Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
(Glucose-6-phosphatase)(G-6-Pase)(G6Pase)(Glucose-6-phosphatase alpha)(G6Pase-alpha)
Species
Homo sapiens (Human)
Expression Region
82-117aa
Target Protein Sequence
QRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The production process of the recombinant human glucose-6-phosphatase (G6PC) involves fusing a gene fragment encoding amino acids 82 to 117 of the human G6PC protein with a C-terminal 6xHis-tag. This fusion construct is then inserted into a suitable expression vector. Subsequently, the recombinant vector is introduced into the yeast expression system to facilitate the expression of the desired recombinant human G6PC protein. Following successful expression, the recombinant G6PC protein is extracted and purified from the yeast cell lysate. Its purity, determined by SDS-PAGE analysis, is confirmed to be above 85%.
