Recombinant Human F-box/LRR-repeat protein 21 (FBXL21)

Code CSB-YP891953HU
MSDS
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Source Yeast
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Code CSB-EP891953HU
MSDS
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Source E.coli
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Code CSB-EP891953HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891953HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP891953HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBXL21
Uniprot No.
Alternative Names
F box and leucine rich repeat protein 21; F box and leucine rich repeat protein 3 pseudogene; F box and leucine rich repeat protein 3B; F box protein Fbl3b; F box/LRR repeat protein 21; F box/LRR repeat protein 3B; F-box and leucine-rich repeat protein 21; F-box and leucine-rich repeat protein 3B; F-box/LRR-repeat protein 21; F-box/LRR-repeat protein 3B; Fbl 21; FBL 3; FBL 3B; Fbl21; FBL3; FBL3B; FBXL 21; FBXL 3B; FBXL 3P; Fbxl21; FBXL3B; FBXL3P; FXL21_HUMAN; MGC120237
Species
Homo sapiens (Human)
Expression Region
1-434
Target Protein Sequence
MKRNSLSVEN KIVQLSGAAK QPKVGFYSSL NQTHTHTVLL DWGSLPHHVV LQIFQYLPLL DRACASSVCR RWNEVFHISD LWRKFEFELN QSATSSFKST HPDLIQQIIK KHFAHLQYVS FKVDSSAESA EAACDILSQL VNCSIQTLGL ISTAKPSFMN VSESHFVSAL TVVFINSKSL SSIKIEDTPV DDPSLKILVA NNSDTLRLPK MSSCPHVSSD GILCVADRCQ GLRELALNYY ILTDELFLAL SSETHVNLEH LRIDVVSENP GQIKFHAVKK HSWDALIKHS PRVNVVMHFF LYEEEFETFF KEETPVTHLY FGRSVSKVVL GRVGLNCPRL IELVVCANDL QPLDNELICI AEHCTNLTAL GLSKCEVSCS AFIRFVRLCE RRLTQLSVME EVLIPDEDYS LDEIHTEVSK YLGRVWFPDV MPLW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-recognition component of the SCF(FBXL21) E3 ubiquitin ligase complex involved in circadian rhythm function. Plays a key role in the maintenance of both the speed and the robustness of the circadian clock oscillation. The SCF(FBXL21) complex mainly acts in the cytosol and mediates ubiquitination of CRY proteins (CRY1 and CRY2), leading to CRY proteins stabilization. The SCF(FBXL21) complex counteracts the activity of the SCF(FBXL3) complex and protects CRY proteins from degradation. Involved in the hypothalamic suprachiasmatic nucleus (SCN) clock regulating temporal organization of the daily activities.
Subcellular Location
Cytoplasm, cytosol. Nucleus.
Database Links

HGNC: 13600

OMIM: 609087

UniGene: Hs.591275

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