Recombinant Human E3 ubiquitin-protein ligase RBBP6 (RBBP6), partial

Code CSB-YP768776HU
MSDS
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Source Yeast
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Code CSB-EP768776HU
MSDS
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Source E.coli
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Code CSB-EP768776HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768776HU
MSDS
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Source Baculovirus
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Code CSB-MP768776HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RBBP6
Uniprot No.
Alternative Names
DKFZp686P0638; DKFZp761B2423; E3 ubiquitin-protein ligase RBBP6; MY038; P2P R; P2PR; p53 associated cellular protein of testis; p53-associated cellular protein of testis; p53-associated cellular protein, testis-derived; PACT; Proliferation potential related protein; Proliferation potential-related protein; Protein P2P R; Protein P2P-R; Protein P2PR; Protein RBQ 1; Protein RBQ1; RB binding protein 6, ubiquitin ligase; RB binding Q protein 1; RBBP 6; Rbbp6; RBBP6_HUMAN; RBQ 1; RBQ-1; RBQ1; Retinoblastoma binding protein 6 isoform 3; Retinoblastoma binding Q protein 1; Retinoblastoma-binding protein 6; Retinoblastoma-binding Q protein 1; SNAMA; SNAMA, Drosophila, homolog of
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase which promotes ubiquitination of YBX1, leading to its degradation by the proteasome. May play a role as a scaffold protein to promote the assembly of the p53/TP53-MDM2 complex, resulting in increase of MDM2-mediated ubiquitination and degradation of p53/TP53; may function as negative regulator of p53/TP53, leading to both apoptosis and cell growth. Regulates DNA-replication and the stability of chromosomal common fragile sites (CFSs) in a ZBTB38- and MCM10-dependent manner. Controls ZBTB38 protein stability and abundance via ubiquitination and proteasomal degradation, and ZBTB38 in turn negatively regulates the expression of MCM10 which plays an important role in DNA-replication.; (Microbial infection) restricts ebolavirus replication probably by impairing the vp30-NP interaction, and thus viral transcription.
Gene References into Functions
  1. RBBP6 might potentiate cervical carcinoma cell viability. PMID: 29501043
  2. Results from a study on gene expression variability markers in early-stage human embryos shows that RBBP6 is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
  3. RBBP6 overexpression could resist colorectal cancer cells against radiation. PMID: 29369481
  4. These results suggest that the RBBP6-3 isoform may be involved in promoting apoptosis in cancerous cells. PMID: 26905308
  5. RBBP6 is a candidate gene for myeloproliferative neoplasms (MPNs) susceptibility in a subset of pedigrees with familial MPN. PMID: 26574608
  6. High expression levels of RBBP6 and high apoptosis levels were found in HIV-associated nephropathy (HIVAN) cases. This direct association between RBBP6 expression and apoptosis levels suggests that RBBP6 play a role in HIVAN pathogenesis. PMID: 25910411
  7. gene silencing sensitises breast cancer cells to staurosporine and camptothecin-induced cell death PMID: 24703106
  8. RBBP6 prevents DNA damage by destabilizing the transcriptional repressor ZBTB38. PMID: 24726359
  9. RBBP6 gene variants are associated with glioma and contribute to glioma susceptibility PMID: 25127414
  10. implicate RBBP6 and iso3 as novel regulators of 3' processing, especially of RNAs with AU-rich 3' UTRs. PMID: 25319826
  11. these findings suggest that RBBP6 isoform 3 is a cell cycle regulator and may be deregulated in carcinogenesis PMID: 22139301
  12. AGO2, PACT and TRBP are required for the efficient functioning of Dicer in cells PMID: 22163034
  13. Taken together with the structural similarities to U-box-containing proteins, our data suggest that RBBP6 plays a role in chaperone-mediated ubiquitination and possibly in protein quality control. PMID: 22130672
  14. RBBP6 may be involved in the degradation of p53, thereby enhancing cell proliferation and inhibiting apoptosis in lung cancer. PMID: 21676486
  15. P2P-R expression may be translationally regulated by PUM2 and that P2P-R may modulate translation by influencing ribosomal protein gene expression PMID: 15617101
  16. RBBP6 has a role in mRNA processing and ubiquitin-like pathways PMID: 16396680
  17. The authors showed that RBBP6 and its isolated RING finger domain were able to ubiquitinate YB-1, resulting in its degradation in the proteosome. As a result, RBBP6 was able to suppress the levels of YB-1 in vivo and reduce its transactivational ability. PMID: 18851979

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Subcellular Location
Nucleus, nucleolus. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with mitotic chromosomes. Colocalizes with NEK6 in the centrosome.
Tissue Specificity
Highly expressed in the placenta and testis. Expressed at lower levels in the brain, heart, kidney, liver and lung. Overexpressed in esophageal cancer.
Database Links

HGNC: 9889

OMIM: 600938

KEGG: hsa:5930

STRING: 9606.ENSP00000317872

UniGene: Hs.188553

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