Recombinant Human D-tyrosyl-tRNA (Tyr) deacylase 1 (DTD1)

Code CSB-YP819477HU
MSDS
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Source Yeast
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Code CSB-EP819477HU
MSDS
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Source E.coli
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Code CSB-EP819477HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819477HU
MSDS
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Source Baculovirus
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Code CSB-MP819477HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DTD1
Uniprot No.
Alternative Names
bA379J5.3; bA555E18.1; C20orf88; D tyrosyl tRNA deacylase 1 homolog; D-tyrosyl-tRNA(Tyr) deacylase 1; DNA unwinding element binding protein; DTD1; DTD1_HUMAN; DUE B; DUEB; HARS2; Histidyl tRNA synthetase 2; Histidyl-tRNA synthase-related; MGC119131; MGC41905; pqn 68
Species
Homo sapiens (Human)
Expression Region
1-209
Target Protein Sequence
MKAVVQRVTR ASVTVGGEQI SAIGRGICVL LGISLEDTQK ELEHMVRKIL NLRVFEDESG KHWSKSVMDK QYEILCVSQF TLQCVLKGNK PDFHLAMPTE QAEGFYNSFL EQLRKTYRPE LIKDGKFGAY MQVHIQNDGP VTIELESPAP GTATSDPKQL SKLEKQQQRK EKTRAKGPSE SSKERNTPRK EDRSASSGAE GDVSSEREP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Possible ATPase involved in DNA replication, may facilitate loading of CDC45 onto pre-replication complexes.; An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.
Gene References into Functions
  1. The state of DUE-B phosphorylation is maintained by the equilibrium between Cdc7-dependent phosphorylation and PP2A-dependent dephosphorylation. PMID: 25258324
  2. LDI-PCR revealed a fusion between DTD1 exon 4 and PDGFRB exon 12 in the cases with t(5;14)(q33;q32) and t(5;20)(q33;p11). PMID: 24772479
  3. DTD1 variants do not affect the abnormalities of the upper airways in aspirin-intolerant asthma patients PMID: 21479357
  4. The coordinated binding of DUE-B and Cdc45 to origins and the physical interactions of DUE-B, Cdc45, and TopBP1 suggest that complexes of these proteins are necessary for replication initiation. PMID: 20065034
  5. cloning and identification of novel cDNA which may be associated with FKBP25 PMID: 12392168
  6. DUE-B, a c-myc DNA-unwinding element-binding protein, plays an important role in replication in vivo. PMID: 15653697

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Subcellular Location
Nucleus. Cytoplasm. Note=Associated with chromatin at some replication origins containing functional DNA-unwinding elements (PubMed:20065034).
Protein Families
DTD family
Tissue Specificity
Expressed in many adult and fetal tissues. Highest levels in testis, ovary, spleen and in adult and fetal brain.
Database Links

HGNC: 16219

OMIM: 610996

KEGG: hsa:92675

STRING: 9606.ENSP00000366672

UniGene: Hs.659442

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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