Name: Recombinant Human Fibrinogen beta chain (FGB), partial
Brand: CUSABIO
SKU: CSB-EP008608HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

FGB

Uniprot No.

P02675

Alternative Names

Beta fibrinogen; Epididymis Secretory Sperm Binding Protein Li 78p; FGB; FIBB_HUMAN; Fibrinogen beta chain; Fibrinogen; B Beta Polypeptide; HEL-S-78p

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

45-491aa

Target Protein Sequence

GHRPLDKKREEAPSLRPAPPPISGGGYRARPAKAAATQKKVERKAPDAGGCLHADPDLGVLCPTGCQLQEALLQQERPIRNSVDELNNNVEAVSQTSSSSFQYMYLLKDLWQKRQKQVKDNENVVNEYSSELEKHQLYIDETVNSNIPTNLRVLRSILENLRSKIQKLESDVSAQMEYCRTPCTVSCNIPVVSGKECEEIIRKGGETSEMYLIQPDSSVKPYRVYCDMNTENGGWTVIQNRQDGSVDFGRKWDPYKQGFGNVATNTDGKNYCGLPGEYWLGNDKISQLTRMGPTELLIEMEDWKGDKVKAHYGGFTVQNEANKYQISVNKYRGTAGNALMDGASQLMGENRTMTIHNGMFFSTYDRDNDGWLTSDPRKQCSKEDGGGWWYNRCHAANPNGRYYWGGQYTWDMAKHGTDDGVVWMNWKGSWYSMRKMSMKIRPFFPQQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

54.8kDa

Protein Length

partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CUSABIO transfected the expression vector which inserted the recombinant DNA into the E.coli, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 6xHis tag sequence was appended to the gene coding for the E.coli of the human FGB protein to form the recombinant DNA. The recombinant human FGB was expressed as N-terminal 6xHis-tagged fusion. The purity of the protein is greater than 90% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 60 kDa.

Fibrinogen is a large, complex, fibrous glycoprotein with three pairs of polypeptide chains linked together by 29 disulfide bonds. Fibrinogen is the major plasma protein coagulation factor. Low plasma fibrinogen concentrations are therefore associated with an increased risk of bleeding due to impaired primary and secondary haemostasis. Fibrinogen is a classical positive acute-phase reactant protein and is an independent predictor of coronary heart disease events. Plasma fibrinogen is an important component of the coagulation cascade, as well as a major determinant of blood viscosity and blood flow. Increasing evidence from epidemiological studies suggests that elevated plasma fibrinogen levels are associated with an increased risk of cardiovascular disorders, including ischaemic heart disease (IHD), stroke and other thromboembolism. This increase in plasma fibrinogen levels may promote a prothrombotic or hypercoagulable state, and may in part explain the risk of stroke and thromboembolism in conditions such as atrial fibrillation.

Target Background

Function

Cleaved by the protease thrombin to yield monomers which, together with fibrinogen alpha (FGA) and fibrinogen gamma (FGG), polymerize to form an insoluble fibrin matrix. Fibrin has a major function in hemostasis as one of the primary components of blood clots. In addition, functions during the early stages of wound repair to stabilize the lesion and guide cell migration during re-epithelialization. Was originally thought to be essential for platelet aggregation, based on in vitro studies using anticoagulated blood. However subsequent studies have shown that it is not absolutely required for thrombus formation in vivo. Enhances expression of SELP in activated platelets. Maternal fibrinogen is essential for successful pregnancy. Fibrin deposition is also associated with infection, where it protects against IFNG-mediated hemorrhage. May also facilitate the antibacterial immune response via both innate and T-cell mediated pathways.

