DUT Antibody

Datasheet

Code	CSB-PA926314
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using CSB-PA926314(DUT Antibody) at dilution 1/30, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 10%SDS-PAGE, Lysate: 40 μg, Lane: 293T cells, Primary antibody: CSB-PA926314(DUT Antibody) at dilution 1/500, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

P33316

Target Names

DUT

Alternative Names

Deoxyuridine 5' triphosphate nucleotidohydrolase; nuclear isoform antibody; Deoxyuridine 5'-triphosphate nucleotidohydrolase; mitochondrial antibody; Deoxyuridine triphosphatase antibody; dut antibody; DUT_HUMAN antibody; dUTP nucleotidohydrolase antibody; dUTP pyrophosphatase antibody; dUTPase antibody; FLJ20622 antibody

Raised in

Rabbit

Species Reactivity

Human,Rat

Immunogen

Synthetic peptide of Human DUT

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:1000-1:2000
WB	1:200-1:1000
IHC	1:10-1:50

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

Gene References into Functions

A single homozygous missense mutation (chr15.hg19:g.48,626,619A>G) located in the dUTPase (DUT) gene was associated with a syndrome of bone marrow failure and diabetes. PMID: 28073829
Comparison of the cellular distribution of wild-type dUTPase with those of hyperphosphorylation- and hypophosphorylation-mimicking mutants suggests that phosphorylation at Ser11 leads to the exclusion of dUTPase from the nucleus. PMID: 24311590
EBV-encoded dUTPase may act as an intercellular signaling molecule capable of modulating the cellular microenvironment PMID: 23894549
Data sugget that inhibition of dUTPase is a mechanism-based therapeutic approach to significantly enhance the efficacy of thymidylate synthase (TS)-targeted chemotherapeutic agents. PMID: 22172489
Repression of dUTPase induced specific expression level increments for thymidylate kinase and thymidine kinase, and also an increased sensitization to 5-fluoro-2'-deoxyuridine and 5-fluoro-uracil. PMID: 21780905
High dUTP pyrophosphatase is associated with recurrence in colorectal cancer patients undergoing 5-fluorouracil adjuvant chemotherapy PMID: 20560137
nuclear dUTPase may be a good biomarker for predicting prognosis in HCC patients after surgical resection PMID: 19968781
significant differences between the human and Plasmodium dUTPases PMID: 19879316
Novel gain of function activity of p53 mutants: activation of the dUTPase gene expression leading to resistance to 5-fluorouracil. PMID: 12096336
Nuclear dUTPase may contain a complex nuclear localization signal that is located throughout the entire protein. PMID: 12799180
dUTPase staining provides a useful measure of cell proliferation distinct from that offered by Ki-67 labeling. It proved particularly useful for the evaluation of diffuse astrocytomas. PMID: 16325515
kinetic model of the human dUTPase catalytic cycle PMID: 17848562
determined the crystal structure of the enzyme:alpha,beta-imino-dUTP PMID: 17880943
Establishment of a direct role for both mutant and wild-type forms of p53 in modulating dUTPase promoter activity. PMID: 19015155
hinge proline destabilize human and Escherichia coli dUTPases without preventing trimeric organization PMID: 19302784

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Subcellular Location

[Isoform 2]: Nucleus.; [Isoform 3]: Mitochondrion.

Protein Families

DUTPase family

Tissue Specificity

Found in a variety of tissues. Isoform 3 expression is constitutive, while isoform 2 expression correlates with the onset of DNA replication (at protein level). Isoform 2 degradation coincides with the cessation of nuclear DNA replication (at protein leve

Database Links

HGNC: 3078

OMIM: 601266

KEGG: hsa:1854

STRING: 9606.ENSP00000370376

UniGene: Hs.527980

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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