Recombinant Human DNA fragmentation factor subunit alpha(DFFA)

Code CSB-EP006737HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names DFFA
Uniprot No. O00273
Research Area Cell Biology
Alternative Names A330085O09Rik; Caspase activated deoxyribonuclease inhibitor short form; DFF 1; DFF 45; DFF alpha; DFF-45; DFF1; DFF35; DFF45; DFFA; Dffa DNA fragmentation factor, alpha subunit ; DFFA_HUMAN; DNA fragmentation factor 45 kDa subunit; DNA Fragmentation Factor Alpha Subunit; DNA fragmentation factor subunit alpha; DNA fragmentation factor, 45 kD, alpha subunit ; DNA fragmentation factor, 45kDa, alpha polypeptide (DFFA), transcript variant 1; DNA fragmentation factor, 45kDa, alpha polypeptide; DNA fragmentation factor, alpha subunit; DNAation factor 45 kDa subunit; H13; ICAD; ICAD L; ICAD S; Inhibitor of CAD; Inhibitor of Caspase Activated DNase; MGC143066; OTTHUMP00000001903 ; OTTHUMP00000001904 ; RP23 121D17.3
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-331aa
Target Protein Sequence MEVTGDAGVPESGEIRTLKPCLLRRNYSREQHGVAASCLEDLRSKACDILAIDKSLTPVTLVLAEDGTIVDDDDYFLCLPSNTKFVALASNEKWAYNNSDGGTAWISQESFDVDETDSGAGLKWKNVARQLKEDLSSIILLSEEDLQMLVDAPCSDLAQELRQSCATVQRLQHTLQQVLDQREEVRQSKQLLQLYLQALEKEGSLLSKQEESKAAFGEEVDAVDTGISRETSSDVALASHILTALREKQAPELSLSSQDLELVTKEDPKALAVALNWDIKKTETVQEACEWELALRLQQTQSLHSLRSISASKASPPGDLQNPKRARQDPT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 63.6kDa
Protein Length Full Length of BC007721
Tag Info N-terminal GST-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Inhibitor of the caspase-activated DNase (DFF40).
Gene References into Functions
  1. Glandular, menopause-independent DFF40, DFF45, and Bcl-2 overexpression may play an important role in the pathogenesis of endometrial polyps and benign endometrial hyperplasia PMID: 28914671
  2. evaluate the relative expression levels of miR-196a2 and three of its selected apoptosis-related targets; ANXA1, DFFA and PDCD4 in a sample of GI cancer patients PMID: 29091952
  3. Data show that the caspase-activated DNase (CAD) is activated when caspases cleave its endogenous inhibitor ICAD, resulting in the characteristic DNA laddering of apoptosis. PMID: 26106156
  4. Data suggest that DFF45 gene silencing, when applied in combination with doxorubicin, may offer a therapeutic strategy for the treatment of breast cancer. PMID: 24277473
  5. ICAD deficiency was associated with severe genomic instability. PMID: 23451280
  6. mRNA splicing is actively driven toward the pro-apoptotic isoforms of Bim, Bcl-x, and ICAD in Pnn-depleted MCF-7 cells. PMID: 22454513
  7. The DFF45 level in human endometrium corresponds to the respective phase of the menstrual cycle and decreases significantly after menopause. PMID: 22378161
  8. study reveals a previously unrecognized function of miR-145 in DFF45 processing, which may underlie crucial aspects of cancer biology PMID: 20687965
  9. The heterodimer, DFF40-DFF45, is localized to the chromatin fraction under apoptotic as well as non-apoptotic conditions. PMID: 19882353
  10. DFF45 at chromosome 1 reveal rare allelic variants in neuroblastoma tumors PMID: 11870543
  11. NMR solution structure of the C-terminal domain of DFF45, which is essential for its chaperone-like activity PMID: 12144788
  12. Hypoxia-induced cleavage of caspase-3 and DFF45/ICAD in human failed cardiomyocytes. PMID: 12181128
  13. subunit structures and stoichiometries in human cells before and after induction of apoptosis PMID: 12748178
  14. Hepatitis C virus core protein increases a steady-state level of ICAD protein, possibly through enhancing its promoter activity PMID: 14675622
  15. apoptotic DNA fragmentation factor is required for the maintenance of genetic stability and may play a role in tumor suppression PMID: 16432220
  16. plays an important and P53-independent role in maintaining chromosome stability and suppressing tumor development PMID: 16619042
  17. demonstrate the cellular mechanisms of neuronal cell degeneration induced via c-Jun-N-terminal kinases and caspase-dependent signaling PMID: 17645689
  18. C-terminal region of each subunit, DFF40 (RLKRK) and DFF45 (KRAR), is essential for nuclear accumulation of the DFF complex. PMID: 17938174
  19. Interestingly, nuclear DNA fragmentation occurred and consistently DNA fragmentation factor (DFF45)/Inhibitor of caspase-activated DNase (ICAD) was cleaved inside the cell as well as in vitro, suggesting a role of caspase-2 in nuclear DNA fragmentation. PMID: 17945178
  20. The highest level of DFF45 endometrial expression was found during the early secretory cycle phase, and significantly lower DFF45 expression was found in the endometrium during the mid-secretory as compared to the early secretory cycle phase. PMID: 18292826
  21. identified a TATA-less region upstream of the transcription start site as a basal promoter of the ICAD gene. An E-Box motif, which binds transcription factors of the basic helix-loop-helix/leucine zipper family, is responsible for transcriptional activity PMID: 18500556
  22. MPP(+) did not change the total levels of c-Jun but enhanced phosphorylation of c-Jun at Ser73 and cleavage of DNA fragmentation factor 45, which were diminished by selegiline. PMID: 18805449
  23. A decreased level of DFF45 observed in ovarian endometriosis may be a part of an apoptosis-resistant mechanism enhancing the disease progression. PMID: 19535198

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Subcellular Location Cytoplasm
Database Links

HGNC: 2772

OMIM: 601882

KEGG: hsa:1676

STRING: 9606.ENSP00000366237

UniGene: Hs.484782

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