Gene References into Functions

High fibrinogen expression is associated with esophageal carcinoma. PMID: 29134563
Compared with the patients with TT genotypes of fibrinogen beta, expressions of fibrinogen, IL-6 and CRP were significantly higher in the patients with the CC and CT genotypes. PMID: 29165755
Two novel fibrinogen Bbeta chain mutations in two Slovak families with hypofibrinogenemia/afibrinogenemia have been described. PMID: 29286337
FGB c.490G>A indicated the activation of a cryptic splice site causing the insertion of 99 bp in intron 3. This splicing abnormality led to the production of a Bbeta-chain possessing 33 aberrant amino acids, including two Cys residues in the coiled-coil domain. Therefore, a splicing abnormality may cause impaired fibrinogen assembly and secretion. PMID: 29156616
FGB mutations leading to congenital hypofibrinogenaemia PMID: 28306188
Fibrinogen Bbeta448Lys variant is associated with thrombotic fibrin clots in type 2 diabetic patients independently of traditional risk factors. PMID: 27929198
There was underexpression of the majority of genes after sunitinib treatment. The lower expression levels of IGFBP1, CCL20, CXCL6 and FGB were confirmed by qRT-PCR in all cases. The downregulation of gene expression leads us to search for methylation as a mechanism of action of the tyrosine kinase inhibitors PMID: 27834463
elevated levels of plasma homocysteine /homocysteine thiolactone contribute to AD pathology via the Abeta-fibrin(ogen) interaction PMID: 27090576
procoagulant changes in fibrin metastructure appear to result from excessive carbonylation of fibrinogen, which may compensate for a decrease in fibrinogen level in patients with cirrhosis PMID: 26833718
Prognostic impact of plasma fibrinogen levels in patients with esophageal squamous cell carcinoma PMID: 28064398
a tight cluster of alternating multiple splicing regulatory elements and U1 snRNA binding sites controls cryptic splice donor usage throughout the human fibrinogen Bbeta-chain gene (FGB) exon 7 PMID: 28039323
The plasma fibrinogen level was found to be a possible biomarker for clinical response to chemotherapy and postoperative metastasis or death in advanced breast cancer patients who received neoadjuvant chemotherapy PMID: 28621233
Data suggest that, in the binding of fibrin beta N-domains and the (1-8) peptide fragment of VLDLR (very low density lipoprotein receptor), the second and third Lys/Arg clusters in fibrin make major contributions to this interaction while the contribution of the first cluster is moderate. PMID: 28437098
The novel missense mutation in the FGB gene causes afibrinogenemia most probably by affecting the secretion of the fibrinogen beta-chain. PMID: 27824214
fibrinogen aggregation is accompanied by the formation of beta-sheet conformation, and induction of non-native helical segments in the protein inhibits aggregation PMID: 27150313
fibrinogen is an independent predictor of the angiographic presence of coronary artery disease in hypertensive patients. PMID: 27553289
novel mutation was identified in exon 2 of FGB caused by c.221G> T (dagger) substitution PMID: 27812779
Our results show that higher levels of circulating chemerin, CRP, fibrinogen, and ESR are associated with an increased risk of developing colorectal cancer PMID: 26628300
We assessed the diagnostic accuracy of a newly developed laboratory score-based on CA125, platelet count (PLT), C-reactive protein (CRP), and fibrinogen levels-in the preoperative diagnosis of adnexal mass PMID: 26499778
the mechanical properties of individual fibrin fibers formed from blood plasma, were examined. PMID: 27028649
Here we report a new B[beta] gene mutation (Fibrinogen St Kilda) identified in two Caucasian sisters with reduced fibrinogen level (1.2-1.6 g/L) during investigation for recurrent early miscarriages. PMID: 26308135
desAB fibrin binds to prothrombin through the Bbeta26-42 amino acid residues and the formation of such a complex causes a non-enzymatic activation of prothrombin PMID: 26317125
Strong positive association has been found between betatrophin, plasma fibrinogen (FBG), and insulin resistance in non-diabetic subjects. Correlations with FBG and insulin resistance were diminished in type 2 diabetes subjects. PMID: 26077345
These experiments demonstrate, for the FGB-p.Asp185Asn mutation, a pathogenic mechanism not common for fibrinogen deficiencies, i.e. the hyperglycosylation of the Bbeta chain due to the introduction of a novel N-glycosylation motif. PMID: 26006300
