Recombinant Human Ephrin-B2(EFNB2),partial

Code CSB-YP007466HU1
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Source Yeast
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Code CSB-EP007466HU1
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Source E.coli
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Code CSB-EP007466HU1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007466HU1
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Source Baculovirus
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Code CSB-MP007466HU1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EFNB2
Uniprot No. P52799
Alternative Names EFN B2; EFNB 2; Efnb2; EFNB2_HUMAN; Eph related receptor tyrosine kinase ligand 5; EPH-related receptor tyrosine kinase ligand 5; ephrin B2; Ephrin-B2; EphrinB2; EPLG 5; EPLG5; Htk L; HTK ligand; HTK-L; HTKL; LERK 5; LERK-5; LERK5; Ligand of eph related kinase 5; MGC126226; MGC126227; MGC126228; OTTMUSP00000024973
Species Homo sapiens (Human)
Protein Length Extracellular domain
Tag Info The following tags are available.
N-terminal His-tagged
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds to receptor tyrosine kinase including EPHA4, EPHA3 and EPHB4. Together with EPHB4 plays a central role in heart morphogenesis and angiogenesis through regulation of cell adhesion and cell migration. EPHB4-mediated forward signaling controls cellular repulsion and segregation from EFNB2-expressing cells. May play a role in constraining the orientation of longitudinally projecting axons.
Gene References into Functions
  1. This is the novel report describing to demonstrate that the Runx2 expression of MSC is synergistically influenced by the hydrogels elasticity and their manner of ephrinB2 immobilized. PMID: 28300720
  2. the binding of the viral attachment protein G to its host receptor ephrinB2 was studied and showed monomeric and dimeric receptors activate distinct conformational changes in G. PMID: 28974687
  3. results suggest that ephrin-B2 overexpression and activation of the ephrin-B2 reverse signaling pathway in tumor microenvironment in OSCC facilitates progression and lymph node metastasis via enhancement of malignant potential and interaction with surrounding cells PMID: 29190834
  4. Proteomic analysis of their secretome reveals a signature, including ephrin-B2, that predicts decreased survival of glioma patients. We find that ephrin-B2 is an important pro-angiogenic tenascin-C effector. PMID: 27926865
  5. In human glioblastoma stem-like cells-derived orthotopic xenografts, EFNB2 knock-down blocked tumour initiation and treatment of established tumours with ephrin-B2-blocking antibodies suppressed progression. PMID: 27350048
  6. roles in stabilizing vascular-like structures generated by stem cells from apical papilla (SCAPs) and umbilical vein endothelial cells (HUVECs) PMID: 27451120
  7. High EFNB2 expression is associated with preeclampsia. PMID: 27069008
  8. Inhibition of EphB4 forward signaling using soluble EphB4 protein fused with murine serum albumin failed to affect eRMS model tumor progression, but did moderately slow progression in murine aRMS PMID: 28817624
  9. Study shows that EFNB2 is overexpressed in head and neck squamous cell carcinoma, pancreatic adenocarcinoma, and urothelial bladder carcinoma, and its overexpression correlates with poor survival of the cancer patients. PMID: 27649287
  10. Five SNPs in the 3' region of the EFNB2 were in linkage disequilibrium and significantly associated with hypertension for male but not female subjects. PMID: 27530629
  11. These results indicate a novel mechanism of ephrin-Eph signaling independent of direct cell contact and proteolytic cleavage and suggest the participation of EphB2(+)extracellular vesicles in neural development and synapse physiology. PMID: 27354374
  12. Association of EFNB2 rs9520087 with symptoms of schizophrenia in Chinese Zhuang and Han populations. PMID: 27028544
  13. miR-137 directly down-regulates the expression of EFNB2, and a genetic variant in the RNA binding site in EFNB2 gene affects the expression regulation. MiR-137, as a risk factor for schizophrenia PMID: 27650867
  14. 5-fluorouracil-induced ephrin-B2 reverse signaling promotes tumorigenesis through the Src-ERK pathway, and drives EMT via the Src-FAK pathway. PMID: 26494468
  15. Together, these findings suggest that CCL2, RARRES2 and EFNB2 are host cell factors involved in respiratory syncytial virus replication. PMID: 26277777
  16. These genes may be potential biomarkers for identification of subclinical lymph node involvement in papillary thyroid carcinoma (PTC) PMID: 24634162
  17. increased notably in preeclampsia endothelial progenitor cells and placenta PMID: 26212082
  18. Results suggest a potential signalling hierarchy between Delta-like 1 and ephrin-B2 ligands, as neural stem cells adopt the Delta-like 1 phenotype of stem cell maintenance on simultaneous presentation of both signals. PMID: 26754526
  19. role in maternal spiral artery remodeling in first trimester PMID: 25748802
