Recombinant Human Citrate lyase subunit beta-like protein, mitochondrial (CLYBL)

Code CSB-YP850772HU
MSDS
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Source Yeast
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Code CSB-EP850772HU
MSDS
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Source E.coli
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Code CSB-EP850772HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP850772HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP850772HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLYBL
Uniprot No.
Alternative Names
CLYBL; CLB; Citramalyl-CoA lyase; mitochondrial; EC 4.1.3.25;; 3S)-malyl-CoA thioesterase; EC 3.1.2.30; Beta-methylmalate synthase; EC 2.3.3.-; Citrate lyase subunit beta-like protein; Citrate lyase beta-like; Malate synthase; EC 2.3.3.9
Species
Homo sapiens (Human)
Expression Region
23-340
Target Protein Sequence
ASLAADIP RLGYSSSSHH KYIPRRAVLY VPGNDEKKIK KIPSLNVDCA VLDCEDGVAA NKKNEARLRI VKTLEDIDLG PTEKCVRVNS VSSGLAEEDL ETLLQSRVLP SSLMLPKVES PEEIQWFADK FSFHLKGRKL EQPMNLIPFV ETAMGLLNFK AVCEETLKVG PQVGLFLDAV VFGGEDFRAS IGATSSKETL DILYARQKIV VIAKAFGLQA IDLVYIDFRD GAGLLRQSRE GAAMGFTGKQ VIHPNQIAVV QEQFSPSPEK IKWAEELIAA FKEHQQLGKG AFTFQGSMID MPLLKQAQNT VTLATSIKEK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism. Converts citramalyl-CoA into acetyl-CoA and pyruvate in the C5-dicarboxylate catabolism pathway. The C5-dicarboxylate catabolism pathway is required to detoxify itaconate, a vitamin B12-poisoning metabolite. Also acts as a malate synthase in vitro, converting glyoxylate and acetyl-CoA to malate. Also displays malyl-CoA thioesterase activity. Also acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. Also has very weak citramalate synthase activity in vitro.
Gene References into Functions
  1. Study reports that CLYBL loss leads to a cell-autonomous defect in the mitochondrial B12 metabolism and that itaconyl-CoA is a cofactor-inactivating, substrate-analog inhibitor of the mitochondrial B12-dependent methylmalonyl-CoA mutase PMID: 29056341
Subcellular Location
Mitochondrion.
Protein Families
HpcH/HpaI aldolase family, Citrate lyase beta subunit-like subfamily
Database Links

HGNC: 18355

OMIM: 609686

KEGG: hsa:171425

STRING: 9606.ENSP00000342991

UniGene: Hs.655642

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