Recombinant Human DNA-directed RNA polymerases I, II, and III subunit RPABC1 (POLR2E)

Code CSB-YP018332HU
MSDS
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Source Yeast
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Code CSB-EP018332HU
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Source E.coli
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Code CSB-EP018332HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018332HU
MSDS
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Source Baculovirus
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Code CSB-MP018332HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POLR2E
Uniprot No.
Alternative Names
and III subunit ABC1; and III subunit RPABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA directed RNA polymerase II 23 kDa polypeptide; DNA directed RNA polymerase II polypeptide E; DNA directed RNA polymerase II subunit E; DNA directed RNA polymerases I II and III subunit RPABC1; DNA-directed RNA polymerase II 23 kDa polypeptide; DNA-directed RNA polymerase II subunit E; DNA-directed RNA polymerases I; hRPB25; hsRPB5; II; Polr2e; Polymerase (RNA) II (DNA directed) polypeptide E (25kD); Polymerase (RNA) II (DNA directed) polypeptide E, 25kDa; RNA polymerases I; RNA polymerases I, II, and III subunit ABC1; RPAB1_HUMAN; RPABC1; RPB5; RPB5 homolog; XAP4
Species
Homo sapiens (Human)
Expression Region
1-210
Target Protein Sequence
MDDEEETYRL WKIRKTIMQL CHDRGYLVTQ DELDQTLEEF KAQSGDKPSE GRPRRTDLTV LVAHNDDPTD QMFVFFPEEP KVGIKTIKVY CQRMQEENIT RALIVVQQGM TPSAKQSLVD MAPKYILEQF LQQELLINIT EHELVPEHVV MTKEEVTELL ARYKLRENQL PRIQAGDPVA RYFGIKRGQV VKIIRPSETA GRYITYRLVQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process.
Gene References into Functions
  1. POLR2E rs3787016T allele was associated with an increased cancer risk in Chinese population PMID: 29724531
  2. POLR2E rs3787016 C/T and HULC rs7763881 were associated with a significantly decreased risk for ESCC 0.005). ANRIL rs2151280 T/C SNP was not associated with risk of ESCC. PMID: 25874495
  3. ELL has an early and essential role during rapid high-amplitude gene expression that is required for both Pol II pause site entry and release PMID: 22252557
  4. crystal of RPB5 diffracted to 2.8 A resolution and belonged to space group P4(3)2(1)2. PMID: 22102239
  5. RPB5-mediating protein is required for the proliferation of hepatocellular carcinoma cells. PMID: 21310960
  6. Results pinpoint critical residues for RNA polymerase II subunit 5 binding to RAP30 and/or to HBx, and identify these residues in both mammalian cells and in an in vitro binding assay. PMID: 16169872

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Subcellular Location
Nucleus.
Protein Families
Archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family
Database Links

HGNC: 9192

OMIM: 180664

KEGG: hsa:5434

STRING: 9606.ENSP00000215587

UniGene: Hs.24301

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