Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

EEF1D

Uniprot No.

P29692

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

Antigen NY CO 4; Antigen NY-CO-4; EEF 1D; EEF1D; EF 1 delta; EF 1D; EF-1-delta; EF1D; EF1D_HUMAN; Elongation factor 1 delta; Elongation factor 1-delta; eukaryotic translation elongation factor 1 delta (guanine nucleotide exchange protein); Eukaryotic translation elongation factor 1 delta; FP1047; Guanine nucleotide exchange protein

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-281aa

Target Protein Sequence

ATNFLAHEKIWFDKFKYDDAERRFYEQMNGPVAGASRQENGASVILRDIARARENIQKSLAGSSGPGASSGTSGDHGELVVRIASLEVENQSLRGVVQELQQAISKLEARLNVLEKSSPGHRATAPQTQHVSPMRQVEPPAKKPATPAEDDEDDDIDLFGSDNEEEDKEAAQLREERLRQYAEKKAKKPALVAKSSILLDVKPWDDETDMAQLEACVRSIQLDGLVWGASKLVPVGYGIRKLQIQCVVEDDKVGTDLLEEEITKFEEHVQSVDIAAFNKI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

35.0kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Human EF1D covers amino acids 2-281. The calculated molecular weight for this EF1D protein is 35 kDa. This EF1D recombinant protein is manufactured in e.coli. The EF1D gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant EF1D protein during the following stages.

Human elongation factor 1-delta (EEF1D) is a protein involved in protein synthesis, playing a crucial role in the elongation phase of translation. It is a subunit of the eukaryotic elongation factor 1 complex, which delivers aminoacyl-tRNA to the ribosome during mRNA translation. EEF1D specifically interacts with aminoacyl-tRNA and facilitates the accurate and efficient addition of amino acids to the growing polypeptide chain. Beyond its primary role in translation, EEF1D has been implicated in diverse cellular processes, including cytoskeletal organization, apoptosis, and regulation of gene expression. Its multifunctional nature highlights its significance in maintaining cellular homeostasis and suggests potential implications in various cellular pathways.

Target Background

Function

EF-1-beta and EF-1-delta stimulate the exchange of GDP bound to EF-1-alpha to GTP, regenerating EF-1-alpha for another round of transfer of aminoacyl-tRNAs to the ribosome.; Regulates induction of heat-shock-responsive genes through association with heat shock transcription factors and direct DNA-binding at heat shock promoter elements (HSE).

Gene References into Functions

eukaryotic translation elongation factor 1delta knockdown in oral squamous cell carcinoma reduced cell proliferation and induced EMT (epithelial-mesenchymal transition) phenotypes PMID: 26823560
Taken together, our data suggest EFF1D functions as a novel negative regulator of SIAH-1 PMID: 21633900
overexpression is positively correlated with malignant transformation of 16HBE cells induced by CdC12, but is not correlated with DNA mutations PMID: 20514991
These results further confirm that overexpression of TEF-1 delta is oncogenic and the antisense TEF-1 delta mRNA expression reverses its oncogenic potential. PMID: 12210501
Interaction between HrHRF and EF-1delta taken with some of the recently published information concerning the TCTP (HrHRF) mentioned above suggest a possible intracellular role for TCTP/HrHRF. PMID: 15062873
Increased EF-1 delta mRNA expression is associated with lymph node metastases and advanced disease stages in esophageal carcinoma PMID: 15199388
Gain of 8q-mapped EF-1 delta is associated with adverse outcome in Medulloblastoma. PMID: 16968546
Repression of deltaEF1 plays a key role in mediating BMP-6-induced transcriptional activation of E-cadherin in breast cancer cells. PMID: 17997862

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Subcellular Location

[Isoform 2]: Nucleus.

Protein Families

EF-1-beta/EF-1-delta family

Tissue Specificity

Isoform 2 is specifically expressed in brain, cerebellum and testis.

Database Links

HGNC: 3211

OMIM: 130592

KEGG: hsa:1936

STRING: 9606.ENSP00000391944

UniGene: Hs.333388

Code	CSB-EP007431HU
Abbreviation	Recombinant Human EF1D protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Elongation factor 1-delta (EEF1D)

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