Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CISH

Uniprot No.

Q9NSE2

Research Area

Signal Transduction

Alternative Names

BACTS2; cis 1; CIS; CIS-1; CIS1; Cish; CISH_HUMAN; Cytokine inducible inhibitor of signaling type 1B; Cytokine inducible SH2 containing protein; Cytokine-inducible SH2-containing protein; G18; Protein G18; SOCS; Suppressor of cytokine signaling

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-258aa

Target Protein Sequence

MVLCVQGPRPLLAVERTGQRPLWAPSLELPKPVMQPLPAGAFLEEVAEGTPAQTESEPKVLDPEEDLLCIAKTFSYLRESGWYWGSITASEARQHLQKMPEGTFLVRDSTHPSYLFTLSVKTTRGPTNVRIEYADSSFRLDSNCLSRPRILAFPDVVSLVQHYVASCTADTRSDSPDPAPTPALPMPKEDAPSDPALPAPPPATAVHLKLVQPFVRRSSARSLQHLCRLVINRLVADVDCLPLPRRMADYLRQYPFQL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

55.7kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Dive into signal transduction research with our premium Recombinant Human CISH protein, a vital component involved in cytokine signaling pathways. The cytokine-inducible SH2-containing protein (CISH) serves as a negative regulator of cytokine signaling, making it an essential player in immune responses and cellular homeostasis.

This full-length Recombinant Human CISH protein is expressed in E. coli and encompasses the 1-258aa expression region. The N-terminal GST-tag enables efficient protein purification and detection, contributing to a dependable product for your research endeavors. Boasting a purity greater than 90% as determined by SDS-PAGE, our Recombinant Human CISH protein ensures reliability and consistency in your signal transduction research. Available in lyophilized powder form, this protein can be conveniently incorporated into various experimental setups.

Target Background

Function

SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. CIS is involved in the negative regulation of cytokines that signal through the JAK-STAT5 pathway such as erythropoietin, prolactin and interleukin 3 (IL3) receptor. Inhibits STAT5 trans-activation by suppressing its tyrosine phosphorylation. May be a substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Gene References into Functions

identified P2RX2, KCNQ5, ERBB3 and SOCS3 to be associated with the progression of age-related hearing impairment PMID: 29325454
CIS interacted with phosphorylated EpoR at Y401, which was critical for the activation of STAT5 and ERK. PMID: 28038963
Studies indicate that suppressors of cytokine signaling (SOCS) proteins CIS, SOCS1, and SOCS3 can be considered the third immunocheckpoint molecules since they regulate cytokine signals that control the polarization of CD4(+) T cells and the maturation of CD8(+) T cells. PMID: 28188673
CISH rs414171 and rs6768300 variants might be associated with protection from pulmonary tuberculosis in Zahedan, Southeast Iran. PMID: 27266592
This study showed that there was significantly increased levels of CIS mRNA in elderly and Alzheimer's disease brains. PMID: 25286386
Data indicate that CIS protein stability is regulated through multiple mechanisms, including ubiquitination and interaction with Elongin B/C proteins, whereas CIS functional inhibition of PRLR signaling is dependent on the Elongin B/C interaction. PMID: 25448846
SOCS single nucleotide polymorphism is associated with breast cancer. PMID: 25104439
Study in Chinese Han population confirms that previously identified variants of CISH are associated with susceptibility to pulmonary tuberculosis. PMID: 25460819
CISH gene polymorphisms at -292 (rs414171) are associated with HBV clearance in HBeAg-positive CHB patients in the immune active phase, and AA is a favorable genotype for this effect. PMID: 24791876
Two SNPs (rs414171 and rs2239751) in the CISH gene were associated with persistent HBV infection in Han Chinese population PMID: 24964072
two genetic variants in CISH gene appear to increase susceptibility to TB in Chinese Han population PMID: 23949851
CISH promoter rs414171 and rs809451 polymorphisms may play a vital role in mediating individual susceptibility to tuberculosis PMID: 24632804
methylation of SOCS1, SOCS2, SOCS3 and CISH is infrequent in Ph-ve MPN. PMID: 24131863
data suggest FXR-mediated upregulation of cytokine inducible SH2-containing protein (CISH) may play an important role in the homeostasis of cytokine signal networks and be beneficial to control cytokine-associated inflammatory diseases PMID: 22817871
In GH transduction defect, abnormal GH signalling may be caused by over-expression of CIS, which may increase degradation of GHR PMID: 23426819
Suppressors of both cytokine signaling SOCS7 and cytokine-inducible SH2 protein (CIS) are expressed constitutively at higher levels in LNCaP-S17 (prostate cancer) cells than in LNCaP-C3 cells. PMID: 22213096
Study indicates the vital role of CISH single nucleotide polymorphism (SNP)-292A>T variant to hepatitis B virus infection in a Vietnamese population. PMID: 22033525
The authors conclude that Mycobacterium tuberculosis infection induces development of Tregs from CCR4(+) cells through a process that depends on PD-1and CISH. PMID: 21383382
Variants of CISH are associated with susceptibility to diseases caused by diverse infectious pathogens. PMID: 20484391
Low level of CISH expression in various tissues suggests that it is a novel prospective marker for diagnosis of colon cancer. PMID: 19807022
Up-regulation of cytokine inducible SH2-containing protein is associated with adult T-cell leukemia PMID: 14630083
CIS (cytokine-inducible SH2 protein)is binding with the conserved Y985 and Y1077 motifs in the cytosolic domain of the leptin receptor. PMID: 16684815
analysis of STAT5, CIS, and SOCS2 interactions with the growth hormone receptor PMID: 17666591
RACK1 has a role in protecting cancer cells from apoptosis by regulating the degradation of BimEL, which together with CIS could play an important role of drug resistance in chemotherapy PMID: 18420585
Elongin B/C recruitment regulates substrate binding by CIS PMID: 18508766
CISH expression in allergen-stimulated CD4(+) T cells from HE-allergic patients was significantly increased in both mRNA and protein levels compared with that from non-HE-allergic children. PMID: 18647318
CIS can serve as an SLE disease marker and may be involved in the pathogenesis of SLE, and that TNF-alpha may play an important role in the regulation of CIS PMID: 18820827
miR-98 and let-7 confer cholangiocyte expression of CIS in response to microbial challenge, a process that may be relevant to the regulation of TLR-mediated epithelial innate immune response. PMID: 19592657
These results suggest that RSV infection escapes the innate antiviral response by inducing SOCS1, SOCS3 or CIS expression in epithelial cells. PMID: 19595407

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Tissue Specificity

Expressed in various epithelial tissues. Abundantly expressed in liver and kidney, and to a lesser extent in lung. The tissue distribution of isoforms 1 and 1B is distinct.

Database Links

HGNC: 1984

OMIM: 602441

KEGG: hsa:1154

STRING: 9606.ENSP00000409346

UniGene: Hs.655334

Code	CSB-EP885721HU
Abbreviation	Recombinant Human CISH protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Cytokine-inducible SH2-containing protein (CISH)

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