Recombinant Human Diacylglycerol kinase kappa (DGKK), partial

Code CSB-YP702513HU
MSDS
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Source Yeast
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Code CSB-EP702513HU
MSDS
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Source E.coli
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Code CSB-EP702513HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP702513HU
MSDS
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Source Baculovirus
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Code CSB-MP702513HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DGKK
Uniprot No.
Alternative Names
DGKKDiacylglycerol kinase kappa; DAG kinase kappa; EC 2.7.1.107; 142 kDa diacylglycerol kinase; Diglyceride kinase kappa; DGK-kappa
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Diacylglycerol kinase that converts diacylglycerol/DAG into phosphatidic acid/phosphatidate/PA and regulates the respective levels of these two bioactive lipids. Thereby, acts as a central switch between the signaling pathways activated by these second messengers with different cellular targets and opposite effects in numerous biological processes (Probable).
Gene References into Functions
  1. FMRP is mostly associated diacylglycerol kinase kappa (Dgkkappa), a master regulator that controls the switch between diacylglycerol and phosphatidic acid signaling pathways. PMID: 27233938
  2. Dgkk appears to be a marker or mediator of squamous cell differentiation in development of mouse external genitalia. No association exists between Dgkk expression and formation of preputial cleft in the genital tubercle of diethylsilbestrol treated mice. PMID: 26162297
  3. Studied the relationship between DGKK single nucleotide polymorphisms (SNPs) and hypospadias risk in the Han Chinese population. PMID: 25327554
  4. Common variants in DGKK are strongly associated with risk of hypospadias PMID: 21113153
  5. DGK kappa is a novel human type II diacylglycerol kinase PMID: 16210324

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Subcellular Location
Cell membrane; Peripheral membrane protein.
Protein Families
Eukaryotic diacylglycerol kinase family
Tissue Specificity
Expressed in testis, and to a lesser extent in placenta.
Database Links

HGNC: 32395

OMIM: 300837

KEGG: hsa:139189

UniGene: Hs.38132

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