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Recombinant Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial (DUT)

Recombinant Human Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial (DUT)

Code	CSB-YP843660HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP843660HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP843660HU-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP843660HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP843660HU
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

DUT

Uniprot No.

P33316

Alternative Names

Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTPase; EC 3.6.1.23; dUTP pyrophosphatase; DUT

Species

Homo sapiens (Human)

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Gene References into Functions

A single homozygous missense mutation (chr15.hg19:g.48,626,619A>G) located in the dUTPase (DUT) gene was associated with a syndrome of bone marrow failure and diabetes. PMID: 28073829
Comparison of the cellular distribution of wild-type dUTPase with those of hyperphosphorylation- and hypophosphorylation-mimicking mutants suggests that phosphorylation at Ser11 leads to the exclusion of dUTPase from the nucleus. PMID: 24311590
EBV-encoded dUTPase may act as an intercellular signaling molecule capable of modulating the cellular microenvironment PMID: 23894549
Data sugget that inhibition of dUTPase is a mechanism-based therapeutic approach to significantly enhance the efficacy of thymidylate synthase (TS)-targeted chemotherapeutic agents. PMID: 22172489
Repression of dUTPase induced specific expression level increments for thymidylate kinase and thymidine kinase, and also an increased sensitization to 5-fluoro-2'-deoxyuridine and 5-fluoro-uracil. PMID: 21780905
High dUTP pyrophosphatase is associated with recurrence in colorectal cancer patients undergoing 5-fluorouracil adjuvant chemotherapy PMID: 20560137
nuclear dUTPase may be a good biomarker for predicting prognosis in HCC patients after surgical resection PMID: 19968781
significant differences between the human and Plasmodium dUTPases PMID: 19879316
Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil. PMID: 12096336
Nuclear dUTPase may contain a complex nuclear localization signal that is located throughout the entire protein. PMID: 12799180
dUTPase staining provides a useful measure of cell proliferation distinct from that offered by Ki-67 labeling. It proved particularly useful for the evaluation of diffuse astrocytomas. PMID: 16325515
kinetic model of the human dUTPase catalytic cycle PMID: 17848562
determined the crystal structure of the enzyme:alpha,beta-imino-dUTP PMID: 17880943
Establishment of a direct role for both mutant and wild-type forms of p53 in modulating dUTPase promoter activity. PMID: 19015155
hinge proline destabilize human and Escherichia coli dUTPases without preventing trimeric organization PMID: 19302784

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Subcellular Location

[Isoform 2]: Nucleus.; [Isoform 3]: Mitochondrion.

Protein Families

DUTPase family

Tissue Specificity

Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein leve

Database Links

HGNC: 3078

OMIM: 601266

KEGG: hsa:1854

STRING: 9606.ENSP00000370376

UniGene: Hs.527980

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