Product Details

Purity

>85% (SDS-PAGE)

Target Names

DIS3

Uniprot No.

Q9Y2L1

Alternative Names

2810028N01Rik; bA555G22; Dis3; DIS3 homolog; exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog (S. cerevisiae); DIS3 mitotic control homolog; DIS3 mitotic control; S. cerevisiae; homolog of; dis3p; EXOSC11; Exosome complex exonuclease RRP44; exosome component 11; KIAA1008; Mitotic control protein dis3 homolog ; Protein DIS3 homolog; Ribosomal RNA processing protein 44 ; Ribosomal RNA processing protein 44; S. cerevisiae; homolog of; Ribosomal RNA-processing protein 44; RP11-342J4.3; RRP44 ; RRP44_HUMAN

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Gene References into Functions

The 3.8 A resolution cryo-EM structure of the core complex bound to a single-stranded RNA reveals that the RNA channel path is formed by two distinct features of the hDIS3 exoribonuclease: an open conformation and a domain organization more similar to bacterial RNase II than to yeast Rrp44. PMID: 30047866
Results from a study on gene variability markers in early-stage human embryos shows that DIS3 is a putative variability marker for the 3-day, 8-cell embryo stage, identified by mixture models as well as standard ANOVA. PMID: 26288249
Authors have identified a putative functional indel variant at chr13q22.1 that associates with decreased DIS3 expression in carriers of pancreatic cancer risk-increasing alleles, and could therefore affect nuclear RNA processing and/or decay. PMID: 28172817
Data indicate the pathological relevance of exosome component 11 protein (DIS3) mutations in plasma cell dyscrasias and suggest that DIS3 may represent a potential tumor suppressor gene in such disorders. PMID: 26305418
structure and function of DIS3 PMID: 26193331
The study establishes that the ribonuclease DIS3, targeting LIN28B, sustains the maturation of let-7 miRNAs and suggests the increased translation of critical oncogenes as one of the biological outcomes of DIS3 inactivation. PMID: 25925570
seven novel somatic DIS3 single nucleotide variants (SNVs) and defined three hot spot mutations within the RNB domain. PMID: 25521164
Overexpression of DIS3 and LRCH1 associated with adenoma to carcinoma progression is linked to the colorectal cancer specific gain of 13q. PMID: 24478024
Multiple myeloma-associated hDIS3 mutations interfere with hDIS3 exonucleolytic activity. PMID: 24150935
Data show that hDIS3 and hDIS3L are active exonucleases, but only hDIS3 has retained endonucleolytic activity, and suggest that three different ribonucleases can serve as catalytic subunits for the exosome in human cells. PMID: 20531386
S. cerevisiae Dis3p is identical to Rrp44p, which comprises the exosome involved in ribosomal RNA processing, S. cerevisiae Dis3p was found to be localized in the nucleolus. PMID: 9562621
Protein-protein interactions between human exosome components support the assembly of RNase PH-type subunits into a six-membered PNPase-like ring. There appears to be no interaction between human dis3 and other exosome subunits, however. PMID: 12419256
Dis3p is identical to Rrp44p, which comprises the exosome involved in ribosomal RNA processing. Similar to S. cerevisiae Dis3p, human Dis3p enhanced RCC1-stimulated nucleotide release from Ran, in a dose-dependent manner, and bound to GTP- or GDP-Ran. PMID: 9562621

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Subcellular Location

Cytoplasm. Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus.

Protein Families

RNR ribonuclease family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 20604

OMIM: 607533

KEGG: hsa:22894

STRING: 9606.ENSP00000366997

UniGene: Hs.744104

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Recombinant Human Exosome complex exonuclease RRP44 (DIS3), partial

Product Details

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Target Background

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