Recombinant Human G0/G1 switch protein 2 (G0S2)

Code CSB-YP009114HU
MSDS
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Source Yeast
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Code CSB-EP009114HU
MSDS
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Source E.coli
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Code CSB-EP009114HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009114HU
MSDS
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Source Baculovirus
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Code CSB-MP009114HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
G0S2
Uniprot No.
Alternative Names
G0/G1 switch regulatory protein 2; G0/G1switch 2; G0s2; G0S2_HUMAN; OTTHUMP00000034644; Putative lymphocyte G0/G1 switch protein 2 ; RP1 28O10.2
Species
Homo sapiens (Human)
Expression Region
1-103
Target Protein Sequence
METVQELIPL AKEMMAQKRK GKMVKLYVLG SVLALFGVVL GLMETVCSPF TAARRLRDQE AAVAELQAAL ERQALQKQAL QEKGKQQDTV LGGRALSNRQ HAS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Promotes apoptosis by binding to BCL2, hence preventing the formation of protective BCL2-BAX heterodimers.
Gene References into Functions
  1. ER+ breast cancer cells with restored G0S2 expression had a relative increased sensitivity to tamoxifen PMID: 28910567
  2. Palmitate can induce lipid accumulation in HepG2 cells by activating C/EBPbeta-mediated G0S2 expression. PMID: 29209111
  3. G0S2 functions as a master regulator of tissue-specific balance of TG storage vs. mobilization, partitioning of metabolic fuels between adipose and liver, and the whole-body adaptive energy response. PMID: 28645852
  4. PML/RARalpha synergizes with C/EBPepsilon to reactivate the C/EBPepsilon target G0S2, thereby contributing to All-trans retinoic acid -mediated acute promyelocytic leukemia differentiation and potentially, clinical remission. PMID: 27605212
  5. differences in G0S2 expression may explain depot-specific and obesity-associated differences in lipolysis on the molecular level PMID: 26707160
  6. Data indicate that a tumor suppressor mechanism by which G0/G1 switch gene 2 product (G0S2) directly inhibits activity of a key intracellular adipose triglyceride lipase (ATGL). PMID: 26318046
  7. Results indicate that G0S2 acts as a prosurvival molecule in endothelial cells. PMID: 25588877
  8. Data indicate that the peptide corresponding to residues Lys-20 to Ala-52 from G0S2 Inhibits ATGL in the nanomolar range. PMID: 25258314
  9. reelin expression is altered by Abeta leading to impaired reelin signaling. PMID: 23951308
  10. A new mechanism that controls proliferation in K562 cells, suggesting a possible tumor suppressor function for G0S2 in leukemia cells. PMID: 24183236
  11. This linked G0S2 subcellular localization to G0S2 transcriptional repression. The potential mechanisms responsible for this G0S2 repression are examined. PMID: 23546556
  12. Data indicate that downregulation of G0S2 in adipose tissue could represent one of the underlying causes leading to increased lipolysis in the insulin-resistant state. PMID: 22891293
  13. Gene expression profiles showed that G0/G1 switch 2 was up-regulated in epidermolysis bullosa subtypes. PMID: 22716248
  14. Reduced mRNA and protein content of Plin and G0S2 and borderline increased ATGL protein in sc adipose tissue from poorly controlled type 2 diabetic subjects. PMID: 22535977
  15. findings are compatible with the notion that the ATGL-G0S2 complex is an important long-term regulator of lipolysis under physiological conditions such as fasting in humans PMID: 21613358
  16. DNA methylation of G0S2 can be an important biomarker for squamous lung cancer. PMID: 19816938
  17. mRNA expression of G0S2 was regulated mainly by DNA methylation in squamous lung cancer cell lines. PMID: 19878646
  18. A novel putative target gene of PPARalpha, G0S2 (G0/G1 switch gene 2) was identified and characterized. PMID: 16086669
  19. G0S2 is an all-trans-retinoic acid target gene PMID: 18636162
  20. G0S2 encodes a mitochondrial protein that specifically interacts with Bcl-2 and promotes apoptosis by preventing the formation of protective Bcl-2/Bax heterodimers PMID: 19706769

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Subcellular Location
Mitochondrion.
Tissue Specificity
Widely expressed with highest levels in peripheral blood, skeletal muscle and heart, followed by kidney and liver.
Database Links

HGNC: 30229

OMIM: 614447

KEGG: hsa:50486

STRING: 9606.ENSP00000355996

UniGene: Hs.432132

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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