Recombinant Human Early endosome antigen 1 (EEA1), partial

Code CSB-YP007405HU
MSDS
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Source Yeast
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Code CSB-EP007405HU
MSDS
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Source E.coli
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Code CSB-EP007405HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007405HU
MSDS
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Source Baculovirus
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Code CSB-MP007405HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EEA1
Uniprot No.
Alternative Names
Early endosome antigen 1; Early endosome antigen 1; 162kD; Early endosome associated protein; EEA 1; EEA1; EEA1_HUMAN; Endosome associated protein p162; Endosome-associated protein p162; MST105; MSTP105; ZFYVE2; Zinc finger FYVE domain containing protein 2; Zinc finger FYVE domain-containing protein 2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking.
Gene References into Functions
  1. This also suggests that this pathway is involved in EEA1-vesicles biogenesis. PMID: 30188634
  2. identification of allergic bronchopulmonary aspergillosis -associated variants in EEA that have functional effects on macrophage phagocytosis and phagolysosome acidification of A. fumigatus conidia. PMID: 29547649
  3. results suggest a new mechanism in which Rab5 induces a change in flexibility of EEA1, generating an entropic collapse force that pulls the captured vesicle towards the target membrane to initiate docking and membrane fusion PMID: 27556945
  4. EEA1 mobility on endosomes is decreased upon stimulation of EGF receptor endocytosis in HeLa cells PMID: 26993163
  5. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized TbetaRI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). PMID: 25998683
  6. In serum-deprivated HeLa cells with low endocytic activity there two types of EEA1-vesicles: the first one carries the both EEA1 and Rab5 at high levels; the second consists of weakly decorated EEA1-vesicles that can be both Rab5-positive and -negative. PMID: 25711083
  7. Data indicate that early endosome antigen 1 (EEA1) is important for the small GTPase Rab31-mediated enhancement of ligand-bound EGF receptor (EGFR) endocytic trafficking. PMID: 24644286
  8. nuclear uptake of Abeta involves the dynamin-dependent EEA1 and TGF-beta/Smad signaling pathways. PMID: 24491918
  9. We therefore propose that the N1072K variant of the EEA1 gene is a candidate mutation for susceptibility to diabetes in the Japanese population. PMID: 23499280
  10. EEA1 interacts with Phafin2 and colocolizes with Phafin2 in endosome membrane. PMID: 22816767
  11. NMR analyses of the interaction between the FYVE domain of early endosome antigen 1 (EEA1) and phosphoinositide embedded in a lipid bilayer. PMID: 22915584
  12. p97 inhibition causes increased EEA1 self-association at the endosome membrane. PMID: 21556036
  13. The knockdown of STEAP4 inhibits insulin-stimulated glucose transport and GLUT4 translocation via the attenuated phosphorylation of Akt, independent of the effects of EEA1. PMID: 21468601
  14. crystal structure of Rab5A in complex with the EEA1 C(2)H(2) zinc finger PMID: 20534488
  15. activation of p38 MAPK caused a decrease in EEA1 colocalization with phagosomes, arresting maturation into the phagolysosome PMID: 12963735
  16. macropinosomes do not mature to late endosomes or fuse with lysosomes. EEA1 continuously mediates homotypic fusion as long as the macropinosomes persist. PMID: 15052657
  17. These results suggest that EEA1 is involved in neuronal synaptic vesicle function and axonal transport and growth. EEA1 may undergo calcium-dependent conformational changes that are required for binding to SNAP-25. PMID: 15451443
  18. Results describe the geometry of the micelle penetration of the early endosome antigen 1 FYVE domain. PMID: 16331966
  19. PIKfyve is distributed in microdomains that are distinct from those occupied by EEA1 and Hrs PMID: 16448788
  20. Impairment of internalization of specific glutamate receptors and their subsequent accumulation in the synapse may account for the neurological deficits observed in some patients developing EEA1 autoantibodies. PMID: 17113235
  21. results suggest that the local production of PtdIns(3)P implicates the fusion of macropinosomes via EEA1 as well as conventional early endosomes PMID: 17146146

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Subcellular Location
Cytoplasm. Early endosome membrane; Peripheral membrane protein.
Database Links

HGNC: 3185

OMIM: 605070

KEGG: hsa:8411

STRING: 9606.ENSP00000317955

UniGene: Hs.567367

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