  20. EfnB2 is an essential regulator of endothelial cell death and vessel pruning. This regulation depends upon phosphotyrosine-EfnB2 signaling repressing JNK3 activity via STAT1. PMID: 25807892
  21. Results suggest that ephrinB2 may function as a colorectal cancer cells growth stimulator. PMID: 25434750
  22. Molecular recognition of human ephrinB2 cell surface receptor by an emergent African henipavirus. PMID: 25825759
  23. Data suggest that fusion of Nipah viruses with host cells is facilitated by two of viral membrane proteins, the G protein and the F protein; G head domain binds to human ephrins B2 and B3 altering conformational density of entire G head domain. PMID: 24615845
  24. Human ephrin-B2 is poorly cleaved by KLK4 while the homologous mouse is not. PMID: 25724897
  25. Downregulation of the endothelial genes Notch1 and ephrinB2 in patients with nodular regenerative hyperplasia. PMID: 23870033
  26. EphB4 and ephrin B2 play important roles in neovascularization and arteriovenous differentiation/plasticity. PMID: 24517927
  27. Despite the negative results of our mutation screening, we still consider EFNB2 an excellent candidate gene for contributing to the development of Anorectal malformations in humans. PMID: 24038947
  28. EphB2 and Ephrin-B2 interactions mediate human mesenchymal stem cell suppression of activated T-cells. PMID: 23711177
  29. NNMT expression regulates neurone morphology in vitro via the sequential activation of the EFNB2 and Akt cellular signalling pathways. PMID: 23764850
  30. High levels of ephrinB2 over-expression increases the osteogenic differentiation of human mesenchymal stem cells PMID: 23201622
  31. EphrinB2 is an important indicator of poorly differentiated hepatocarcinoma. PMID: 22024229
  32. Specific Ab-based ephrinB2 targeting may represent an effective therapeutic strategy to be used as an alternative or in combination with existing antiangiogenic drugs for treating patients with cancer and other angiogenesis-related diseases. PMID: 22446484
  33. Monoclonal antibody developed in this study may be used as a reagent to probe ephrin-B2 distribution in normal as well as in pathological conditions and to antagonize ephrin-B2 interaction with EphB4 for basic science and therapeutic applications. PMID: 22292016
  34. The expressions of ephrinB2 and EphB4 were significantly higher in NSCLC tissue than in control tissues, and were positively correlated with lymphatic metastasis. PMID: 21575518
  35. deregulated ephrin-B2 expression interferes with the regulation of the stem cell niche and leads to a shift of the differentiation pathway and may thereby contribute to the acquisition of the metastatic phenotype PMID: 22020958
  36. ephrin-B2-EphB4 signaling between PDLF and osteoblasts of the alveolar bone might contribute to osteogenesis at tension sites during orthodontic tooth movement PMID: 21880727
  37. Results indicated that the G-H loop of ephrin-B2 was indeed critical for the interaction between ephrin-B2 and Nipah virus-G. PMID: 21632558
  38. EphB4/ephrinB2 interactions between tumor cells and endothelial cells identify a mechanism for site-specific metastatic dissemination of tumor cells. PMID: 21047731
  39. High ephrin-B2 is associated with malignant urogenital tissue. PMID: 19272799
  40. The find of this study indicated that EFNB2 gene may be a candidate susceptibility gene for schizophrenia in the Han Chinese population. PMID: 20483485
  41. The data indicate that MSCs expressing Ephrin-B2 represent a novel proangiogenic cell source to promote neovascularization in ischemic tissues. PMID: 20491587
  42. Ephrin-B2 is a potent regulator of endothelial cell behavior, and indicate that the control of cell migration and angiogenesis by ephrins might involve both receptor-dependent and receptor-independent activities. PMID: 20233847
  43. The crystal structure of the EphA4 ligand-binding domain in complex with ephrin-B2, is reported. PMID: 19875447
  44. ephrin-B2 plays a critical role in glioma invasion PMID: 19728339
  45. expressed differentially in colon carcinoma and normal mucosa specimens and may play a role in progression of colon carcinoma PMID: 11920461
  46. Endogenous ephrin B2 from human umbilical artery endothelial cells activates a kinase that phosphorylates murine GST-ephrin-B1 cytoplasmic domain fusion proteins. PMID: 11983165
  47. role of ephrin-B2 in erythropoiesis PMID: 12051776
  48. The entire cytoplasmic domain of ephrin-B2 and its N-terminal fragment, residues 253-300, lack the ability to fold into a well-defined three dimensional structure and are particularly prone to aggregation. PMID: 12206665
  49. These data identify distinct propulsive and repulsive effector functions of endothelial ephrinB2 and EphB4 that mediate spatial positional signals during angiogenesis and vessel assembly. PMID: 12734395
  50. ephrin B2 is present in human retinal endothelial cells and vascular growth may be modulated in the retina through activation of the PI3K pathway. PMID: 14499344
  51. Overexpression of ephrin-B2 suppresses tumour cell growth and vascular function in an in vivo colon cancer model PMID: 15083195
