Recombinant Human ELL-associated factor 2 (EAF2)

Code CSB-YP856910HU
MSDS
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Source Yeast
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Code CSB-EP856910HU
MSDS
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Source E.coli
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Code CSB-EP856910HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856910HU
MSDS
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Source Baculovirus
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Code CSB-MP856910HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EAF2
Uniprot No.
Alternative Names
BM 040; BM040; EAF 2; EAF2; EAF2_HUMAN; Ehrlich S II transcriptional activator factor ; ELL associated factor 2; ELL-associated factor 2; Festa; Testosterone regulated apoptosis inducer and tumor suppressor; Testosterone regulated apoptosis inducer and tumor suppressor protein; Testosterone-regulated apoptosis inducer and tumor suppressor protein; TRAITS; U19; Uncharacterized bone marrow protein BM 040; Uncharacterized bone marrow protein BM040
Species
Homo sapiens (Human)
Expression Region
1-260
Target Protein Sequence
MNSAAGFSHL DRRERVLKLG ESFEKQPRCA FHTVRYDFKP ASIDTSSEGY LEVGEGEQVT ITLPNIEGST PPVTVFKGSK KPYLKECILI INHDTGECRL EKLSSNITVK KTRVEGSSKI QYRKEQQQQQ MWNSARTPNL VKHSPSEDKM SPASPIDDIE RELKAEASLM DQMSSCDSSS DSKSSSSSSS EDSSSDSEDE DCKSSTSDTG NCVSGHPTMT QYRIPDIDAS HNRFRDNSGL LMNTLRNDLQ LSESGSDSDD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.
Gene References into Functions
  1. Simultaneous inactivation of EAF2 and p53 can act to activate STAT3 and drive prostate tumorigenesis. PMID: 29518696
  2. The results indicated that Eaf2 was able to effectively suppress H2O2induced apoptosis of HLE cells via inhibition of caspase 3 production and activation of Wnt3a signaling. PMID: 29257273
  3. EZH2 promotes tumorigenesis and malignant progression in part by activating glycolysis through an EAF2-HIF1alpha signaling axis. PMID: 27259264
  4. Concurrent p53 nuclear staining and EAF2 downregulation was associated with high Gleason score In human prostate cancer specimens. PMID: 29029019
  5. analysis showed that EAF2 and EAF1 are required for the recruitment and retention of Ku70/Ku80 to DNA damage sites and play a functional role in nonhomologous end-joining DNA repair. Findings provide evidence for EAF2 as a key factor mediating androgen protection of DNA damage via Ku70/Ku80 in prostate cancer cells. PMID: 27721405
  6. EAF2 binding does not alter Smad3 phosphorylation but causes Smad3 cytoplasmic retention, competes with Smad4 for binding to Smad3, and prevents p300-Smad3 complex formation PMID: 26370086
  7. Findings suggest that regulation of the AR signaling pathway, cell proliferation, and migration through FOXA1 represents an important mechanism of EAF2 suppression of prostate carcinogenesis. PMID: 25808853
  8. Together these findings identify a novel physical and functional interaction between EAF2 and the Rb pathway. PMID: 24727455
  9. EAF2 bound to and suppressed HIF-1alpha. PMID: 24421387
  10. the role of the EAF2 in response to simvastatin and lovastatin in HCT-116 colon cancer cells PMID: 21483694
  11. Data suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. PMID: 19826414
  12. U19 is growth inhibitory and tumor suppressive and that the disruption of androgen-dependent growth inhibition via U19 down-regulation is commonly associated with prostate cancer progression. PMID: 12907652
  13. ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. PMID: 16006523
  14. ELL may be an important factor required for U19/Eaf2 function because U19/Eaf2 nuclear localization and transactivation activity are essential for its function as a transcription factor. PMID: 16114057
  15. FB1 is an important binding partner and a functional regulator of U19/EAF2, EAF1, and/or ELL. PMID: 17395368

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Subcellular Location
Nucleus speckle.
Protein Families
EAF family
Tissue Specificity
Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.
Database Links

HGNC: 23115

OMIM: 607659

KEGG: hsa:55840

STRING: 9606.ENSP00000273668

UniGene: Hs.477325

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