Recombinant Human G protein-coupled receptor kinase 6 (GRK6)

Code CSB-YP009927HU
MSDS
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Source Yeast
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Code CSB-EP009927HU
MSDS
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Source E.coli
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Code CSB-EP009927HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009927HU
MSDS
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Source Baculovirus
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Code CSB-MP009927HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRK6
Uniprot No.
Alternative Names
FLJ32135; G protein coupled receptor kinase 6; G protein coupled receptor kinase GRK6; G protein-coupled receptor kinase 6; G protein-coupled receptor kinase GRK6; Gprk6; Grk6; GRK6_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-576
Target Protein Sequence
MELENIVANT VLLKAREGGG GNRKGKSKKW RQMLQFPHIS QCEELRLSLE RDYHSLCERQ PIGRLLFREF CATRPELSRC VAFLDGVAEY EVTPDDKRKA CGRQLTQNFL SHTGPDLIPE VPRQLVTNCT QRLEQGPCKD LFQELTRLTH EYLSVAPFAD YLDSIYFNRF LQWKWLERQP VTKNTFRQYR VLGKGGFGEV CACQVRATGK MYACKKLEKK RIKKRKGEAM ALNEKQILEK VNSRFVVSLA YAYETKDALC LVLTLMNGGD LKFHIYHMGQ AGFPEARAVF YAAEICCGLE DLHRERIVYR DLKPENILLD DHGHIRISDL GLAVHVPEGQ TIKGRVGTVG YMAPEVVKNE RYTFSPDWWA LGCLLYEMIA GQSPFQQRKK KIKREEVERL VKEVPEEYSE RFSPQARSLC SQLLCKDPAE RLGCRGGSAR EVKEHPLFKK LNFKRLGAGM LEPPFKPDPQ AIYCKDVLDI EQFSTVKGVE LEPTDQDFYQ KFATGSVPIP WQNEMVETEC FQELNVFGLD GSVPPDLDWK GQPPAPPKKG LLQRLFSRQD CCGNCSDSEE ELPTRL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis. Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor: LRP6 during Wnt signaling (in vitro).
Gene References into Functions
  1. GRK6 overexpression can significantly enhance tumor proliferation and invasion, which was consistent with clinical findings. PMID: 29805156
  2. Genetic variation in OXTR and GRK6 is associated with the amount of oxytocin required as well as the duration of labor and risk for cesarean delivery among women undergoing induction of labor near term. PMID: 28526450
  3. This work demonstrates the importance of GRK6 in regulation of hematopoietic stem cell self-renewal and reveals its potential role in participation of stress response. PMID: 27882944
  4. Decreased expression of GRK6 may serve as an independent predictor of overall survival in lung adenocarcinoma. PMID: 27601164
  5. The expression of GRK6 mRNA and protein was significantly lower in hypopharyngeal squamous cell carcinoma than in corresponding adjacent non-tumor tissues. This was associated with aberrant methylation of the gene. PMID: 26718636
  6. Our results imply that GRK6 may not play a role in the pathophysiology of schizophrenia among Han Chinese PMID: 24302161
  7. these results indicate that GRK6 complexes with AGS3-Galphai2 to regulate CXCR2-mediated leukocyte functions at different levels, including downstream effector activation, receptor trafficking, and expression at the cell membrane. PMID: 24510965
  8. GRK6 plays a predominant role in phosphorylation of FFA receptor (FFA)4. PMID: 24412271
  9. results suggested that GRK6 overexpression plays an important role in hepatocellular carcinoma PMID: 23375037
  10. Downregulation of GRK6 expression enhances CXCR4 signaling and promoted medulloblastoma cell migration. PMID: 23497290
  11. A reduced cortical concentration of GRK6 in schizophrenia (resembling that in aging) may result in altered G protein-dependent signaling, thus contributing to prefrontal deficits in schizophrenia. PMID: 21784156
  12. inhibition of GRK6 represents a uniquely targeted novel therapeutic strategy in human multiple myeloma. PMID: 19996089
  13. A number of double mutations of GRK6 kinase within helices 3, 9, and 10 reduced phosphorylation of the beta2 adrenergic receptor and rhodopsin compared to wild-type GRK6 kinase. PMID: 20038610
  14. Recruitment of beta-Arrestin 2, but not beta-Arrestin 1, to the active WHIM-mutant receptor is delayed compared to the WT CXCR4 receptor and Grk6 fails to associate with the WHIM-mutant receptor. PMID: 19956569
  15. potential role of endogenous GRK6 in the regulation of M(3) mACh receptor PMID: 11856737
  16. GRK6 is potential marker for organ injury and survival after cardiopulmonary bypass PMID: 12552191
  17. the CRH-R1alpha carboxyl tail is important for regulation of receptor activity by G protein-coupled receptor kinase PMID: 15498832
  18. the crystalline GRK6 RGS homology domain forms an extensive dimer interface using conserved hydrophobic residues distinct from those in GRK2 that bind Galpha(q) PMID: 16613860
  19. These results suggest that the C-terminus of GRK6A contains a novel electrostatic palmitoyl switch in which acidic residues weaken the membrane-binding strength of the amphipathic helix.[GRK6A] PMID: 17538017
  20. oxytocin receptor responsiveness is regulated by G protein-coupled receptor kinase 6 in human myometrial smooth muscle PMID: 19423652

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Subcellular Location
Membrane; Lipid-anchor.
Protein Families
Protein kinase superfamily, AGC Ser/Thr protein kinase family, GPRK subfamily
Tissue Specificity
Widely expressed.
Database Links

HGNC: 4545

OMIM: 600869

KEGG: hsa:2870

STRING: 9606.ENSP00000433511

UniGene: Hs.235116

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