Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

GGPS1

Uniprot No.

O95749

Research Area

Cancer

Alternative Names

(2E; 6E)-farnesyl diphosphate synthase; Dimethylallyltranstransferase; Farnesyl diphosphate synthase; Farnesyltranstransferase; Geranylgeranyl diphosphate synthase 1; Geranylgeranyl diphosphate synthase; Geranylgeranyl pyrophosphate synthase; Geranylgeranyl pyrophosphate synthetase; Geranyltranstransferase; GGPP synthase; GGPP synthetase; GGPPS; GGPPS_HUMAN; GGPPS1; GGPPSase; GGPS1; OTTHUMP00000036073; OTTHUMP00000216161; OTTHUMP00000216163; OTTHUMP00000216164

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-300aa

Target Protein Sequence

MEKTQETVQRILLEPYKYLLQLPGKQVRTKLSQAFNHWLKVPEDKLQIIIEVTEMLHNASLLIDDIEDNSKLRRGFPVAHSIYGIPSVINSANYVYFLGLEKVLTLDHPDAVKLFTRQLLELHQGQGLDIYWRDNYTCPTEEEYKAMVLQKTGGLFGLAVGLMQLFSDYKEDLKPLLNTLGLFFQIRDDYANLHSKEYSENKSFCEDLTEGKFSFPTIHAIWSRPESTQVQNILRQRTENIDIKKYCVHYLEDVGSFEYTRNTLKELEAKAYKQIDARGGNPELVALVKHLSKMFKEENE
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

50.9kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

This Human GGPS1 recombinant protein was produced in E.coli, where the gene sequence encoding Human GGPS1 (1-300aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this GGPS1 protein was greater than 90% by SDS-PAGE.
Geranylgeranyl pyrophosphate synthase (GGPS1) is a key enzyme in plant terpenoid biosynthesis. The enzyme catalyzes the synthesis of geranylgeranyl pyrophosphate (GGPP), which is a precursor to several holoterpenoids (carotenoids, kaurene, diterpenes) and meroterpenoids (side-chain of chlorophylls, tocopherols, plastoquinones, phylloquinones). The study identified a cDNA for the plastid-located GGPS1 from Capsicum annuum and showed a correlative increase in enzyme activity and transcript level during fruit ripening. The cloned cDNA codes for a high molecular weight precursor of 369 amino acids which contains a transit peptide of approximately 60 amino acids. In-situ immunolocalization experiments have demonstrated that geranylgeranyl pyrophosphate synthase is located exclusively in the plastids.

Reference:
Kuntz M, Römer S, Suire C, Hugueney P, Weil JH, Schantz R, Camara B. Identification of a cDNA for the plastid-located geranylgeranyl pyrophosphate synthase from Capsicum annuum: correlative increase in enzyme activity and transcript level during fruit ripening. Plant J. 1992 Jan;2(1):25-34.

Target Background

Function

Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate, an important precursor of carotenoids and geranylated proteins.

Gene References into Functions

results may support a model in which accumulation of susceptibility variants (including some in relevant genes, notably GGPS1) may lead to a possible genetic component of predisposition to atypical femoral fractures. PMID: 28467865
GGPPS1 may play a critical role during the development of HCC from cirrhosis and is of clinical significance for predicting biological character of HCC. PMID: 24716791
Egr-1 most likely does not induce the constitutive activation of Erk1/2 through its target gene GGPS1. PMID: 23478574
the Egr-1/GGPPS/Erk1/2 pathway is responsible for insulin resistance during hyperinsulinism PMID: 21321112
these results reveal a new EGR-1/GGPPS/MAPK signaling pathway that controls cigarette smoke-induced pulmonary inflammation. PMID: 21224049
Study suggested that GGPS1 -8188A ins/del polymorphism may confer susceptibility to femoral neck BMD response to bisphosphonate therapy in Korean women. PMID: 20191015
crystal structure; it reveals that three dimers join together to form a propeller-bladed hexameric molecule with a mass of approximately 200 kDa PMID: 16698791
Farnesyltranstransferase mutations are associated with lonafarnib resistance PMID: 17536018
results indicate that the active form of GGPS in the solution is an octamer rather than hexamer or dimer PMID: 17646172
Evidence for a non-membrane bound isoform of geranylgeranyl diphosphate synthase 1 in rat. PMID: 8419360

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Subcellular Location

Cytoplasm.

Protein Families

FPP/GGPP synthase family

Tissue Specificity

Abundantly expressed in testis. Found in other tissues to a lower extent.

Database Links

HGNC: 4249

OMIM: 606982

KEGG: hsa:9453

STRING: 9606.ENSP00000282841

UniGene: Hs.730768

Code	CSB-EP009393HU
Abbreviation	Recombinant Human GGPS1 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Geranylgeranyl pyrophosphate synthase (GGPS1)

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