USO1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA990355A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: A549 whole cell lysate(30µg), HEK293 whole cell lysate(30µg), K562 whole cell lysate(30µg), U-87MG whole cell lysate(30µg), A431 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: USO1 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 108 kDa Observed band size: 108 kDa Exposure time：2min IHC image of CSB-RA990355A0HU diluted at 1:100 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing K562 cells stained with CSB-RA990355A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

O60763

Target Names

USO1

Alternative Names

General vesicular transport factor antibody; General vesicular transport factor p115 antibody; P115 antibody; Protein USO1 homolog antibody; TAP antibody; Transcytosis associated protein antibody; Transcytosis-associated protein antibody; Uso1 antibody; USO1_HUMAN antibody; VDP antibody; Vesicle docking protein antibody; Vesicle docking protein p115 antibody; Vesicle-docking protein antibody

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide from human USO1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

13A9

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

USO1, also known as vesicle-docking protein p115, plays a fundamental role in vesicular transport between the endoplasmic reticulum and Golgi apparatus. This tethering factor facilitates the initial recognition and docking of transport vesicles to target membranes, making it essential for maintaining cellular secretory pathways and protein trafficking. Researchers investigating membrane dynamics, Golgi organization, and intracellular transport mechanisms rely on precise detection of this critical trafficking component.

This recombinant monoclonal antibody offers the consistency and reliability that demanding experimental workflows require. Generated through recombinant technology using a defined clone (13A9), it provides sequence-defined specificity and eliminates the lot-to-lot variability that can compromise longitudinal studies or multi-site collaborations. The rabbit IgG format, raised against a synthetic peptide from human USO1, delivers reproducible results across repeated experiments.

Validation studies demonstrate robust performance across multiple applications and sample types. Western blot analysis confirms detection of the expected 108 kDa band across diverse human cell lines including A549, HEK293, K562, U-87MG, and A431, providing flexibility for various experimental models. Cross-species reactivity extends to mouse and rat brain tissue lysates, supporting translational research using rodent models. Immunohistochemistry validation in paraffin-embedded human gastric cancer tissue demonstrates utility for tissue-based studies, while flow cytometry analysis in K562 cells confirms suitability for single-cell detection approaches.

The broad application compatibility spanning Western blot, immunohistochemistry, flow cytometry, and ELISA makes this antibody a versatile tool for signal transduction research. Whether examining vesicular transport mechanisms, characterizing Golgi function, or investigating protein trafficking in disease contexts, this antibody supports comprehensive experimental approaches with the reproducibility that rigorous research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

General vesicular transport factor required for intercisternal transport in the Golgi stack; it is required for transcytotic fusion and/or subsequent binding of the vesicles to the target membrane. May well act as a vesicular anchor by interacting with the target membrane and holding the vesicular and target membranes in proximity.

Gene References into Functions

USO1 gene may be a promising target for the therapy of human multiple myeloma and its diagnosis marker. PMID: 26898451
USO1 gene has a critical role in human colon cancer: USO1 is involved in cell proliferation and cell migration of colon cancer cells. PMID: 26184508
P115 promotes proliferation of gastric cancer cells through an interaction with MIF. PMID: 24379579
Scyl1 interacts with 58K/formiminotransferase cyclodeaminase (FTCD) and golgin p115, and is required for the maintenance of Golgi morphology. PMID: 20209057
p115 plays a critical role in mitosis progression, implicating it as the only known golgin to regulate both mitosis and apoptosis PMID: 21536679
a conserved tethering function of the Golgi protein p115 CTF which promotes p53-ERK interaction for the amplification of the apoptotic signal. PMID: 21147777
Data show that the number of tandem ARM repeats in an ARM fold ranges from 6 to 12. PMID: 20126549
Data show that p115 degradation plays a key role in amplifying the apoptotic response independently of Golgi fragmentation. PMID: 12438416
p115 acts directly, rather than via a tether, to catalyze trans-SNARE complex formation preceding membrane fusion. PMID: 14736916
The results suggest that p115 is involved in vesicular transport between endoplasmic reticulum and the Golgi, along with microtubule networks. PMID: 16256943
The interaction between p115 and Cog2 was found to be essential for Golgi ribbon reformation after the disruption of the ribbon by p115 KD or brefeldin A treatment and recovery by re-expression of p115 or drug wash out, respectively. PMID: 17274799
Golgi organization depends on mutually interacting domains in betaCOP and p115, suggesting that vesicle tethering at the Golgi involves p115 binding to the COPI coat PMID: 18434597
nuclear import of the p115 CTF is required for it to stimulate the apoptotic response and suggest that its mode of action is confined to the nucleus. PMID: 19028683
terminal repeat folds into the armadillo superhelical groove and allows homodimeric association with important implications for p115 mediated multiple protein interactions and tethering PMID: 19247479
p115 is a critical intermediary component in the regulated secretion of MIF from monocytes/macrophages PMID: 19454686

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Subcellular Location

Cytoplasm, cytosol. Golgi apparatus membrane; Peripheral membrane protein.

Protein Families

VDP/USO1/EDE1 family

Database Links

HGNC: 30904

OMIM: 603344

KEGG: hsa:8615

UniGene: Hs.744877

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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