DNA fragmentation factor subunit alpha is a protein in humans that is encoded by DFFA gene. Inhibitor of the caspase-activated DNase (DFF40).
The following DFFA reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
DFFA Antibodies for Homo sapiens (Human)
|Code||Product Name||Species Reactivity||Application|
|CSB-PA006737DSR2HU||Human||ELISA, WB, IHC, IP|
|CSB-PA000044||Human||WB, IHC, ELISA|
|CSB-PA002996||Human,Mouse,Rat||WB, IHC, IF, ELISA|
DFFA Proteins for Homo sapiens (Human)
In Vivo Biotinylation in E.coli
DFFA Proteins for Mus musculus (Mouse)
In Vivo Biotinylation in E.coli
DNA fragmentation factor subunit alpha (DFFA) is one of the two subunits of the heterodimeric protein DNA Fragmentation Factor (DFF), a major endonuclease responsible for chromosomal DNA cleavage during apoptosis . It is also called DNA fragmentation factor 45 kDa subunit (DFF45) because its molecular weight is 45 kDa. DFF45 is indispensable for the expression and folding of DFF40, thus acting as a molecular chaperone . In non-apoptotic cells, DFF45 exists as a heterodimeric complex with DFF40. Upon activation of apoptosis, DFF45 is cleaved by caspase 3 and then dissociates from DFF40, another subunit of the DFF . Removal of DFF45 releases and activates DFF40 . In other words, DFF45 suppresses the activity of DFF40, a caspase-activated DNase, or nuclease (CAD or CPAN) . So DFF45 is named the inhibitor of the caspase-activated DNase (ICAD), too. Active DFF40 is capable of cleaving chromosomal DNA into oligonucleosomal size fragments . The biochemical hallmark of apoptosis is the cleavage of chromatin into nucleosomal fragments . Studies showed that caspase 7 and granzyme B can also process DFF45 during apoptosis . DFF35 (or ICAD-S), a short form of DFF45, lacks C-terminus and consists of amino acids 1-65 of DFF45, and is produced by alternative splicing and is expressed in numerous different cells . DFF35/ICAD-S also inhibits DFF40/CAD activity but lacks the chaperon function . Decreased DFF45 expression has been observed in renal, colorectal, and esophageal cancers. It has been postulated that the downregulation of DFF45 may play a role in tumor escape from apoptosis and may promote tumor progression and metastasis .
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 Thomas D, Du C, et al. DFF45/ICAD can be directly process by granzyme B during the induction of apoptosis [J]. Immunity 2000; 12:621-2.
 M Enari, H Sakahira, et al. A Caspase-Activated DNase That Degrades DNA During Apoptosis, and Its Inhibitor ICAD [J]. Nature, 1998, 391 (6662), 43-50.
 Sabol, S.L., et al. Inhibition of apoptosis-associated DNA fragmentation activity in nonapoptotic cells: the role of DNA fragmentation factor-45(DFF45/ICAD) [J]. Biochem. Biophys. Res. Commun. 1998, 253: 151-58.
 Kawane, K., H. Fukuyama, et al. Structure and promoter analysis of murine CAD and ICAD genes [J]. Cell Death Differ. 1999, 6: 745-52.
 Gu, J., R.P. Dong, et al. Functional interaction of DFF35 and DFF45 with caspase-activated DNA fragmentation nuclease DFF40 [J]. J. Biol. Chem. 1999, 274: 20759-0762.
 Sakahira, H., M. Enari, et al. Functional differences of two forms of the inhibitor of caspase-activated DNase, ICAD-L and ICAD-S [J]. J. Biol. Chem. 1999, 274: 15740-5744.
 Rajandram R, Razack AH, et al. Decreased Expression of Inhibitor of Caspase-Activated DNase (ICAD) in Renal Cell Carcinoma - Tissue Microarray of Human Samples [J]. J Kidney Cancer VHL. 2016; 3(1):1-11.
 Konishi S, Ishiguro H, Shibata Y, et al. Decreased expression of DFF45/ICAD is correlated with a poor prognosis in patients with esophageal carcinoma. Cancer. 2002;95(12):2473-478.
 Errami Y, Brim H, Oumouna-Benachour K, et al. ICAD deficiency in human colon cancer and predisposition to colon tumorigenesis: linkage to apoptosis resistance and genomic instability. PLoS One. 2013;8(2):e57871.