EAF2 Antibody

Datasheet

Code	CSB-PA856910LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse kidney tissue All lanes: EAF2 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 29, 15 kDa Observed band size: 45 kDa IHC image of CSB-PA856910LA01HU diluted at 1:1000 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA856910LA01HU at 1:333, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) EAF2 Polyclonal antibody

Uniprot No.

Q96CJ1

Target Names

EAF2

Alternative Names

BM 040 antibody; BM040 antibody; EAF 2 antibody; EAF2 antibody; EAF2_HUMAN antibody; Ehrlich S II transcriptional activator factor antibody; ELL associated factor 2 antibody; ELL-associated factor 2 antibody; Festa antibody; Testosterone regulated apoptosis inducer and tumor suppressor antibody; Testosterone regulated apoptosis inducer and tumor suppressor protein antibody; Testosterone-regulated apoptosis inducer and tumor suppressor protein antibody; TRAITS antibody; U19 antibody; Uncharacterized bone marrow protein BM 040 antibody; Uncharacterized bone marrow protein BM040 antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human ELL-associated factor 2 protein (6-100AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The EAF2 Antibody (Product code: CSB-PA856910LA01HU) is Non-conjugated. For EAF2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA856910LB01HU	EAF2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA856910LC01HU	EAF2 Antibody, FITC conjugated
Biotin	CSB-PA856910LD01HU	EAF2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:1000-1:2000
IF	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Acts as a transcriptional transactivator of TCEA1 elongation activity. Acts as a transcriptional transactivator of ELL and ELL2 elongation activities. Potent inducer of apoptosis in prostatic and non-prostatic cell lines. Inhibits prostate tumor growth in vivo.

Gene References into Functions

Simultaneous inactivation of EAF2 and p53 can act to activate STAT3 and drive prostate tumorigenesis. PMID: 29518696
The results indicated that Eaf2 was able to effectively suppress H2O2induced apoptosis of HLE cells via inhibition of caspase 3 production and activation of Wnt3a signaling. PMID: 29257273
EZH2 promotes tumorigenesis and malignant progression in part by activating glycolysis through an EAF2-HIF1alpha signaling axis. PMID: 27259264
Concurrent p53 nuclear staining and EAF2 downregulation was associated with high Gleason score In human prostate cancer specimens. PMID: 29029019
analysis showed that EAF2 and EAF1 are required for the recruitment and retention of Ku70/Ku80 to DNA damage sites and play a functional role in nonhomologous end-joining DNA repair. Findings provide evidence for EAF2 as a key factor mediating androgen protection of DNA damage via Ku70/Ku80 in prostate cancer cells. PMID: 27721405
EAF2 binding does not alter Smad3 phosphorylation but causes Smad3 cytoplasmic retention, competes with Smad4 for binding to Smad3, and prevents p300-Smad3 complex formation PMID: 26370086
Findings suggest that regulation of the AR signaling pathway, cell proliferation, and migration through FOXA1 represents an important mechanism of EAF2 suppression of prostate carcinogenesis. PMID: 25808853
Together these findings identify a novel physical and functional interaction between EAF2 and the Rb pathway. PMID: 24727455
EAF2 bound to and suppressed HIF-1alpha. PMID: 24421387
the role of the EAF2 in response to simvastatin and lovastatin in HCT-116 colon cancer cells PMID: 21483694
Data suggest that U19/EAF2 regulates the expression of TSP-1 via blocking p53 repression of the TSP-1 promoter. PMID: 19826414
U19 is growth inhibitory and tumor suppressive and that the disruption of androgen-dependent growth inhibition via U19 down-regulation is commonly associated with prostate cancer progression. PMID: 12907652
ELL-associated factors 1 and 2 are positive regulators of RNA polymerase II elongation factor ELL. PMID: 16006523
ELL may be an important factor required for U19/Eaf2 function because U19/Eaf2 nuclear localization and transactivation activity are essential for its function as a transcription factor. PMID: 16114057
FB1 is an important binding partner and a functional regulator of U19/EAF2, EAF1, and/or ELL. PMID: 17395368

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Subcellular Location

Nucleus speckle.

Protein Families

EAF family

Tissue Specificity

Expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, spleen, prostate, testis, small intestine, colon, adrenal, bone marrow, lymph node, spinal gland, stomach, thyroid, trachea, thymus, liver and leukocytes.

Database Links

HGNC: 23115

OMIM: 607659

KEGG: hsa:55840

STRING: 9606.ENSP00000273668

UniGene: Hs.477325

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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