Recombinant Human ETS homologous factor (EHF)

Code CSB-YP007494HU
MSDS
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Source Yeast
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Code CSB-EP007494HU
MSDS
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Source E.coli
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Code CSB-EP007494HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007494HU
MSDS
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Source Baculovirus
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Code CSB-MP007494HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EHF
Uniprot No.
Alternative Names
EHF; EHF_HUMAN; Epithelium-specific Ets transcription factor 3; ESE-3; ESE3; ESE3 transcription factor; ESE3B; ESEJ; Ets domain transcription factor; ETS domain-containing transcription factor; ETS homologous factor; hEHF
Species
Homo sapiens (Human)
Expression Region
1-300
Target Protein Sequence
MILEGGGVMN LNPGNNLLHQ PPAWTDSYST CNVSSGFFGG QWHEIHPQYW TKYQVWEWLQ HLLDTNQLDA NCIPFQEFDI NGEHLCSMSL QEFTRAAGTA GQLLYSNLQH LKWNGQCSSD LFQSTHNVIV KTEQTEPSIM NTWKDENYLY DTNYGSTVDL LDSKTFCRAQ ISMTTTSHLP VAESPDMKKE QDPPAKCHTK KHNPRGTHLW EFIRDILLNP DKNPGLIKWE DRSEGVFRFL KSEAVAQLWG KKKNNSSMTY EKLSRAMRYY YKREILERVD GRRLVYKFGK NARGWRENEN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional activator that may play a role in regulating epithelial cell differentiation and proliferation. May act as a repressor for a specific subset of ETS/AP-1-responsive genes and as a modulator of the nuclear response to mitogen-activated protein kinase signaling cascades. Binds to DNA sequences containing the consensus nucleotide core sequence GGAA. Involved in regulation of TNFRSF10B/DR5 expression through Ets-binding sequences on the TNFRSF10B/DR5 promoter. May contribute to development and carcinogenesis by acting as a tumor suppressor gene or anti-oncogene.
Gene References into Functions
  1. A negative correlation between EHF protein and miR206 expression. PMID: 28714026
  2. Human prostate tumors with IL-6 elevation and loss of ESE3/EHF were associated with STAT3 activation and displayed upregulation of genes related to cell adhesion, cancer stem-like and metastatic spread. PMID: 27732936
  3. EHF is a novel functional oncogene in thyroid cancer by transcriptionally regulating HER2 and HER3, and may represent a potential therapeutic target for this cancer PMID: 27517321
  4. The role of EHF in modifying Cystic Fibrosis lung disease severity. PMID: 28549169
  5. is a novel functional oncogene in gastric cancer by regulating the human epidermal growth factor receptor (HER) family of receptor tyrosine kinases. PMID: 27787520
  6. tight control by ESE3/EHF over the Lin28/let-7 axis is a critical barrier to malignant transformation. PMID: 27197175
  7. ESE3 as a negative regulator of pancreatic ductal adenocarcinomas (PDAC) progression and metastasis by enforcing E-cadherin upregulation. PMID: 27923832
  8. EHF activates expression of the SAM pointed domain-containing ETS transcription factor, which contributes to goblet cell hyperplasia. PMID: 28461336
  9. Results found that EHF was elevated in ovarian cancer tissues and correlated with short survival. Its overexpression in ovarian cancer cells promoted cell growth and metastasis, and inhibited cell apoptosis. PMID: 26258986
  10. This study suggests that ESE3 plays an important role in the carcinogenesis of Esophageal Squamous Cell Carcinoma through changes in subcellular localization and may act as a tumor suppressor gene in Esophageal Squamous Cell Carcinoma. PMID: 25950810
  11. EHF modifies the CF phenotype by altering capabilities of the epithelial cell to correctly process the folding and trafficking of mutant p.Phe508del-CFTR. PMID: 24105369
  12. Results suggest PPAR-gamma to be involved in the regulation of ESE-3b expression during monocyte-derived dendritic cells (moDCs) development and that ESE-3 expression is not correlated with the immunogenicity of dendritic cells (DCs). PMID: 24219556
  13. The SRC family tyrosine kinase HCK and the ETS family transcription factors SPIB and EHF regulate transcytosis across a human follicle-associated epithelium model. PMID: 23439650
  14. ESE-3b is the functionally most important ESE-3 isoform in dendritic cells PMID: 23185370
  15. Low ESE3/EHF expression was also associated with increased biochemical recurrence of prostate cancer and reduced overall survival after prostatectomy PMID: 22505649
  16. EHF mRNA levels differentiate ovarian carcinoma from malignant mesothelioma. EHF may be a novel prognostic marker in ovarian carcinoma. PMID: 21855111
  17. These results indicate that EHF-mediated RUVBL1 expression allows colon tumour cells to avoid p53-mediated apoptosis. PMID: 21617703
  18. Overexpression of ESE-3 protein in human bronchial smooth muscle cells inhibits MMP-1 promoter activity, suggesting that ESE-3 may function as a transcriptional repressor. PMID: 12444029
  19. In addition, we show the possible involvement of co-factors CBP and p300 in ESE-3-mediated DR-5 up-regulation. PMID: 17027647
  20. Aberrant expression of EHF and the subsequent disruption of p27-mediated senescence and telomerase activity is likely to contribute significantly to tumor progression. EHF might be a promising target for future cancer therapeutics. PMID: 17172423
  21. ESE-3 plays a role in the induction of cellular senescence as a downstream molecule of stress-induced mitogen-activated protein kinase p38. PMID: 17627613
  22. Implicated as a candidate tumor suppressor in prostate cancer; decreased expression may result in loss of important regulatory mechanisms in prostate epithelial cells. PMID: 18037958
  23. ESE-1 and ESE-3 play an important role in airway inflammation. PMID: 18475289

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Subcellular Location
Nucleus.
Protein Families
ETS family
Tissue Specificity
Expressed exclusively in tissues with a high content of epithelial cells. Highly expressed in salivary gland, mammary gland, prostate, and lung. Weakly expressed in kidney and colon. Not detected in heart, brain, placenta, liver, skeletal muscle, spleen,
Database Links

HGNC: 3246

OMIM: 605439

KEGG: hsa:26298

STRING: 9606.ENSP00000435835

UniGene: Hs.653859

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