Recombinant Human Cell cycle checkpoint protein RAD1(RAD1)

Code CSB-EP019252HU
Product Type Recombinant Protein
Size US$1726
Uniprot No. O60671
Relevance Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Isoform 1 possesses 3'->5' double stranded DNA exonuclease activity.
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Storage Buffer Tris-based buffer,50% glycerol
Alias DNA repair exonuclease rad1 homolog Rad1-like DNA damage checkpoint protein
Species Homo sapiens (Human)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence MPLLTQQIQDEDDQYSLVASLDNVRNLSTILKAIHFREHATCFATKNGIKVTVENAKCVQANAFIQAGIFQEFKVQEESVTFRINLTVLLDCLSIFGSSPMPGTLTALRMCYQGYGYPLMLFLEEGGVVTVCKINTQEPEETLDFDFCSTNVINKIILQSEGLREAFSELDMTSEVLQITMSPDKPYFRLSTFGNAGSSHLDYPKDSDLMEAFHCNQTQVNRYKISLLKPSTKALVLSCKVSIRTDNRGFLSLQYMIRNEDGQICFVEYYCCPDEEVPESES
Research Area Epigenetics and Nuclear Signaling
Source E.coli
Gene Names RAD1
Protein Names Recommended name: Cell cycle checkpoint protein RAD1 Short name= hRAD1 EC= 3.1.11.2 Alternative name(s): DNA repair exonuclease rad1 homolog Rad1-like DNA damage checkpoint protein
Expression Region 1-282aa
Tag Info N-terminal GST-tagged
Mol. Weight 58.8kDa
Protein Description Full Length
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair
Subcellular Location Nucleus
Protein Families Rad1 family
Tissue Specificity Expressed in testis, uterus, bladder, spleen, ovaries, lung, brain and muscle (at protein level).
Database Links

HGNC: 9806

OMIM: 603153

KEGG: hsa:5810

STRING: 9606.ENSP00000340879

UniGene: Hs.38114

Pathway Cellular senescence

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