Recombinant Human Glutaminase liver isoform, mitochondrial(GLS2)

Code CSB-EP887058HU
Size US$2466Purchase it in Cusabio online store
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP887058HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) GLS2.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP887058HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) GLS2.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names GLS2
Uniprot No. Q9UI32
Research Area Metabolism
Alternative Names breast cell glutaminase; GA; GLS; Gls2; GLSL_HUMAN; glutaminase 2 (liver mitochondrial); glutaminase 2; glutaminase GA; glutaminase I; Glutaminase liver isoform; Glutaminase liver isoform; mitochondrial; hLGA; L glutaminase; L glutamine amidohydrolase; L-glutaminase; L-glutamine amidohydrolase; LGA; mitochondrial; phosphate activated glutaminase; phosphate-dependent glutaminase
Species Homo sapiens (Human)
Source E.coli
Expression Region 15-602aa
Target Protein Sequence GSHCGRGGWGHPSRSPLLGGGVRHHLSEAAAQGRETPHSHQPQHQDHDSSESGMLSRLGDLLFYTIAEGQERIPIHKFTTALKATGLQTSDPRLRDCMSEMHRVVQESSSGGLLDRDLFRKCVSSNIVLLTQAFRKKFVIPDFEEFTGHVDRIFEDVKELTGGKVAAYIPQLAKSNPDLWGVSLCTVDGQRHSVGHTKIPFCLQSCVKPLTYAISISTLGTDYVHKFVGKEPSGLRYNKLSLNEEGIPHNPMVNAGAIVVSSLIKMDCNKAEKFDFVLQYLNKMAGNEYMGFSNATFQSEKETGDRNYAIGYYLKEKKCFPKGVDMMAALDLYFQLCSVEVTCESGSVMAATLANGGICPITGESVLSAEAVRNTLSLMHSCGMYDFSGQFAFHVGLPAKSAVSGAILLVVPNVMGMMCLSPPLDKLGNSHRGTSFCQKLVSLFNFHNYDNLRHCARKLDPRREGAEIRNKTVVNLLFAAYSGDVSALRRFALSAMDMEQKDYDSRTALHVAAAEGHIEVVKFLIEACKVNPFAKDRWGNIPLDDAVQFNHLEVVKLLQDYQDSYTLSETQAEAAAEALSKENLESMV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 80.7kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-SUMO-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Gene References into Functions
  1. This study assessed the relation of GLS2 downregulation in glioblastoma cells to its methylation and TP53 status. Aberrant methylation of CpG islands, appear to contribute to silencing of GLS2 in glioblastoma cells by a mechanism bypassing TP53 mutations. PMID: 26258493
  2. observe that while miR-23 is capable of down-regulating the shortened KGA 3'UTR, it has only minor impact on the full-length KGA 3'UTR, demonstrating that additional potent negative regulation of GLS expression exists beyond this single microRNA targeting site PMID: 27095025
  3. the crystal structure of full-length KGA and present a small-angle X-ray scattering model for full-length GLS2. These structures explain these proteins' compromised ability to assemble into catalytically active supra-tetrameric filaments, as previously shown for GAC. PMID: 28526749
  4. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery PMID: 27079415
  5. As a p53 target, GLS2 mediates p53's function in metastasis suppression through inhibiting Rac1. PMID: 26751560
  6. IDO, through GCN2 kinase activation, downregulates the levels of TCRcomplex tchain and cMyc, resulting in the suppression of Tcell proliferation and a reduction in the levels of LDHA and GLS2 PMID: 26647830
  7. GABAergic neurons and astrocytes express Gls and Gls2 isoenzymes in nucleus and mitochondria, in addition to glutamatergic neurons PMID: 25297978
  8. Phosphate-activated glutaminase and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls PMID: 24950944
  9. GLS2 expression is significantly decreased in hepatocellular carcinoma. PMID: 24797434
  10. Silencing GLS or overexpressing GLS2 induces growth inhibition in glioma cell lines. PMID: 24276018
  11. GLS2 is acting, at least in part, downstream of p73 in neuronal differentiation and highlight a possible role of p73 in regulating neurotransmitter synthesis. PMID: 24121663
  12. combination of negative modulation of glutaminase isoforms arising from GLS gene with the introduction of the GLS2 gene product, GAB, may in the future provide a useful means to curb glioblastoma growth in situ PMID: 24096582
  13. Epigenetic silencing of Gls2 via promoter hypermethylation is common in human liver and colon cancers. PMID: 24330717
  14. The present study was to investigate the potent effect of GLS2 on radioresistance of cervical carcinoma. PMID: 23954443
  15. GLS2 is a bona fide TAp63 target gene. PMID: 23574722
  16. LGA circumvents, by an as yet unknown route, the most common mechanism of O6-methylguanine-DNA methyltransferase (MGMT) gene silencing. PMID: 22888977
  17. Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented. PMID: 22679499
  18. GLS2 is resistant to BPTES; GLS1 mutants created reflecting differences between GLS2 and GLS1 sequence in region of BPTES binding; mutant proteins lost inhibition by BPTES; amino acid sequence alignment GLS2 versus GLS1 PMID: 22049910
  19. as a unique p53 target gene, GLS2 is a mediator of p53's role in energy metabolism and antioxidant defense, which can contribute to its role in tumor suppression PMID: 20378837
  20. Phosphate-activated glutaminase (GLS2) is a p53-inducible regulator of glutamine metabolism and reactive oxygen species PMID: 20351271
  21. results show L-type glutaminase is expressed in the brain with a nuclear localization and that this nuclear enzyme is catalytically active and displays kinetic properties different from both liver and kidney glutaminases PMID: 12163477
  22. Data describe the characterization of the human liver-type glutaminase (hLGA) gene. PMID: 12444921
  23. High levels of glutamate were produced by monocyte-derived macrophages infected with HIV-1 strains and inhibited by a glutaminase inhibitor and AZT thus implicating a glutamate-generating enzyme such as phosphate-activated mitochondrial glutaminase PMID: 14675161
  24. K glutaminase isoform is up-regulated with increased rates of proliferation in cancer cells, whereas prevalence of the L isoform seems to be related with resting or quiescent cell states PMID: 15496140
  25. These data implicate mitochondrial glutaminase in the induction of glutamate-mediated neuronal apoptosis during HIV-associated dementia, and provides a possible therapeutic strategy for HAD treatment. PMID: 18088378
  26. presence of LGA mRNA in transfected glioma cells coincided with depression of basic physiological parameters: Cell migration, proliferation, and survival as assessed with colony formation assay and the mitochondrial activity test PMID: 19062176

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Subcellular Location Mitochondrion.
Protein Families Glutaminase family
Tissue Specificity Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Database Links

HGNC: 29570

OMIM: 606365

KEGG: hsa:27165

STRING: 9606.ENSP00000310447

UniGene: Hs.212606

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