Recombinant Human DNA polymerase subunit gamma-2, mitochondrial (POLG2), partial

Code CSB-YP883399HU
MSDS
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Source Yeast
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Code CSB-EP883399HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP883399HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP883399HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
POLG2
Uniprot No.
Alternative Names
DNA directed DNA polymerase gamma 2; DNA polymerase gamma accessory 55 kDa subunit; DNA polymerase subunit gamma 2; mitochondrial; DNA polymerase subunit gamma-2; DPOG2_HUMAN; HP55; mitochondrial; Mitochondrial DNA polymerase accessory subunit; Mitochondrial DNA polymerase subunit gamma 2; Mitochondrial polymerase gamma 2; MtPolB; OTTMUSP00000003758; p55; PEOA4; POLB; PolG beta; PolG-beta; POLG2; POLGB; Polymerase (DNA directed); gamma 2; accessory subunit; Polymerase; gamma 2; RP23-247J12.3
Species
Homo sapiens (Human)
Expression Region
-
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Mitochondrial polymerase processivity subunit. It regulates the polymerase and exonuclease activities promoting processive DNA synthesis. Binds to ss-DNA.
Gene References into Functions
  1. This is the first report of a patient with a homozygous mutation in POLG2 and with a clinical presentation of severe hepatic failure and mitochondrial depletion. PMID: 27592148
  2. Results show that polymorphisms at POLG2 and POLRMT increased risk of oral cancer and leukoplakia, respectively, probably modulating synthesis and activity of the enzymes. PMID: 26403317
  3. Mitochondrial DNA (mtDNA) content plays an important role in energy production and sustaining normal physiological function. PMID: 24524965
  4. The authors describe the molecular characterization of a potential dominant POLG2 mutation (p.R369G) in a patient with autosomal dominant progressive external ophthalmoplegia and multiple mtDNA deletions. PMID: 22155748
  5. Data show that rs17650301 in POLG2 is a good candidate marker for UBC invasiveness in Japanese males. PMID: 21734712
  6. The biochemical analysis helps explain the pathogenesis of POLG2 mutations in mitochondrial disease. PMID: 21555342
  7. human pol gammaB exhibits a catalytic subunit- and substrate DNA-dependent dimerization. PMID: 19858216
  8. PolB gamma can bind double-stranded DNA and may play a role in DNA replication or repair PMID: 12379656
  9. Study represents the first structure-function analysis of the thumb subdomain in pol gamma and examines the consequences of mitochondrial disease mutations in this region. PMID: 19478085
  10. Both increased and decreased expression of POLGbeta altered nucleoid structure and precipitated a marked decrease in 7S DNA molecules, which form short displacement-loops on mitochondrial DNA. PMID: 19625489

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Involvement in disease
Progressive external ophthalmoplegia with mitochondrial DNA deletions, autosomal dominant, 4 (PEOA4)
Subcellular Location
Mitochondrion.
Database Links

HGNC: 9180

OMIM: 604983

KEGG: hsa:11232

STRING: 9606.ENSP00000442563

UniGene: Hs.437009

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