Recombinant Human Eukaryotic translation initiation factor 4E type 3 (EIF4E3)

Code CSB-YP822710HU
MSDS
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Source Yeast
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Code CSB-EP822710HU
MSDS
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Source E.coli
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Code CSB-EP822710HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP822710HU
MSDS
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Source Baculovirus
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Code CSB-MP822710HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EIF4E3
Uniprot No.
Alternative Names
EIF4E3Eukaryotic translation initiation factor 4E type 3; eIF-4E type 3; eIF-4E3; eIF4E type 3; eIF4E-3
Species
Homo sapiens (Human)
Expression Region
1-224
Target Protein Sequence
MALPPAAAPP AGAREPPGSR AAAAAAAPEP PLGLQQLSAL QPEPGGVPLH SSWTFWLDRS LPGATAAECA SNLKKIYTVQ TVQIFWSVYN NIPPVTSLPL RCSYHLMRGE RRPLWEEESN AKGGVWKMKV PKDSTSTVWK ELLLATIGEQ FTDCAAADDE VIGVSVSVRD REDVVQVWNV NASLVGEATV LEKIYELLPH ITFKAVFYKP HEEHHAFEGG RGKH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis. May act as an inhibitor of EIF4E1 activity.
Gene References into Functions
  1. We performed bioinformatics analyses of ESTs and the 3'UTRs of the main transcript splice variants of the translational initiation factor eIF4E1 and its family members, eIF4E2 and eIF4E3. We propose to elucidate the minor splice variants of eIF4E2 and eIF4E3 in great detail because they might produce proteins with modified features that fulfill different cellular roles from their major counterparts. PMID: 28942592
  2. Following heat shock, eIF4E2 is found in both processing bodies and stress granules, whereas eIF4E3_A relocates only to stress granules. PMID: 27578149
  3. MNK1 and MNK2 inhibition ablates eIF4E1 phosphorylation and concurrently enhances eIF4E3 expression in diffuse large B-cell lymphoma. PMID: 25403230
  4. eIF4E3 impedes oncogenic transformation and its expression is lost in some cancers. PMID: 23587918
Protein Families
Eukaryotic initiation factor 4E family
Database Links

HGNC: 31837

OMIM: 609896

KEGG: hsa:317649

STRING: 9606.ENSP00000393324

UniGene: Hs.581355

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