Recombinant Human Coatomer subunit epsilon (COPE)

Code CSB-YP005785HU
MSDS
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Source Yeast
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Code CSB-EP005785HU
MSDS
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Source E.coli
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Code CSB-EP005785HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005785HU
MSDS
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Source Baculovirus
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Code CSB-MP005785HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COPE
Uniprot No.
Alternative Names
1110005D17Rik; Coatomer epsilon subunit; Coatomer protein complex subunit epsilon; Coatomer subunit epsilon; COPE; COPE_HUMAN; Cope1; Epsilon coat protein; Epsilon COP; Epsilon COP I; Epsilon subunit of coatomer protein complex; Epsilon-coat protein; Epsilon-COP; FLJ13241
Species
Homo sapiens (Human)
Expression Region
2-308
Target Protein Sequence
APPAPGPAS GGSGEVDELF DVKNAFYIGS YQQCINEAQR VKLSSPERDV ERDVFLYRAY LAQRKFGVVL DEIKPSSAPE LQAVRMFADY LAHESRRDSI VAELDREMSR SVDVTNTTFL LMAASIYLHD QNPDAALRAL HQGDSLECTA MTVQILLKLD RLDLARKELK RMQDLDEDAT LTQLATAWVS LATGGEKLQD AYYIFQEMAD KCSPTLLLLN GQAACHMAQG RWEAAEGLLQ EALDKDSGYP ETLVNLIVLS QHLGKPPEVT NRYLSQLKDA HRSHPFIKEY QAKENDFDRL VLQYAPSA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. The coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors.
Gene References into Functions
  1. Ubiquitylation of epsilon-COP by PIRH2; overexpression of the wild type of PIRH2 in prostate cancer cells causes downregulation of the secretion of prostate-specific antigen. PMID: 17721809
  2. Data show that iPLA(1)gamma is a novel membrane transport factor that contributes to a specific Golgi-to-ER retrograde pathway distinct from presently characterized COPI- and Rab6-dependent pathways. PMID: 19632984
Subcellular Location
Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle, COPI-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
COPE family
Database Links

HGNC: 2234

OMIM: 606942

KEGG: hsa:11316

STRING: 9606.ENSP00000262812

UniGene: Hs.10326

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