Recombinant Human DNA repair protein RAD50 (RAD50), partial

1. Expression levels of MRN complex proteins(MRE11/RAD50/NBS1) significantly predict disease-free survival in rectal cancer patients, including those treated with neoadjuvant radiotherapy, and may have value in the management of these patients. PMID: 30176843
2. These data suggest that RAD50 foci formation in CTCs and CAMLs may be used to track cells subjected to radiation occurring at primary tumors, and following PD-L1 expression in circulating cells may be used as a surrogate for tracking adaptive changes in immunotherapeutic targets. PMID: 28679765
3. These evidences suggest that NBS1 is regulated by two kind of mechanisms: complex formation dependent on ATM, and protein degradation mediated by an unknown MG132-resistant pathway. PMID: 28369484
4. Five out of twelve patients with defects in either of MSH2, RAD50 and NBN genes suffered from rare life-threatening AE, more frequently than in control group (p = 0.0005). When all detected variants were taken into account, the majority of patients (8 out of 15) suffered from life-threatening toxicity during chemotherapy. PMID: 28376765
5. Low RAD50 expression is associated with low-grade epithelial ovarian cancer. PMID: 28073364
6. although recruitment of the MRE11-RAD50-NBS1 (MRN) DSB-sensing complex to viral genomes and activation of the ATM kinase can promote KSHV replication, proteins involved in nonhomologous end joining (NHEJ) repair restrict amplification of viral DNA. PMID: 28855246
7. Mre11-Rad50-Nbs1 complex initiates DNA double strand break repair. PMID: 28867292
8. symmetrical engagement of the Rad50 catalytic head domains with ATP bound at both sites is important for MRN functions in eukaryotic cells. PMID: 28369545
9. The results illuminate the important role of Nbs1 and CtIP in determining the substrates and consequences of human Mre11/Rad50 nuclease activities on protein-DNA lesions. PMID: 27814491
10. identification of a novel association for longevity in the RAD50/IL13 region on chromosome 5q31.1; the lead SNP rs2706372 is located in the intronic region of the RAD50 gene and is in strong linkage disequilibrium with other associated SNPs close to IL13 and IL5 PMID: 27004735
11. the structure of the human Rad50 hook and coiled-coil domains, was determined. PMID: 28134932
12. although Mre11 is required for efficient HR-dependent repair of ionizing-radiation-induced DSBs, Mre11 is largely dispensable for DSB resection in both chicken DT40 and human TK6 B cell lines. PMID: 27311583
13. The high expression of MRE11-RAD50-NBS1 complex constituents could be a predictor for poor prognosis and chemoresistance in gastric cancer PMID: 27798884
14. Germline mutation in RAD50 gene is associated with familial breast cancer. PMID: 26824983
15. The MRN complex is essential to restrain MYCN-induced replication stress during neural cell proliferation. PMID: 26068589
16. The C allele of rs6871536 was positively associated with atopic asthma in a Chinese population. PMID: 26493291
17. study identified the copy number deletion of RAD50 as a candidate marker for survival PMID: 27016230
18. Lack of association between RAD50-IL13 polymorphisms and pediatric asthma susceptibility in Northeastern Han Chinese PMID: 26365633
19. we present an in-depth genomic and functional genetic analysis identifying RAD50 hypomorphism as a contributing factor to a curative response to systemic combination therapy in a patient with recurrent, metastatic small-cell cancer. PMID: 24934408
20. a significant increase in DKC1, RAD50, MRE11 and RPA1 expression in MM cases with high bone marrow infiltration (pPMID: 26366868
21. RAD50 is required for ataxia-telangiectasia and Rad3-related (ATR) activation in mammalian cells in response to DNA replication stress. It is in turn phosphorylated at a specific site (S635) by ATR, which is required for ATR signaling through Chk1. PMID: 24694934
22. These results suggest that MRE11 is important in maintaining the structural arrangement of RAD50 in the protein complex and perhaps has a role in reinforcing proper alignment of the coiled coils in the ATP-bound state. PMID: 25828805
23. Furthermore, they collectively help to explain how MRN regulates DNA repair pathway choice. [review] PMID: 25576492
24. The results did not confirm the previously reported association between the RAD50 SNP Rs2237060 and the development of either common variable immunodeficiency or selective IgA deficiency. PMID: 26310047
