11, RAD50 and ATM to the DNA double-strand breaks. PMID: 24534091
8. Our data suggest RAD17 as a novel target protein for gemcitabine combination therapy supplementing or complementing inhibition of CHK1. PMID: 23687379
9. Knockdown of Rad17 with two independent siRNAs significantly reduced Chk1 phosphorylation and substantial RPA32 Ser33 phosphorylation. PMID: 23684611
10. Data indicate that regulation of Rad17 turnover is through the Cdh1/anaphase-promoting complex pathway in breast cancer cells. PMID: 23637229
11. Proteolysis of Rad17 by Cdh1/APC regulates checkpoint termination and recovery from genotoxic stress PMID: 20424596
12. The four alternatively spliced forms differentially expressed in different tissues, in different phases of the cell cycle, and differentially responded to X-irradiation. PMID: 11602352
13. upon replication block a Rad17/RF-C complex is recruited to sites of DNA lesions in late S phase, binds the Rad9/Hus1/Rad1 complex and enables it to interact with PCNA. An interaction of Rad17/RF-C with PCNA is mediated by the small RF-C p37 subunit PMID: 12400013
14. Rad17 localizes to DNA replication sites and interacts with DNA polymerase epsilon. PMID: 14500819
15. replication protein A (RPA) stimulates the binding of the Rad17-Rfc2-5 complex to single-stranded DNA PMID: 14605214
16. we show a requirement for Rad17 and Hus1 to induce G(2) arrest as well as Vpr-induced phosphorylation of histone 2A variant X (H2AX) and formation of nuclear foci containing H2AX and breast cancer susceptibility protein 1 PMID: 15485898
17. interacts with newly identified hMCM7 protein, a core component of the DNA replication apparatus PMID: 15538388
18. Findings reveal a phosphorylation-dependent function of Rad17 in an ATR-Rad17-Claspin-Chk1-signaling cascade that responds to specific replication stress. PMID: 16885023
19. Using siRNA to knock down Rad17 demonstrates that it is not essntial for the DNA replication checkpoint in Hela cells. PMID: 16951182
20. Loss of hRAD17 expression occurs frequently in HNSCC, is often due to genomic deletion, and may facilitate genomic instability in HNSCC PMID: 17657792
21. hRAD17 delayed growth of NIH3T3 fibroblasts transformed by the H-ras oncogene in nude mice. PMID: 18378394

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HGNC: 9807

OMIM: 603139

KEGG: hsa:5884

STRING: 9606.ENSP00000370151

UniGene: Hs.16184