Recombinant Human General vesicular transport factor p115 (USO1), partial

Code CSB-YP025694HU
MSDS
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Source Yeast
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Code CSB-EP025694HU
MSDS
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Source E.coli
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Code CSB-EP025694HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025694HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP025694HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
USO1
Uniprot No.
Alternative Names
General vesicular transport factor; General vesicular transport factor p115; P115; Protein USO1 homolog; TAP; Transcytosis associated protein; Transcytosis-associated protein; Uso1; USO1_HUMAN; VDP; Vesicle docking protein; Vesicle docking protein p115; Vesicle-docking protein
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.
Gene References into Functions
  1. USO1 gene may be a promising target for the therapy of human multiple myeloma and its diagnosis marker. PMID: 26898451
  2. USO1 gene has a critical role in human colon cancer: USO1 is involved in cell proliferation and cell migration of colon cancer cells. PMID: 26184508
  3. P115 promotes proliferation of gastric cancer cells through an interaction with MIF. PMID: 24379579
  4. Scyl1 interacts with 58K/formiminotransferase cyclodeaminase (FTCD) and golgin p115, and is required for the maintenance of Golgi morphology. PMID: 20209057
  5. p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis PMID: 21536679
  6. a conserved tethering function of the Golgi protein p115 CTF which promotes p53-ERK interaction for the amplification of the apoptotic signal. PMID: 21147777
  7. Data show that the number of tandem ARM repeats in an ARM fold ranges from 6 to 12. PMID: 20126549
  8. Data show that p115 degradation plays a key role in amplifying the apoptotic response independently of Golgi fragmentation. PMID: 12438416
  9. p115 acts directly, rather than via a tether, to catalyze trans-SNARE complex formation preceding membrane fusion. PMID: 14736916
  10. The results suggest that p115 is involved in vesicular transport between endoplasmic reticulum and the Golgi, along with microtubule networks. PMID: 16256943
  11. The interaction between p115 and Cog2 was found to be essential for Golgi ribbon reformation after the disruption of the ribbon by p115 KD or brefeldin A treatment and recovery by re-expression of p115 or drug wash out, respectively. PMID: 17274799
  12. Golgi organization depends on mutually interacting domains in betaCOP and p115, suggesting that vesicle tethering at the Golgi involves p115 binding to the COPI coat PMID: 18434597
  13. nuclear import of the p115 CTF is required for it to stimulate the apoptotic response and suggest that its mode of action is confined to the nucleus. PMID: 19028683
  14. terminal repeat folds into the armadillo superhelical groove and allows homodimeric association with important implications for p115 mediated multiple protein interactions and tethering PMID: 19247479
  15. p115 is a critical intermediary component in the regulated secretion of MIF from monocytes/macrophages PMID: 19454686

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Subcellular Location
Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein.
Protein Families
VDP/USO1/EDE1 family
Database Links

HGNC: 30904

OMIM: 603344

KEGG: hsa:8615

UniGene: Hs.744877

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