Recombinant Human Fc receptor-like protein 3 (FCRL3), partial

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Code CSB-EP853475HU
MSDS
Size $306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
FCRL3
Uniprot No.
Research Area
Others
Alternative Names
(FcR-like protein 3)(FcRL3)(Fc receptor homolog 3)(FcRH3)(IFGP family protein 3)(hIFGP3)(Immune receptor translocation-associated protein 3)(SH2 domain-containing phosphatase anchor protein 2)(CD antigen CD307c)
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
18-573aa
Target Protein Sequence
GVAPKAVLLLNPPWSTAFKGEKVALICSSISHSLAQGDTYWYHDEKLLKIKHDKIQITEPGNYQCKTRGSSLSDAVHVEFSPDWLILQALHPVFEGDNVILRCQGKDNKNTHQKVYYKDGKQLPNSYNLEKITVNSVSRDNSKYHCTAYRKFYILDIEVTSKPLNIQVQELFLHPVLRASSSTPIEGSPMTLTCETQLSPQRPDVQLQFSLFRDSQTLGLGWSRSPRLQIPAMWTEDSGSYWCEVETVTHSIKKRSLRSQIRVQRVPVSNVNLEIRPTGGQLIEGENMVLICSVAQGSGTVTFSWHKEGRVRSLGRKTQRSLLAELHVLTVKESDAGRYYCAADNVHSPILSTWIRVTVRIPVSHPVLTFRAPRAHTVVGDLLELHCESLRGSPPILYRFYHEDVTLGNSSAPSGGGASFNLSLTAEHSGNYSCDADNGLGAQHSHGVSLRVTVPVSRPVLTLRAPGAQAVVGDLLELHCESLRGSFPILYWFYHEDDTLGNISAHSGGGASFNLSLTTEHSGNYSCEADNGLGAQHSKVVTLNVTGTSRNRTGLT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
67.4 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

The preparation of the recombinant Human FCRL3 protein involves manipulating the expression of the desired gene in e.coli cells. The sequence (18-573aa) of foreign DNA is fused to an expression vector and then introduced into e.coli cells. Selected positive cells are cultured to induce the expression of the desired protein. The protein features a N-terminal 10xHis tag fusion. The recombinant targeted protein is subjected to affinity purification. Its purity exceeds 85% as assessed by SDS-PAGE.

