Recombinant Human G2 and S phase-expressed protein 1 (GTSE1)

Code CSB-YP889155HU
MSDS
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Source Yeast
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Code CSB-EP889155HU
MSDS
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Source E.coli
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Code CSB-EP889155HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP889155HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP889155HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GTSE1
Uniprot No.
Alternative Names
GTSE1; G2 and S phase-expressed protein 1; GTSE-1; Protein B99 homolog
Species
Homo sapiens (Human)
Expression Region
1-720
Target Protein Sequence
MDDPKKEDIL LLADEKFDFD LSLSSSSANE DDEVFFGPFG HKERCIAASL ELNNPVPEQP PLPTSESPFA WSPLAGEKFV EVYKEAHLLA LHIESSSRNQ AAQAAKPEDP RSQGVERFIQ ESKLKINLFE KEKEMKKSPT SLKRETYYLS DSPLLGPPVG EPRLLASSPA LPSSGAQARL TRAPGPPHSA HALPRESCTA HAASQAATQR KPGTKLLLPR AASVRGRSIP GAAEKPKKEI PASPSRTKIP AEKESHRDVL PDKPAPGAVN VPAAGSHLGQ GKRAIPVPNK LGLKKTLLKA PGSTSNLARK SSSGPVWSGA SSACTSPAVG KAKSSEFASI PANSSRPLSN ISKSGRMGPA MLRPALPAGP VGASSWQAKR VDVSELAAEQ LTAPPSASPT QPQTPEGGGQ WLNSSCAWSE SSQLNKTRSI RRRDSCLNSK TKVMPTPTNQ FKIPKFSIGD SPDSSTPKLS RAQRPQSCTS VGRVTVHSTP VRRSSGPAPQ SLLSAWRVSA LPTPASRRCS GLPPMTPKTM PRAVGSPLCV PARRRSSEPR KNSAMRTEPT RESNRKTDSR LVDVSPDRGS PPSRVPQALN FSPEESDSTF SKSTATEVAR EEAKPGGDAA PSEALLVDIK LEPLAVTPDA ASQPLIDLPL IDFCDTPEAH VAVGSESRPL IDLMTNTPDM NKNVAKPSPV VGQLIDLSSP LIQLSPEADK ENVDSPLLKF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in p53-induced cell cycle arrest in G2/M phase by interfering with microtubule rearrangements that are required to enter mitosis. Overexpression delays G2/M phase progression.
Gene References into Functions
  1. G2 and S-phase expressed 1 (GTSE1) expression promotes AM progression and correlates with clinical outcomes of patients with acral melanoma (AM), and may represent a promising therapeutic target to suppress AM progression. PMID: 29660787
  2. GTSE1 is expressed exclusively in late G2 and M phase. From nuclear envelope breakdown until anaphase onset, GTSE1 binds preferentially to the most stable mitotic spindle microtubules and promotes their turnover. PMID: 28821562
  3. Results indicated for the first time that overexpression of GTSE1 was involved in the progress of HCC, enhancing proliferation and promoting cell invasion in HCC cells. PMID: 27240802
  4. Study identifies GTSE1 as a biomarker for cisplatin resistance in gastric cancer cells and suggests its repressive role in cisplatin induced apoptosis making it a potential therapeutic target for better clinical management of gastric cancer patients. PMID: 26209226
  5. GTSE1 is a microtubule plus-end tracking protein that regulates EB1-dependent cell migration. PMID: 23236459
  6. GTSE1 is overexpressed dramatically in lung cancer patients' tissues. PMID: 22292647
  7. Data show that G2 and S-phase-expressed 1 (GTSE1) protein, a negative regulator of p53, is required for G2 checkpoint recovery and that Plk1 phosphorylation of GTSE1 promotes its nuclear localization. PMID: 20577264
  8. hGTSE-1 mediated-p21(CIP1/WAF1) stabilization is clearly involved in the ability of cells to counteract cytotoxicity induced by the microtubule poison paclitaxel. PMID: 20018861
  9. GTSE-1 controls DNA damage-induced apoptosis by affecting p53 function PMID: 12750368
  10. requires an intact nuclear export signal and functional Mdm2 for the regulation of p53 PMID: 14707141

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Subcellular Location
Cytoplasm, cytoskeleton. Note=Associated with microtubules.
Database Links

HGNC: 13698

OMIM: 607477

KEGG: hsa:51512

STRING: 9606.ENSP00000415430

UniGene: Hs.386189

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