Recombinant Human DNA polymerase subunit gamma-2, mitochondrial (POLG2)

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Code CSB-EP883399HU
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP883399HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) POLG2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP883399HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) POLG2.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
POLG2
Uniprot No.
Research Area
Transcription
Alternative Names
DNA directed DNA polymerase gamma 2; DNA polymerase gamma accessory 55 kDa subunit; DNA polymerase subunit gamma 2; mitochondrial; DNA polymerase subunit gamma-2; DPOG2_HUMAN; HP55; mitochondrial; Mitochondrial DNA polymerase accessory subunit; Mitochondrial DNA polymerase subunit gamma 2; Mitochondrial polymerase gamma 2; MtPolB; OTTMUSP00000003758; p55; PEOA4; POLB; PolG beta; PolG-beta; POLG2; POLGB; Polymerase (DNA directed); gamma 2; accessory subunit; Polymerase; gamma 2; RP23-247J12.3
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-485aa
Target Protein Sequence
MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALLEICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLPGDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQIGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQDEEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYLYDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPGYLETMQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
70.9kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Mitochondrial polymerase processivity subunit. It regulates the polymerase and exonuclease activities promoting processive DNA synthesis. Binds to ss-DNA.
Gene References into Functions
  1. This is the first report of a patient with a homozygous mutation in POLG2 and with a clinical presentation of severe hepatic failure and mitochondrial depletion. PMID: 27592148
  2. Results show that polymorphisms at POLG2 and POLRMT increased risk of oral cancer and leukoplakia, respectively, probably modulating synthesis and activity of the enzymes. PMID: 26403317
  3. Mitochondrial DNA (mtDNA) content plays an important role in energy production and sustaining normal physiological function. PMID: 24524965
  4. The authors describe the molecular characterization of a potential dominant POLG2 mutation (p.R369G) in a patient with autosomal dominant progressive external ophthalmoplegia and multiple mtDNA deletions. PMID: 22155748
  5. Data show that rs17650301 in POLG2 is a good candidate marker for UBC invasiveness in Japanese males. PMID: 21734712
  6. The biochemical analysis helps explain the pathogenesis of POLG2 mutations in mitochondrial disease. PMID: 21555342
  7. human pol gammaB exhibits a catalytic subunit- and substrate DNA-dependent dimerization. PMID: 19858216
  8. PolB gamma can bind double-stranded DNA and may play a role in DNA replication or repair PMID: 12379656
  9. Study represents the first structure-function analysis of the thumb subdomain in pol gamma and examines the consequences of mitochondrial disease mutations in this region. PMID: 19478085
  10. Both increased and decreased expression of POLGbeta altered nucleoid structure and precipitated a marked decrease in 7S DNA molecules, which form short displacement-loops on mitochondrial DNA. PMID: 19625489

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Involvement in disease
Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 4 (PEOA4)
Subcellular Location
Mitochondrion.
Database Links

HGNC: 9180

OMIM: 604983

KEGG: hsa:11232

STRING: 9606.ENSP00000442563

UniGene: Hs.437009

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