Recombinant Human Centrosomal protein of 89 kDa (CEP89)

Code CSB-YP853505HU
MSDS
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Source Yeast
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Code CSB-EP853505HU
MSDS
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Source E.coli
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Code CSB-EP853505HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853505HU
MSDS
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Source Baculovirus
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Code CSB-MP853505HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CEP89
Uniprot No.
Alternative Names
CCDC123; Centrosomal protein 89 kDa; Centrosomal protein of 89 kDa; cep89; CEP89_HUMAN; Coiled-coil domain-containing protein 123; Coiled-coil domain-containing protein 123; mitochondrial; FLJ14640
Species
Homo sapiens (Human)
Expression Region
1-783
Target Protein Sequence
MLLGFRRGRR SHFKHIIHGL LPAASVAPKA AVPRTPPPRS PNPSPERPRS ALAAAILATT LTGRTVAIPQ PRQRSRSESD VSSVEQDSFI EPYATTSQLR PRPNWQSEMG RRSSLPSFET LDYGDEEDIE TQLSSSGKEL GDVSAREDRG GHSDDLYAVP HRNQVPLLHE VNSEDDENIS HQDGFPGSPP APQRTQQKDG KHPVLNLKDE KPPLCEKPPP SPDITGRARQ RYTEITREKF EALKEENMDL NNMNQSLTLE LNTMKQAMKE LQLKLKGMEK EKRKLKEAEK ASSQEVAAPE LLYLRKQAQE LVDENDGLKM TVHRLNVELS RYQTKFRHLS KEESLNIEGL PSKGPIPPWL LDIKYLSPLL LAYEDMMKEK DELNATLKEE MRMFRMRVQE VVKENEELHQ ELNKSSAVTS EEWRQLQTQA KLVLEENKLL LEQLEIQQRK AKDSHQERLQ EVSKLTKQLM LLEAKTHGQE KELAENREQL EILRAKCQEL KTHSDGKIAV EVHKSIVNEL KSQLQKEEEK ERAEMEELME KLTVLQAQKK SLLLEKNSLT EQNKALEAEL ERAQKINRKS QKKIEVLKKQ VEKAMGNEMS AHQYLANLVG LAENITQERD SLMCLAKCLE SEKDGVLNKV IKSNIRLGKL EEKVKGYKKQ AALKLGDISH RLLEQQEDFA GKTAQYRQEM RHLHQVLKDK QEVLDQALQQ NREMEGELEV IWESTFRENR RIRELLQDTL TRTGVQDNPR ALVAPSLNGV SQADLLDGCD VCSYDLKSHA PTC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for ciliogenesis. Also plays a role in mitochondrial metabolism where it may modulate complex IV activity.
Gene References into Functions
  1. CEP89 proteins play an important role in mitochondrial metabolism, especially complex IV activity, and are required for neuronal and cognitive function across evolution. PMID: 23575228
Involvement in disease
Homozygous deletion comprising CEP89 and SLC7A9 genes has been reported in a patient with isolated complex IV deficiency, intellectual disability and multisystemic problems that include cystinuria, cataract, broad based walking pattern and deafness.
Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Mitochondrion intermembrane space. Note=Localizes to the distal appendage region of the centriole, which anchors the mother centriole to the plasma membrane.
Database Links

HGNC: 25907

OMIM: 615470

KEGG: hsa:84902

STRING: 9606.ENSP00000306105

UniGene: Hs.599703

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