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Target Background

Function
Promotes TLR9-induced B-cell proliferation, activation and survival but inhibits antibody production and suppresses plasma cell differentiation. Enhances activation of NF-kappa-B and MAPK signaling pathways in TLR9 stimulated B-cells. Has inhibitory potentional on B-cell receptor (BCR)-mediated signaling, possibly through association with SH2 domain-containing phosphatases. Inhibits cell tyrosine phosphorylation, calcium mobilization and activation-induced cell death induced through BCR signaling. Regulatory T-cells expressing FCRL3 exhibit a memory phenotype, are relatively nonresponsive to antigenic stimulation in presence of IL2 and have reduced capacity to suppress the proliferation of effector T-cells.
Gene References into Functions
  1. no association between the FCRL-3 rs7528684 SNP with susceptibility to allergic asthma in Iranian North-Western Azeri population. PMID: 28792713
  2. -169CC genotype associated with a beneficial functional effect on residual insulin secretion and HbA1c level dynamics in type 1 diabetes PMID: 27615679
  3. Pooled genome wide association study identified a genetic variant upstream of FCRL3 as a susceptibility locus for Graves' disease in addition to those identified in the Major Histo-compatibility Complex. PMID: 27863461
  4. The expression of Sezary signature genes: FCRL3, Tox, and miR-214, was significantly higher in samples from Sezary syndrome patients with CD164 expressing CD4(+) T cells. PMID: 27766406
  5. This study aims to investigate the association between common polymorphisms of FCRL3 gene and multiple sclerosis risk in a Chinese Han population. PMID: 25862376
  6. Observed no association between the MHC2TA or FCRL3 SNPs and rheumatoid arthritis in Mexican patients. PMID: 26350270
  7. genetic polymorphism is associated with rheumatoid arthritis in Chinese Han population PMID: 26746625
  8. Genetic polymorphisms in FCRL3 are genetic risk factors for neuromyelitis optica in the Chinese population. PMID: 25575677
  9. FCRL3_3, FCRL3_5 and FCRL3_6 polymorphisms could increase susceptibility to Graves' disease only in Asians, rather than in Caucasians. PMID: 26321232
  10. CTLA4 and FCRL3 genes overexpression may play an important role in children suffering from autoimmune thyroiditis. PMID: 26994388
  11. FCRL3 genetic polymorphisms were associated with an increased risk of endometriosis-related infertility, regardless of symptoms. PMID: 26334889
  12. 4 Single Nucleotide Polymorphisms (rs7528684, rs945635, rs3761959, and rs2282284) could significantly elevate the risk of Neuromyelitis Optica in Chinese Han population. PMID: 26402798
  13. study found a significant association between the SNPs in FCRL3 gene and allergic rhinitis (AR) in Chinese Han patients; results suggest these gene polymorphisms might be the autoimmunity risk for AR PMID: 25594855
  14. Association between FCRL3 polymorphisms and increased risk of sudden sensorineural hearing loss in a Chinese Han population. PMID: 26051414
  15. the TIGIT/FCRL3 combination allows reliable identification of Helios(+) Treg cells even in highly activated conditions in vitro as well as in PBMCs of autoimmune patients. PMID: 25762785
  16. CONCLUSIONS: Our study demonstrated that the FCRL3 -169T>C polymorphism is not a risk factor of systemic lupus erythematosus in the Polish population, but this polymorphism may contribute to autoantibody production in this disease. PMID: 24593204
  17. the FCRL3 polymorphisms are associated with not only autoimmune diseases including RA, GD, T1D, and other disease under different genetic models, but also different ethnic subgroups. PMID: 24117236
  18. meta-analysis of published studies including 2,544 patients and 3,913 controls demonstrates that the FCRL3 -169 C/T polymorphism does not confer susceptibility to systemic lupus erythematosus in Europeans or Asians PMID: 23512175
  19. FCRL3 -169T>C polymorphism alters the expression of FCRL3 and can be a risk factor of endometriosis-related infertility. PMID: 23553198
  20. This meta-analysis demonstrates that the FCRL3 -169 C/T polymorphism may confer susceptibility to seropositive RA in Asians.[meta-analysis] PMID: 23777926
  21. The results of this study showed a significant association between FCRL3-110 A/G polymorphism and susceptibility to rheumatoid arthritis. PMID: 23883198
  22. FCRL3 amplified the NF-kappaB and mitogen-activated protein kinase signaling cascades, and halted CpG triggered BLIMP1 induction in an ERK-dependent fashion. PMID: 23857366
  23. FCRL3 gene polymorphism shows positive relatationship in the development of rheumatoid arthritis. PMID: 23463945
  24. FCRL3 gene and its proxy SNP rs7528684 may be involved in the pathogenesis of GD by excessive inhibiting B cell receptor signaling and the impairment of suppressing function of Tregs. PMID: 23505439
  25. CD40 and FCRL3 gene polymorphisms were associated with autoimmune hyperthyroidism intractability. PMID: 22706687
  26. A male-gender juvenile rheumatoid arthritis and asthma, but not childhood-onset systemic lupus erythematosus are associated with FCRL3 polymorphisms in Mexicans. PMID: 23070121
  27. Polymorphisms of the FCRL3 gene may contribute to the progression of joint destruction rather than susceptibility to rheumatoid arthritis. PMID: 22386693
  28. A functional variant in FCRL3 is associated with higher Fc receptor-like 3 expression on T cell subsets and rheumatoid arthritis disease activity. PMID: 22392608
  29. Findings support a possible gene-gene interaction for FOXP3/FCRL3 polymorphisms, leading to a cumulative effect on endometriosis development. PMID: 22341374
  30. Frequencies of specific FcRL3 haplotypes are significantly associated with a Chinese cohort of Guillain-Barre syndrome patients compared with healthy controls. PMID: 22458979
  31. FCRL3 -169C/C genotype is associated with anti-citrullinated protein antibody-positive rheumatoid arthritis and with radiographic progression. PMID: 21885492
  32. genetic polymorphism is associated with endometriosis in Brazilian population PMID: 21663782
  33. the FCRL3 C-169T polymorphism may play an important role in the pathogenesis of endometriosis and/or infertility. PMID: 21529967
  34. The functional FCRL3 SNP -169T/C appears to play important roles in the development of certain phenotypes such as systemic lupus erythematosus leukopenia and rheumatoid arthritis disease severity in Taiwanese patients with these diseases. PMID: 21078711
  35. A significant association of fcrl3_3 with primary biliary cirrhosis only in Japanese. PMID: 21299530
  36. These results suggest that FCRL3 polymorphisms and haplotypes may contribute to genetic susceptibility to rheumatoid arthritis in a Chinese population. PMID: 20732364
  37. PTPN22 rs3789604 and FCRL3 rs7528684 polymorphisms are protective against Graves' disease. PMID: 19438904
  38. Meta-analysis suggests that the FCRL3 -169 C/T polymorphism is a significant risk factor for rheumatoid arthritis in Asians, but not in Europeans. PMID: 19690864
  39. FcRL3 expression is associated with T(reg) dysfunction PMID: 20190142
  40. The polymorphisms of the promoter A/G,exon 2 C/G,exon 4 C/T in the FcRL3 gene were risk factors to Graves disease in Chongqing Han population. PMID: 19953494
  41. The FCRL3 gene does not appear associated with susceptibility to HLA-B27-positive ankylosing spondylitis in Han Chinese population. PMID: 19657722
  42. This meta-analysis demonstrates that the FCRL3 169CC genotype (recessive effect) may confer susceptibility to systemic lupus erythematosus, especially in Asian-derived population. PMID: 19565352
  43. molecular cloning and characterization of SPAP2 PMID: 12051764
  44. Single nucleotide polymorphism in Graves' disease in a large UK Caucasian Graves' disease data set. PMID: 16384851
  45. This gene will not have a substantial effect in determining susceptibility to RArheumatoid arthritis in populations of Northern European descent. PMID: 16859508
  46. Susceptibility to type 1 autoimmune hepatitis in Japanese patients is not influenced by FcgammaRIIA, FcgammaRIIB, or FCRL3 polymorphisms. PMID: 17020818
  47. Findings from a large case-control sample of patients with alopecia areata (AA) do not support an association between FCRL3 and susceptibility to AA. PMID: 17117947
  48. Both the FCRL3 and PTPN22 genes play roles in rheumatoid arthritis susceptibility, but in different individuals. PMID: 17133579
  49. The association of the -169C/T SNP in FCRL3 with rheumatoid arthritis and systemic lupus erythematosus that was observed in Japanese patients was not replicated in a Korean population. PMID: 17133581
  50. Susceptibility to autoimmunity at the FCRL3 locus. PMID: 17200162

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Involvement in disease
Rheumatoid arthritis (RA)
Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Tissue Specificity
Primarily expressed in secondary lymphoid tissues by mature subsets of B-cells. Low expression on transitional B cells which increases to higher surface expression on mature and memory B-cells with innate-like features (at protein level). Expressed a low
Database Links

HGNC: 18506

OMIM: 180300

KEGG: hsa:115352

STRING: 9606.ENSP00000357167

UniGene: Hs.292449

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