Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

COG5

Uniprot No.

Q9UP83

Research Area

Signal Transduction

Alternative Names

(COG complex subunit 5)(13S Golgi transport complex 90 kDa subunit)(GTC-90)(Component of oligomeric Golgi complex 5)(Golgi transport complex 1)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-257aa

Target Protein Sequence

MGWVGGRRRDSASPPGRSRSAADDINPAPANMEGGGGSVAVAGLGARGSGAAAATVRELLQDGCYSDFLNEDFDVKTYTSQSIHQAVIAEQLAKLAQGISQLDRELHLQVVARHEDLLAQATGIESLEGVLQMMQTRIGALQGAVDRIKAKIVEPYNKIVARTAQLARLQVACDLLRRIIRILNLSKRLQGQLQGGSREITKAAQSLNELDYLSQGIDLSGIEVIENDLLFIARARLEVENQAKRLLEQGLETQNPT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

33.8 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human COG5 was expressed with the amino acid range of 1-257. The theoretical molecular weight of the COG5 protein is 33.8 kDa. This COG5 protein is produced using e.coli expression system. The COG5 coding gene included the N-terminal 10xHis tag, which simplifies the detection and purification processes of the recombinant COG5 protein in following stages of expression and purification.

Conserved oligomeric Golgi complex subunit 5 (COG5) is a part of the COG complex, which is involved in maintaining the structure and function of the Golgi apparatus. COG5 specifically plays a role in tethering vesicles and facilitating the intra-Golgi transport of proteins. Mutations in the COG5 gene can lead to defects in protein glycosylation and result in congenital disorders of glycosylation (CDG), affecting various physiological processes. Research areas involving COG5 include investigating its role in intracellular trafficking, glycosylation disorders, and cellular homeostasis. Understanding COG5's functions contributes to insights into fundamental cellular processes and potential therapeutic targets for related disorders.

Target Background

Function

Required for normal Golgi function.

Gene References into Functions

Targeted silencing of components of lobe B of the COG complex, namely COG5, COG6, COG7 and COG8, inhibited HIV-1 replication PMID: 25179963
Cog5-Cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex. PMID: 25331899
In conclusion, we find that GDF5, but not COG5 or MCF2L, influence the extent of radiographic damage in knee osteoarthritis PMID: 22615457
Report on a uterine leiomyoma with a novel HMGA2 fusion gene in a 44-year-old woman. PMID: 20804914
Sec34 is implicated in traffic from the endoplasmic reticulum to the Golgi and exists in a complex with GTC-90 and ldlBp PMID: 11929878
COG5- and COG7 subunits play distinctive roles in controlling Golgi structure and function PMID: 16051600

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Involvement in disease

Congenital disorder of glycosylation 2I (CDG2I)

Subcellular Location

Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein.

Protein Families

COG5 family

Database Links

HGNC: 14857

OMIM: 606821

KEGG: hsa:10466

STRING: 9606.ENSP00000297135

UniGene: Hs.239631

Code	CSB-EP890771HU
Abbreviation	Recombinant Human COG5 protein, partial
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Conserved oligomeric Golgi complex subunit 5 (COG5), partial

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