High plasma fibrinogen levels and platelet count are associated with esophageal squamous cell carcinoma. PMID: 25896470
Results suggest that FGB -148 C/T and -854 G/A polymorphisms probably contribute to susceptibility of ischemic stroke in Chinese population. PMID: 25890854
The results of the meta-analysis indicate that the -455 G>A polymorphism in the beta-fibrinogen gene is a susceptibility marker of ischemic cerebral infarction in the Chinese population. PMID: 25398500
In the present study, we found that the -148C>T polymorphism in the FGB gene was significantly associated with ischemic stroke in a Chinese population [meta-analysis] PMID: 25867317
This prospective study in 110 patients undergoing major cardiovascular surgery at risk of post-cardiopulmonary bypass bleeding compares fibrinogen level. PMID: 26011420
the NGR motif in fibrinogen is the site that is primarily responsible for the interaction with resting alphaIIbbeta3 and is responsible for triggering platelet activation PMID: 25413489
Elevated fibrinogen levels are associated with negative tumor response to therapy in rectal cancer. PMID: 25384698
Four novel FGB mutations were identified in two afibrinogenemic (one new-born and one 30 years old male) and hypofibrinogenemic (a 49 years old female) patient, with heterogeneous thrombotic and bleeding phenotype PMID: 24560896
plasma fibrinogen increase during ischemic stroke has a role in worse outcome PMID: 24531853
meta-analysis suggests that the FGB-455G/A polymorphism contributes to susceptibility to ischemic stroke and coronary heart disease PMID: 24448059
results of our meta-analysis suggested that the-148C>T polymorphism in the FGB gene is a susceptibility marker of ischemic stroke PMID: 24720800
The A + genotype of the FGB -455 G/A polymorphism associated with poor survival among 55-71 years old Caucasian women in the Finnish stroke cohort. PMID: 24957141
results indicate that FGbeta-455G/A polymorphism may be a susceptible predictor of ischemic stroke [meta-analysis] PMID: 24366241
Elevated fibrinogen is associated with idiopathic sudden hearing loss. PMID: 24466284
High fibrinogen levels are associated with gynecologic cancer. PMID: 25204086
Both fibrinogen polymorphisms are capable to modify the atherosclerotic process via their effects on the coagulation cascade. PMID: 23931975
The beta-fibrinogen -455G/A gene polymorphism is not a risk factor for ischaemic stroke in a Polish population. PMID: 23650004
A meta-analysis of a Chinese population found that the FgB -455G/A and the -148C/T gene polymorphism was implied to be associated with coronary artery disease susceptibility. PMID: 23129316
Both isoforms of the beta-chain of FIB discovered by 2D-gel electrophoresis are decreased in the Parkinson's disease group cerebrospinal fluid, compared to normal controls. PMID: 22889670
Lys, as an inhibitor of protein glycation, improved fibrinogen's structure and function, both in vitro and in vivo. PMID: 22575419
Fibrinogen polymorphisms contributes to the association between common variants in the fibrinogen gene and the risk of developing sporadic cerebral hemorrhage. PMID: 22386478
study found that -C148T FGB polymorphism was an independent predictor of pre- and early postoperative C-reactive protein levels in coronary artery bypass graft patients PMID: 21499712
genetic polymorphism is associated with plasma fibrinogen levels and haematological traits in whites, blacks and mexican americans PMID: 22273812
Levels of fibrinogen and thromboelastometry fibrin polymerisation following treatment with desmopressin (DDAVP). PMID: 22293628
The BF -455G/A polymorphisms was found to be positively. PMID: 21241403
Fibrinogen has chaperone activity which is compromised upon glycation by methylglyoxal. PMID: 22053176

Hide All

Involvement in disease

Congenital afibrinogenemia (CAFBN); Dysfibrinogenemia, congenital (DYSFIBRIN)

Subcellular Location

Secreted.

Tissue Specificity

Detected in blood plasma (at protein level).

Database Links

HGNC: 3662

OMIM: 134830

KEGG: hsa:2244

STRING: 9606.ENSP00000306099

UniGene: Hs.300774

Code	CSB-EP008608HU
Abbreviation	Recombinant Human FGB protein, partial
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP008608HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) FGB. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP008608HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) FGB.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Fibrinogen beta chain (FGB), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage