Recombinant Human Forkhead box protein H1 (FOXH1)

Code CSB-YP008817HU
MSDS
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Source Yeast
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Code CSB-EP008817HU
MSDS
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Source E.coli
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Code CSB-EP008817HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008817HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP008817HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FOXH1
Uniprot No.
Alternative Names
FAST 1; Fast 2; Fast-1; Fast-2; FAST1; Fast2; Forkhead activin signal transducer 1; Forkhead activin signal transducer 2; Forkhead box H1; Forkhead box protein H1; FOX H1; FOXH 1; FOXH1; FOXH1_HUMAN; hFAST 1; hFAST-1; hFAST1; Human homolog of Xenopus forkhead activin signal transducer 1; TGF beta/Activin signal transducer FAST1
Species
Homo sapiens (Human)
Expression Region
1-365
Target Protein Sequence
MGPCSGSRLG PPEAESPSQP PKRRKKRYLR HDKPPYTYLA MIALVIQAAP SRRLKLAQII RQVQAVFPFF REDYEGWKDS IRHNLSSNRC FRKVPKDPAK PQAKGNFWAV DVSLIPAEAL RLQNTALCRR WQNGGARGAF AKDLGPYVLH GRPYRPPSPP PPPSEGFSIK SLLGGSGEGA PWPGLAPQSS PVPAGTGNSG EEAVPTPPLP SSERPLWPLC PLPGPTRVEG ETVQGGAIGP STLSPEPRAW PLHLLQGTAV PGGRSSGGHR ASLWGQLPTS YLPIYTPNVV MPLAPPPTSC PQCPSTSPAY WGVAPETRGP PGLLCDLDAL FQGVPPNKSI YDVWVSHPRD LAAPGPGWLL SWCSL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator. Recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. Required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. Forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element).
Gene References into Functions
  1. These results indicate that Smad4 acts as a tumor suppressor by activating FOXH1, and then suppressing the expression of estrogen receptor, in addition to tumor migration and invasion. PMID: 25482028
  2. HEB and E2A-bind the SCA motif at regions overlapping SMAD2/3 and FOXH1 PMID: 21828274
  3. Four genetic variants are found in FOXH1 that are associated with ventricular septal defects in Chinese patients. PMID: 19525021
  4. Results suggest that PKA can negatively regulate ERalpha, at least in part, through FoxH1. PMID: 19711044
  5. This work suggests that FAST-1 may participate in the vascular smooth muscle response to injury and may represent a potential molecular target for modulating the progression of cardiac allograft vasculopathy. PMID: 15737749
  6. Smad-binding peptide aptamer FOXH1 can be developed to selectively inhibit TGF-beta-induced gene expression. PMID: 15750622
  7. These results demonstrate for a functional role for TGF-beta ligands in regulation of mammalian Mixl1, identify FoxH1 as an essential co-activator, and implicate Nodal as the embryonic regulator of Mixl1 in mesendoderm morphogenesis. PMID: 15982639
  8. FoxH1 has a role in androgen receptor-mediated transactivation PMID: 16120611
  9. Reduced NODAL signaling via mutation of several pathway members including FOXH1 is linked to heart defects and holoprosencephaly. PMID: 18538293

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Subcellular Location
Nucleus.
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 3814

OMIM: 603621

KEGG: hsa:8928

STRING: 9606.ENSP00000366534

UniGene: Hs.708365

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