25. ). We find that RAD50 and INPP4B expression levels have a synergistic influence on breast cancer survival, possibly through their effects on treatment response. PMID: 25528023
26. SNP (rs17166050) in RAD50 gene is associated with the susceptibility to acute lymphoid leukemia in children PMID: 26014697
27. This work demonstrates that the Mre11-Rad50-Nbs1 DNA repair complex positively regulates AAV replication and plays a role in the integration of adeno-associated airus in the presence of herpes simplex virus 1. PMID: 25903339
28. ATP switches the Mre11-Rad50-Nbs1 repair factor between signaling and processing of DNA ends. (Review) PMID: 25213441
29. Results suggest that BRCA1 participates in ALT through its interactions with RAD50 and BLM. PMID: 25084169
30. These data establish that MRE11A, RAD50, and NBN are intermediate-risk breast cancer susceptibility genes. PMID: 24894818
31. These results articulate a model of inhibition of adeno-associated virus gene expression in which physical interaction of viral DNA with Mre11/Rad50/Nbs1 complex is more important than enzymatic activity. PMID: 25320294
32. RAD50 abrogation impairs DNA damage response and sensitizes breast cancer cells to cisplatin-combined therapies. PMID: 24642965
33. this study reports a direct cytosolic interaction between the DNA-damage sensor Rad50 and the innate immune system adaptor CARD9. PMID: 24777530
34. Common genetic variations in the MRE11 and RAD50 genes do not contribute to an increased risk of laryngeal cancer. PMID: 24079363
35. The formerly reported sequence variants in the RAD50 and MRE11 gene may not constitute a risk factor of childhood ALL in Polish population. PMID: 24093751
36. Rad17 is phosphorylated by ATM at Thr622 resulting in a direct interaction of Rad17 with NBS1, facilitating recruitment of MRE11, RAD50 and ATM to the DNA double-strand breaks. PMID: 24534091
37. Immunohistochemistry was performed for MRE11, RAD50 and NIBRIN PMID: 23954013
38. The RAD50 gene was screened for mutations in well-characterized, Finnish, high-risk hereditary breast and/or ovarian cancer individuals. PMID: 21356067
39. The Mre11-Rad50-Nbs1 (MRN) complex further stimulates resection in the presence of Ku and DNA-PKcs by recruiting Exo1 and enhancing DNA-PKcs autophosphorylation, and it also inhibits DNA ligase IV/XRCC4-mediated end rejoining. PMID: 24220101
40. The association of asthma with variation near RAD50/IL13, first discovered in individuals of European ancestry, replicates in individuals of African ancestry. PMID: 23666277
41. Ataxia telangiectasia-mutated (ATM) kinase activity is regulated by ATP-driven conformational changes in the Mre11/Rad50/Nbs1 (MRN) complex. PMID: 23525106
42. Rad50 zinc hook is important for the Mre11 complex to bind chromosomal DNA double-stranded breaks and initiate various DNA damage responses PMID: 22833675
43. Adenovirus 5 regulates sumoylation of Mre11-Rad50-Nbs1 components through a paralog-specific mechanism. PMID: 22740413
44. The staining features of Rad50, a component of an essential DNA double-strand break repair complex, are clearly increased in melanoma cells with regards to both staining intensity and the number of positive melanoma cells PMID: 22725639
45. germline variants of RAD50 and NBS1 in non-BRCA1/2 high-risk families were not correlated with a breast cancer susceptibility in a cohort of Chinese women. PMID: 21811815
46. Carriers of the RAD50 haplotype may be susceptible to the DNA-damaging effects of radiation therapy associated with radiation-induced breast cancer. PMID: 21898661
47. Human Ku70/80 protein blocks exonuclease 1-mediated DNA resection in the presence of human Mre11 or Mre11/Rad50 protein complex. PMID: 22179609
48. RAD50, DNA-PKcs kinase activity, and transcription context are each important to limit incorrect end use during EJ repair of multiple DSBs, but each has distinct roles during repair events requiring end processing PMID: 22027841
49. Studies indicate that RAD50 and PTPRE of crude associations with asthma at a Bonferroni-corrected level of significance, while IL4R, CCL5 and TBXA2R of nominal significance. PMID: 21734400
50. phosphorylation of Rad50 plays a key regulatory role as an adaptor for specific ATM-dependent downstream signaling through SMC1 for DNA repair and cell cycle checkpoint control in the maintenance of genome integrity. PMID: 21757780

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HGNC: 9816

OMIM: 604040

KEGG: hsa:10111

STRING: 9606.ENSP00000265335

UniGene: Hs.633509