Recombinant Human Centromere protein V (CENPV)

Code CSB-YP005222HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005222HU
MSDS
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Source E.coli
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Code CSB-EP005222HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005222HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005222HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CENPV
Uniprot No.
Alternative Names
3110013H01Rik; CENP V; CENP-V; Cenpv; CENPV_HUMAN; Centromere protein V; Nuclear protein p30; p30; Proline rich 6; Proline rich protein 6; Proline-rich protein 6; PRR6
Species
Homo sapiens (Human)
Expression Region
1-275
Target Protein Sequence
MRRSRSSAAA KLRGQKRSGA SGASAAPAAS AAAALAPSAT RTRRSASQAG SKSQAVEKPP SEKPRLRRSS PRAQEEGPGE PPPPELALLP PPPPPPPTPA TPTSSASNLD LGEQRERWET FQKRQKLTSE GAAKLLLDTF EYQGLVKHTG GCHCGAVRFE VWASADLHIF DCNCSICKKK QNRHFIVPAS RFKLLKGAEH ITTYTFNTHK AQHTFCKRCG VQSFYTPRSN PGGFGIAPHC LDEGTVRSMV TEEFNGSDWE KAMKEHKTIK NMSKE
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for distribution of pericentromeric heterochromatin in interphase nuclei and for centromere formation and organization, chromosome alignment and cytokinesis.
Gene References into Functions
  1. Results suggest that CENP-V (nuclear protein p30) is required for centromere organization, chromosome alignment and cytokinesis in human cells PMID: 18772885
Subcellular Location
Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Enriched at the nuclear periphery and around the nucleolus (PubMed:12196509). In mitotic cells, localizes to kinetochores from prometaphase to metaphase (PubMed:18772885). At anaphase onset, transfers to the spindle midzone and then to the mid-body in telophase and cytokinesis (PubMed:18772885).
Protein Families
Gfa family
Database Links

HGNC: 29920

OMIM: 608139

KEGG: hsa:201161

STRING: 9606.ENSP00000299736

UniGene: Hs.433422

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