Recombinant Human Cytokine-inducible SH2-containing protein(CISH)

Code CSB-EP885721HU
Size US$1726
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CISH
Uniprot No. Q9NSE2
Research Area Signal Transduction
Alternative Names BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-258aa
Target Protein Sequence MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 55.7kDa
Protein Length Full Length
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity).
Gene References into Functions
  1. identified P2RX2, KCNQ5, ERBB3 and SOCS3 to be associated with the progression of age-related hearing impairment PMID: 29325454
  2. CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
  3. Studies indicate that suppressors of cytokine signaling (SOCS) proteins CIS, SOCS1, and SOCS3 can be considered the third immunocheckpoint molecules since they regulate cytokine signals that control the polarization of CD4(+) T cells and the maturation of CD8(+) T cells. PMID: 28188673
  4. CISH rs414171 and rs6768300 variants might be associated with protection from pulmonary tuberculosis in Zahedan, Southeast Iran. PMID: 27266592
  5. This study showed that there was significantly increased levels of CIS mRNA in elderly and Alzheimer's disease brains. PMID: 25286386
  6. Data indicate that CIS protein stability is regulated through multiple mechanisms, including ubiquitination and interaction with Elongin B/C proteins, whereas CIS functional inhibition of PRLR signaling is dependent on the Elongin B/C interaction. PMID: 25448846
  7. SOCS single nucleotide polymorphism is associated with breast cancer. PMID: 25104439
  8. Study in Chinese Han population confirms that previously identified variants of CISH are associated with susceptibility to pulmonary tuberculosis. PMID: 25460819
  9. CISH gene polymorphisms at -292 (rs414171) are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase, and AA is a favorable genotype for this effect. PMID: 24791876
  10. Two SNPs (rs414171 and rs2239751) in the CISH gene were associated with persistent HBV infection in Han Chinese population PMID: 24964072
  11. two genetic variants in CISH gene appear to increase susceptibility to TB in Chinese Han population PMID: 23949851
  12. CISH promoter rs414171 and rs809451 polymorphisms may play a vital role in mediating individual susceptibility to tuberculosis PMID: 24632804
  13. methylation of SOCS1, SOCS2, SOCS3 and CISH is infrequent in Ph-ve MPN. PMID: 24131863
  14. data suggest FXR-mediated upregulation of cytokine inducible SH2-containing protein (CISH) may play an important role in the homeostasis of cytokine signal networks and be beneficial to control cytokine-associated inflammatory diseases PMID: 22817871
  15. In GH transduction defect, abnormal GH signalling may be caused by over-expression of CIS, which may increase degradation of GHR PMID: 23426819
  16. Suppressors of both cytokine signaling SOCS7 and cytokine-inducible SH2 protein (CIS) are expressed constitutively at higher levels in LNCaP-S17 (prostate cancer) cells than in LNCaP-C3 cells. PMID: 22213096
  17. Study indicates the vital role of CISH single nucleotide polymorphism (SNP)-292A>T variant to hepatitis B virus infection in a Vietnamese population. PMID: 22033525
  18. The authors conclude that Mycobacterium tuberculosis infection induces development of Tregs from CCR4(+) cells through a process that depends on PD-1and CISH. PMID: 21383382
  19. Variants of CISH are associated with susceptibility to diseases caused by diverse infectious pathogens. PMID: 20484391
  20. Low level of CISH expression in various tissues suggests that it is a novel prospective marker for diagnosis of colon cancer. PMID: 19807022
  21. Up-regulation of cytokine inducible SH2-containing protein is associated with adult T-cell leukemia PMID: 14630083
  22. CIS (cytokine-inducible SH2 protein)is binding with the conserved Y985 and Y1077 motifs in the cytosolic domain of the leptin receptor. PMID: 16684815
  23. analysis of STAT5, CIS, and SOCS2 interactions with the growth hormone receptor PMID: 17666591
  24. RACK1 has a role in protecting cancer cells from apoptosis by regulating the degradation of BimEL, which together with CIS could play an important role of drug resistance in chemotherapy PMID: 18420585
  25. Elongin B/C recruitment regulates substrate binding by CIS PMID: 18508766
  26. CISH expression in allergen-stimulated CD4(+) T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children. PMID: 18647318
  27. CIS can serve as an SLE disease marker and may be involved in the pathogenesis of SLE, and that TNF-alpha may play an important role in the regulation of CIS PMID: 18820827
  28. miR-98 and let-7 confer cholangiocyte expression of CIS in response to microbial challenge, a process that may be relevant to the regulation of TLR-mediated epithelial innate immune response. PMID: 19592657
  29. These results suggest that RSV infection escapes the innate antiviral response by inducing SOCS1, SOCS3 or CIS expression in epithelial cells. PMID: 19595407

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Tissue Specificity Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.
Database Links

HGNC: 1984

OMIM: 602441

KEGG: hsa:1154

STRING: 9606.ENSP00000409346

UniGene: Hs.655334

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