Recombinant Human DNA repair protein RAD51 homolog 4 (RAD51D)

Code CSB-YP019268HU
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Source Yeast
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Code CSB-EP019268HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019268HU
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Source Baculovirus
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Code CSB-MP019268HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RAD51D
Uniprot No.
Alternative Names
BROVCA4; DNA repair protein RAD51 homolog 4; HsTRAD; OTTHUMP00000163851; OTTHUMP00000163852; OTTHUMP00000163853; R51H3; RA51D_HUMAN; RAD51 homolog D (S. cerevisiae); RAD51 homolog D; RAD51 like 3 (S. cerevisiae); RAD51 paralog D; RAD51, S. cerevisiae, homolog of, D; RAD51-like protein 3; Rad51l3; Recombination repair protein; S. cerevisiae RAD51-like 3; TRAD
Species
Homo sapiens (Human)
Expression Region
1-328
Target Protein Sequence
MGVLRVGLCP GLTEEMIQLL RSHRIKTVVD LVSADLEEVA QKCGLSYKAL VALRRVLLAQ FSAFPVNGAD LYEELKTSTA ILSTGIGSLD KLLDAGLYTG EVTEIVGGPG SGKTQVCLCM AANVAHGLQQ NVLYVDSNGG LTASRLLQLL QAKTQDEEEQ AEALRRIQVV HAFDIFQMLD VLQELRGTVA QQVTGSSGTV KVVVVDSVTA VVSPLLGGQQ REGLALMMQL ARELKTLARD LGMAVVVTNH ITRDRDSGRL KPALGRSWSF VPSTRILLDT IEGAGASGGR RMACLAKSSR QPTGFQEMVD IGTWGTSEQS ATLQGDQT
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. Bind to single-stranded DNA (ssDNA) and has DNA-dependent ATPase activity. Part of the Rad21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Involved in telomere maintenance. The BCDX2 subcomplex XRCC2:RAD51D can stimulate Holliday junction resolution by BLM.
Gene References into Functions
  1. Contribution of RAD51D germline mutations in breast and ovarian cancer in Greece. PMID: 30111881
  2. Results highlight the importance of a functional RAD51D-XRCC2 interaction to promote HR and prevent the development of HGSC. PMID: 28646019
  3. variants in RAD51D were associated with moderate risks of breast cancer. PMID: 28418444
  4. of Rad51d mediated by E3 Ligase Rnf138 has a role in the homologous recombination repair pathway PMID: 27195665
  5. endogenous regulation of RAD51D by miR-103/107 was observed in several tumor subtypes; both miR-103 and miR-107 directly target and regulate RAD51 and RAD51D PMID: 24088786
  6. We aimed to determine the prevalence of germline RAD51D mutations in Spanish breast and ovarian cancer families negative for BRCA1/BRCA2 PMID: 24130102
  7. Our data provide additional evidence that RAD51D mutations are enriched among ovarian cancer patients, but are extremely rare among familial breast cancer patients. PMID: 23372765
  8. loss-of-function mutations in RAD51D predispose to ovarian carcinoma but not to breast carcinoma. PMID: 22986143
  9. RAD51D is primarily a moderate penetrance susceptibility gene for ovarian cancer, with clinical significance for the carriers. PMID: 22652533
  10. The RAD51D should be included in genetic screening of ovarian cancer families that do not have BRCA1/BRCA2 mutations. PMID: 22415235
  11. These data indicate that RAD51D mutation testing may have clinical utility in individuals with ovarian cancer and their families. PMID: 21822267
  12. The N-terminal domain of Rad51D is required for the ssDNA-specific binding function of human Rad51D. PMID: 21111057
  13. Polymorphisms in RAD51D gene is associated with breast cancer. PMID: 20054644
  14. Homologous pairing and ring and filament structure formation activities of the human Xrcc2*Rad51D complex PMID: 11834724
  15. RAD51L3 cooperates with Bloom Syndrome Protein during the late stages of homologous recombination processes that serve to restore productive DNA replication at sites of damaged or stalled replication forks PMID: 12975363
  16. Telomere maintenance requires RAD51D. PMID: 15109494
  17. E233G single nucleotide polymorphism is a low-penetrance susceptibility gene in the specific subgroup of high-risk familial breast cancer cases that are not related to BRCA1/2. PMID: 15170666
  18. Interactions between RAD51D and its XRCC2 and RAD51C partners require a functional RAD51D Walker B ATPase motif, but not motif A. PMID: 16717288
  19. The RAD51D E233G variant is not associated with breast cancer. PMID: 18058226

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Involvement in disease
Breast-ovarian cancer, familial, 4 (BROVCA4)
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Chromosome, telomere.
Protein Families
RecA family, RAD51 subfamily
Tissue Specificity
Expressed in colon, prostate, spleen, testis, ovary, thymus and small intestine. Weakly expressed in leukocytes.
Database Links

HGNC: 9823

OMIM: 602954

KEGG: hsa:5892

STRING: 9606.ENSP00000466834

UniGene: Hs.631757

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