Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

DPYSL3

Uniprot No.

Q14195

Research Area

Neuroscience

Alternative Names

Collapsin response mediator protein 4; Collapsin response mediator protein 4 long; CRMP 4; CRMP-4; CRMP4; Dihydropyrimidinase like 3; Dihydropyrimidinase related protein 3; Dihydropyrimidinase-related protein 3; DPYL3_HUMAN; DPYSL3; DRP 3; DRP-3; DRP3; LCRMP; TUC4; ULIP 1; ULIP; ULIP-1; ULIP1; UNc 33 like phosphoprotein 1; Unc 33 like phosphoprotein; Unc-33-like phosphoprotein 1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-570aa

Target Protein Sequence

MSYQGKKNIPRITSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVIPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLIKDKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQTRMLEMGITGPEGHVLSRPEELEAEAVFRAITIASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRISVGSDSDLVIWDPDAVKIVSAKNHQSAAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSARGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

66.0 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 1-570 constitute the expression domain of recombinant Human DPYSL3. The theoretical molecular weight of the DPYSL3 protein is 66.0 kDa. This DPYSL3 recombinant protein is manufactured in e.coli. Fusion of the N-terminal 6xHis tag into the DPYSL3 encoding gene fragment was conducted, allowing for easier detection and purification of the DPYSL3 protein in subsequent stages.

Human dihydropyrimidinase-related protein 3 (DPYSL3) is a cytoplasmic protein involved in neurite outgrowth and axon guidance during neuronal development. DPYSL3 functions as a collapsin response mediator, influencing cytoskeletal dynamics and neuronal morphology. In neurobiology, DPYSL3 is crucial for axon guidance, neuronal differentiation, and synaptic plasticity. Its dysregulation is implicated in neurodevelopmental disorders and neurodegenerative diseases. In cancer research, DPYSL3 may contribute to tumor invasion and metastasis. Investigating DPYSL3 provides insights into neurodevelopment and neurological disorders, offering potential applications in understanding and treating conditions related to aberrant neurite outgrowth. Additionally, exploring its role in cancer may unveil new targets for cancer therapy and metastasis prevention.

Target Background

Function

Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, neuronal growth cone collapse and cell migration.

Gene References into Functions

Data indicate that CRMP4a and CRMP4b isoforms have opposite effects on cell proliferation, migration, and invasion in gastric cancer. CRMP4a expression was reduced in gastric carcinoma tissues and cell lines and seems to act as a tumor suppressor in gastric carcinoma. CRMP4b expression was higher in tumor tissue and cell lines. PMID: 27475326
DPYSL3 expression levels inversely correlated with those of VEGF and FAK. Down-regulation of DPYSL3 expression in HCC tissues may serve as a predictive biomarker for HCC after curative resection. PMID: 25173447
Expression status of DPYSL3 in gastric cancer tissues may represent a promising biomarker for the malignant behavior of GC. PMID: 25096402
DPYSL3 as pancreatic ductal adenocarcinoma-associated molecule that regulates cell adhesion and migration by stabilization of focal adhesion complex PMID: 24339867
structures provide further insight into the effects of medically relevant mutations of the DPYSL-3 gene encoding CRMP-4 and the putative enzymatic activities of CRMPs PMID: 24914979
High collapsin response mediator protein 4 expression is associated with liver metastasis in pancreatic cancer. PMID: 22805864
Downregulation of MYCN by small interfering RNA (siRNA) increased DPYSL3 levels, while upregulation of MYCN in non-MYCN NB cells decreased DPYSL3 levels. PMID: 24011394
study found the association between the rs147541241 variant and amyotrophic lateral scerosis was limited to the French population PMID: 23568759
Ulip1 was downregulated and HIF1a was upregulated in hypoxic neuroblastoma cells. PMID: 20977846
CRMP4 is a key physiological substrate of GSK3 in regulating chromosomal alignment and mitotic progression through its effect on spindle microtubules PMID: 21179545
Data show that two genes, LTB4DH and DPYSL3, were confirmed to be candidate genes for the predictor of a good immune response. PMID: 20804502
new function of CRMP4 as a metastasis-suppressor in lymph node metastasis of prostate cancer PMID: 20543870
HUlip was expressed in late fetal and early postnatal brains in the neurons of the brain stem, basal ganglia/thalamus, and dentate gyrus of the hippocampus. HUlip expression was easily detected in tumor cells undergoing neuronal differentiation. [Ulip] PMID: 15933812

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Subcellular Location

Cytoplasm. Cell projection, growth cone.

Protein Families

Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family

Tissue Specificity

Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal cord.

Database Links

HGNC: 3015

OMIM: 601168

KEGG: hsa:1809

STRING: 9606.ENSP00000343690

UniGene: Hs.519659

Code	CSB-EP622756HUa0
Abbreviation	Recombinant Human DPYSL3 protein
MSDS	MSDS Datasheet
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Dihydropyrimidinase-related protein 3 (DPYSL3)

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