Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

DLGAP5

Uniprot No.

Q15398

Research Area

Cancer

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-846aa

Target Protein Sequence

MSSSHFASRHRKDISTEMIRTKIAHRKSLSQKENRHKEYERNRHFGLKDVNIPTLEGRILVELDETSQGLVPEKTNVKPRAMKTILGDQRKQMLQKYKEEKQLQKLKEQREKAKRGIFKVGRYRPDMPCFLLSNQNAVKAEPKKAIPSSVRITRSKAKDQMEQTKIDNESDVRAIRPGPRQTSEKKVSDKEKKVVQPVMPTSLRMTRSATQAAKQVPRTVSSTTARKPVTRAANENEPEGKVPSKGRPAKNVETKPDKGISCKVDSEENTLNSQTNATSGMNPDGVLSKMENLPEINTAKIKGKNSFAPKDFMFQPLDGLKTYQVTPMTPRSANAFLTPSYTWTPLKTEVDESQATKEILAQKCKTYSTKTIQQDSNKLPCPLGPLTVWHEEHVLNKNEATTKNLNGLPIKEVPSLERNEGRIAQPHHGVPYFRNILQSETEKLTSHCFEWDRKLELDIPDDAKDLIRTAVGQTRLLMKERFKQFEGLVDDCEYKRGIKETTCTDLDGFWDMVSFQIEDVIHKFNNLIKLEESGWQVNNNMNHNMNKNVFRKKVVSGIASKPKQDDAGRIAARNRLAAIKNAMRERIRQEECAETAVSVIPKEVDKIVFDAGFFRVESPVKLFSGLSVSSEGPSQRLGTPKSVNKAVSQSRNEMGIPQQTTSPENAGPQNTKSEHVKKTLFLSIPESRSSIEDAQCPGLPDLIEENHVVNKTDLKVDCLSSERMSLPLLAGGVADDINTNKKEGISDVVEGMELNSSITSQDVLMSSPEKNTASQNSILEEGETKISQSELFDNKSLTTECHLLDSPGLNCSNPFTQLERRHQEHARHISFGGNLITFSPLQPGEF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

121.8 kDa

Protein Length

Full Length

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The generation of the recombinant Human DLGAP5 protein involves manipulating gene expression in e.coli cells. A specific DNA sequence coding for the Human DLGAP5 protein (1-846aa) is inserted to an expression vector, which is then introduced into e.coli cells. The cells that successfully receive the vector are selected and cultured to induce the expression of the desired protein. A N-terminal GST tag is attached to the protein. The recombinant Human DLGAP5 protein undergoes affinity purification, achieving a purity level exceeding 85%, as verified through SDS-PAGE analysis.

Target Background

Function

Potential cell cycle regulator that may play a role in carcinogenesis of cancer cells. Mitotic phosphoprotein regulated by the ubiquitin-proteasome pathway. Key regulator of adherens junction integrity and differentiation that may be involved in CDH1-mediated adhesion and signaling in epithelial cells.

Gene References into Functions

The bioinformatic analysis suggests that HURP promotes carcinogenesis in multiple manners. Taken together, we revealed the prognostic value of HURP in nonsmall cell lung cancer (NSCLC)patients and HURP may be a potential therapeutic target for NSCLC. PMID: 29484418
Data provide evidence for the involvement of HURP protein in the modulation of prostate cancer cell resistance to gamma irradiation via mechanism mediated by the ubiquitination of ATM and p53. PMID: 26505164
Aurora-A/HURP relays cell transforming signal to NF-kappaB, and the HURP/NF-kappaB complex is engaged in the regulation of cyclin E1 expression. PMID: 25289861
Detection of urinary HURP RNA is a useful for early detection of bladder cancer and bilharzial bladder cancer. PMID: 24375315
expression of DLG7, a hypoxia-controlled gene, holds prognostic potential in high-risk CaP; this also demonstrates that variation of oxygen tension may constitute a tool for identification of novel biomarkers for CaP. PMID: 24349376
The expression of DLGAP5 is regulated by methylation; the up-regulation of DLGAP5 contributes to hepatocellular carcinoma tumorigenesis by promoting cell proliferation. PMID: 24324629
RNA purification using functionalized MNPs was 97%. CONCLUSIONS: The developed colorimetric HURP RNA AuNP assay is sensitive, simple, and can aid noninvasive diagnosis of bladder cancer PMID: 24183881
HURP is a substrate of Aurora kinase A, which plays a crucial role in the stabilization of kinetochore fiber PMID: 23610398
Knockdown of HURP inhibits the proliferation of hepacellular carcinoma cells via downregulation of gankyrin and accumulation of p53. PMID: 22230478
Hepatocellular carcinoma patients can be separated into three prognosis-distinguishable groups by use of a risk score that is based upon HURP expression. PMID: 22022601
HURP regulates Kif18A localization and dynamics at the plus end of kinetochore microtubules. PMID: 21924616
DLGAP5-PINK1 and BUB1B-PINK1 were strong predictors of disease-free survival and overall survival, respectively, among adult patients with ACT. PMID: 22048964
results strongly suggest that HURP is a potential novel cell cycle regulator that may play a role in the carcinogenesis of human cancer cells PMID: 12527899
Cdk1-cyclin B-phosphorylated hepatoma up-regulated protein (HURP) proteolysis is regulated by Fbx7 and the SCF complex PMID: 15145941
KIAA0008 expression is associated with invasiveness of HCC; overexpression of KIAA0008 leads to a more invasive phenotype of HCC cell lines. PMID: 15340842
HURP is a potential oncogenic target of Aurora-A. PMID: 15987997
HURP controls spindle stability and dynamics to achieve efficient kinetochore capture at prometaphase, timely chromosome congression to the metaphase plate, and proper interkinetochore tension for anaphase initiation. PMID: 16769820
Dlg7 has a role in stem cell survival, in maintaining stem cell properties, and in carcinogenesis. PMID: 17322106
Phosphorylation of HURP by Aurora A provides a regulatory mechanism for the control of spindle assembly and function. PMID: 18321990
Combined expression of DLG7 and PINK1 was the best predictor of disease free survival in malignant adrenal cortex neoplasms. PMID: 19139432

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Subcellular Location

Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Note=Localizes to the spindle in mitotic cells. Colocalizes with CDH1 at sites of cell-cell contact in intestinal epithelial cells.

Protein Families

SAPAP family

Tissue Specificity

Abundantly expressed in fetal liver. Expressed at lower levels in bone marrow, testis, colon, and placenta.

Database Links

HGNC: 16864

OMIM: 617859

KEGG: hsa:9787

STRING: 9606.ENSP00000247191

UniGene: Hs.77695

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Code	CSB-EP622997HU(A4)
Abbreviation	Recombinant Human DLGAP5 protein
MSDS	MSDS Datasheet
Size	$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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