Recombinant Human Cytochrome c oxidase copper chaperone (COX17)

Code CSB-YP619858HU
MSDS
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Source Yeast
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Code CSB-EP619858HU
MSDS
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Source E.coli
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Code CSB-EP619858HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619858HU
MSDS
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Source Baculovirus
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Code CSB-MP619858HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COX17
Uniprot No.
Alternative Names
COX 17; COX17; COX17 cytochrome c oxidase assembly homolog (S. cerevisiae); COX17 cytochrome c oxidase assembly homolog; COX17 homolog cytochrome c oxidase assembly protein; COX17_HUMAN; cytochrome c oxidase assembly protein cox17 homolog; Cytochrome c oxidase copper chaperone; Human homolog of yeast mitochondrial copper recruitment; MGC104397; MGC117386; OTTHUMP00000215284; OTTHUMP00000215285
Species
Homo sapiens (Human)
Expression Region
1-63
Target Protein Sequence
MPGLVDSNPA PPESQEKKPL KPCCACPETK KARDACIIEK GEEHCGHLIE AHKECMRALG FKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).
Gene References into Functions
  1. These results collectively indicate that Cox17 might not participate in the action of these anticancer organoruthenium complexes, and further verify the distinct anticancer mechanism of the organoruthenium(II) complexes from cisplatin. PMID: 27235272
  2. Data show that the redox state of cytochrome c oxidase assembly protein 17 (Cox17), mitochondrial membrane transport protein Mia40 and superoxide dismutase 1 (SOD1) in the cytoplasm were directly observed with in-cell NMR spectroscopy. PMID: 26589182
  3. Data suggest that Cox17 enhances the reactivity of some platinum/organoplatinum antineoplastic drugs but suppresses the reactivity of at least one platinum antineoplastic drug; glutathione modulates binding of platinum/organoplatinum drugs to Cox17. PMID: 26399480
  4. Functional role of two interhelical disulfide bonds in human Cox17 protein from a structural perspective. PMID: 21816817
  5. Data imply that up-regulation of COX17 function and increased cytochrome c oxidase activity are frequent features of lung carcinogenesis. PMID: 14612491
  6. The binding of copper to Cox17 is needed to activate cytochrome c oxidase by Cox17. PMID: 15504366
  7. Cox17-mediated copper metallation of Sco1, as well as the subsequent failure of Cu(A) site maturation, is the basis for the inefficient assembly of the cytochrome c oxidase complex in SCO1 patients PMID: 16520371
  8. Cox17 was studied with regard to its expression, purification, and formation of mixed disulfide adducts with sulfhydryl reagents. PMID: 17208454
  9. XAS (X-ray absorption spectroscopy) determined that Cu4Cox17 contains a Cu4S6-type copper-thiolate cluster, which may provide safe storage of an excess of copper ions PMID: 17672825
  10. Cu(I)HCox17(2S-S), i.e., the copper-loaded form of the protein, can transfer simultaneously copper(I) and two electrons to the human cochaperone Sco1 (HSco1) in the oxidized state, i.e., with its metal-binding cysteines forming a disulfide bond. PMID: 18458339
  11. the absence of Cox17 interferes with copper delivery to Cox2, but not to Cox1. PMID: 19393246

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Subcellular Location
Mitochondrion intermembrane space. Cytoplasm.
Protein Families
COX17 family
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 2264

OMIM: 604813

KEGG: hsa:10063

STRING: 9606.ENSP00000261070

UniGene: Hs.534383

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