  52. in hyperinflammatory lesions, ephrinB2 was predominantly expressed in macrophage-like cells and EphB4 in small venules; syntenin and syndecan-1 were up-regulated in EphB4-positive endothelial cells after stimulation with preclustered ephrinB2 PMID: 15126321
  53. EphB4 and ephrin-B2 molecules, which have specific expression patterns during artery and vein development, respectively, were expressed in the placenta PMID: 15193868
  54. Ephrin B2 is an important novel factor in Kaposi sarcoma biology and a potential target for therapy. PMID: 15471957
  55. defined the expression patterns of human ephrinB2, EphB4, and EphB2 in normal vessels of neonates (i.e. umbilici) and adults and compared them with those in congenital venous malformations PMID: 15718372
  56. analysis of binding between the Tyr-phosphorylated human ephrinB2 and Nck2 SH2 domain PMID: 15764601
  57. ephrin-B signaling might represent a novel protective mechanism that promotes intestinal epithelial wound healing, with potential impact on epithelial restitution in Crohn disease. PMID: 15996027
  58. ephrin-B2 was found capable of rendering HeLa-USU cells permissive for Hendra virus and Nipah virus-mediated cell fusion as well as infection by live virus. PMID: 15998730
  59. ephrinB2 is a functional receptor for Nipah virus PMID: 16007075
  60. EphB4 and ephrin-B2 are instrumental in the establishment of a functional placental structure and of the utero-placental circulation. PMID: 16343615
  61. endothelial cell ephrinB2 expression is controlled by microenvironmental determinants rather than being an intrinsic endothelial cell differentiation marker PMID: 16357318
  62. Dll4 stimulation but not Jagged1 markedly induced ephrinB2 expression PMID: 16430858
  63. The results provide critical information on the EphB4*ephrinB2 protein interfaces and their mode of interaction, which will facilitate development of small molecule compounds inhibiting the EphB4*ephrinB2 interaction as novel cancer therapeutics. PMID: 16867992
  64. indicate that stimulated lymphocytes express EphrinB2, which has the potential to activate endothelial cells PMID: 17123494
  65. High ephrinB2 is associated with carcinogenesis and portal vein tumor thrombus formation in human hepatocellular PMID: 17167981
  66. demonstrates that Eph B4 and Ephrin B2 interaction may play an important role in the oncogenesis and angiogenesis of cervical carcinomas PMID: 17196593
  67. Lubrol-RAFTs probably represent the platform for tumor-promoting ephrin-B2-integrin-beta1 interaction, which could become an interesting target for future antitumoral therapies PMID: 17380111
  68. EFNB2 gene expression may be a biological marker and a useful prognostic indicator in patients with oesophageal squamous cell carcinoma. PMID: 17611172
  69. Overexpression of ephrinB2 is associated with tumour advancement of ovarian cancers PMID: 18231102
  70. The ephrin-B2 ectodomain binds to EphB2 with a K(d) of 22.3 nM to form a tight complex in which the tip of the C-D loop and the C-terminus still remain largely flexible. PMID: 18300229
  71. expression of ephrinB2 in adult endothelial cells is up-regulated during arterial remodeling and controlled by cyclic stretch PMID: 18445690
  72. crystal structures of both Nipah and Hendra attachment glycoproteins in complex with human EFNB2 PMID: 18488039
  73. Platelet-derived growth factor signaling through ephrin-b2 regulates hepatic vascular structure and function. PMID: 18570897
  74. polymorphisms in the EFNB2 gene do not appear to contribute in a substantial way to non-diabetic, diabetic or all-cause ESRD susceptibility in African-Americans PMID: 18580054
  75. MMP7 and MMP9-mediated cleavage of EphB2 is induced by receptor-ligand interactions at the cell surface and that this event triggers cell-repulsive responses PMID: 18713744
  76. We demonstrated limited Eph/Ephrin expression; notably, the only ligand highly expressed is EphrinB2 in Kaposi sarcoma PMID: 18836096
  77. EphBR-ephrinB interactions lead to monocyte adhesion and transmigration through the vascular endothelium. PMID: 18957513
  78. EphB4 receptor activation by ephrin B2 in osteoarthritis subchondral bone could affect abnormal metabolism in this tissue by inhibiting resorption factors and their activities PMID: 19035475
  79. EB2 is not only necessary but also sufficient to allow the establishment of a productive NiV infection. PMID: 19036148
  80. Ephrin-B2 signaling influence cell-to cell fusion and virus entry by the ratio between the NiV envelope glycoproteins and surface receptors. PMID: 19108727
  81. Localization of EphnB2 is dominantly in the cancer cells of uterine cervical cancers of all cases given. PMID: 19356789
  82. Our study is the first to provide data on the presence and role of ephrin B2/EphB4 receptors in human chondrocytes/cartilage PMID: 19664212

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Ephrin family
Tissue Specificity Lung and kidney.
Database Links

HGNC: 3227

OMIM: 600527

KEGG: hsa:1948

STRING: 9606.ENSP00000245323

UniGene: Hs.149239

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