Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Signal Transduction
Alternative Names
(COG complex subunit 5)(13S Golgi transport complex 90 kDa subunit)(GTC-90)(Component of oligomeric Golgi complex 5)(Golgi transport complex 1)
Species
Homo sapiens (Human)
Expression Region
1-257aa
Target Protein Sequence
MGWVGGRRRDSASPPGRSRSAADDINPAPANMEGGGGSVAVAGLGARGSGAAAATVRELLQDGCYSDFLNEDFDVKTYTSQSIHQAVIAEQLAKLAQGISQLDRELHLQVVARHEDLLAQATGIESLEGVLQMMQTRIGALQGAVDRIKAKIVEPYNKIVARTAQLARLQVACDLLRRIIRILNLSKRLQGQLQGGSREITKAAQSLNELDYLSQGIDLSGIEVIENDLLFIARARLEVENQAKRLLEQGLETQNPT
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Human COG5 was expressed with the amino acid range of 1-257. The theoretical molecular weight of the COG5 protein is 33.8 kDa. This COG5 protein is produced using e.coli expression system. The COG5 coding gene included the N-terminal 10xHis tag, which simplifies the detection and purification processes of the recombinant COG5 protein in following stages of expression and purification.
Conserved oligomeric Golgi complex subunit 5 (COG5) is a part of the COG complex, which is involved in maintaining the structure and function of the Golgi apparatus. COG5 specifically plays a role in tethering vesicles and facilitating the intra-Golgi transport of proteins. Mutations in the COG5 gene can lead to defects in protein glycosylation and result in congenital disorders of glycosylation (CDG), affecting various physiological processes. Research areas involving COG5 include investigating its role in intracellular trafficking, glycosylation disorders, and cellular homeostasis. Understanding COG5's functions contributes to insights into fundamental cellular processes and potential therapeutic targets for related disorders.
