GLS2 Antibody

Code CSB-PA887058LA01HU
Size US$299 How to order?
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  • Immunofluorescence staining of HepG2 cells with CSB-PA887058LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) GLS2 Polyclonal antibody
Uniprot No. Q9UI32
Target Names GLS2
Alternative Names breast cell glutaminase antibody; GA antibody; GLS antibody; Gls2 antibody; GLSL_HUMAN antibody; glutaminase 2 (liver mitochondrial) antibody; glutaminase 2 antibody; glutaminase GA antibody; glutaminase I antibody; Glutaminase liver isoform antibody; Glutaminase liver isoform; mitochondrial antibody; hLGA antibody; L glutaminase antibody; L glutamine amidohydrolase antibody; L-glutaminase antibody; L-glutamine amidohydrolase antibody; LGA antibody; mitochondrial antibody; phosphate activated glutaminase antibody; phosphate-dependent glutaminase antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Glutaminase liver isoform, mitochondrial protein (15-602AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated

The GLS2 Antibody (Product code: CSB-PA887058LA01HU) is Non-conjugated. For GLS2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA887058LB01HU GLS2 Antibody, HRP conjugated ELISA
FITC CSB-PA887058LC01HU GLS2 Antibody, FITC conjugated
Biotin CSB-PA887058LD01HU GLS2 Antibody, Biotin conjugated ELISA
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, IF
Recommended Dilution
Application Recommended Dilution
IF 1:50-1:200
Protocols ELISA Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.
Gene References into Functions
  1. This study assessed the relation of GLS2 downregulation in glioblastoma cells to its methylation and TP53 status. Aberrant methylation of CpG islands, appear to contribute to silencing of GLS2 in glioblastoma cells by a mechanism bypassing TP53 mutations. PMID: 26258493
  2. observe that while miR-23 is capable of down-regulating the shortened KGA 3'UTR, it has only minor impact on the full-length KGA 3'UTR, demonstrating that additional potent negative regulation of GLS expression exists beyond this single microRNA targeting site PMID: 27095025
  3. the crystal structure of full-length KGA and present a small-angle X-ray scattering model for full-length GLS2. These structures explain these proteins' compromised ability to assemble into catalytically active supra-tetrameric filaments, as previously shown for GAC. PMID: 28526749
  4. Cox multivariate regression analysis demonstrated that DUOX1, GLS2, FBP1 and age were independent risk factors for the prognosis of HCC patients after surgery PMID: 27079415
  5. As a p53 target, GLS2 mediates p53's function in metastasis suppression through inhibiting Rac1. PMID: 26751560
  6. IDO, through GCN2 kinase activation, downregulates the levels of TCRcomplex tchain and cMyc, resulting in the suppression of Tcell proliferation and a reduction in the levels of LDHA and GLS2 PMID: 26647830
  7. GABAergic neurons and astrocytes express Gls and Gls2 isoenzymes in nucleus and mitochondria, in addition to glutamatergic neurons PMID: 25297978
  8. Phosphate-activated glutaminase and GAD65/67 concentrations are compared in Alzheimer's disease cerebellum versus normal cerebellum controls PMID: 24950944
  9. GLS2 expression is significantly decreased in hepatocellular carcinoma. PMID: 24797434
  10. Silencing GLS or overexpressing GLS2 induces growth inhibition in glioma cell lines. PMID: 24276018
  11. GLS2 is acting, at least in part, downstream of p73 in neuronal differentiation and highlight a possible role of p73 in regulating neurotransmitter synthesis. PMID: 24121663
  12. combination of negative modulation of glutaminase isoforms arising from GLS gene with the introduction of the GLS2 gene product, GAB, may in the future provide a useful means to curb glioblastoma growth in situ PMID: 24096582
  13. Epigenetic silencing of Gls2 via promoter hypermethylation is common in human liver and colon cancers. PMID: 24330717
  14. The present study was to investigate the potent effect of GLS2 on radioresistance of cervical carcinoma. PMID: 23954443
  15. GLS2 is a bona fide TAp63 target gene. PMID: 23574722
  16. LGA circumvents, by an as yet unknown route, the most common mechanism of O6-methylguanine-DNA methyltransferase (MGMT) gene silencing. PMID: 22888977
  17. Study demonstrated expression of alternative transcripts of the mammalian Gls2 gene. Transcriptional mechanisms giving rise to GLS2 variants and isolation of novel GLS2 transcripts in human, rat and mouse are presented. PMID: 22679499
  18. GLS2 is resistant to BPTES; GLS1 mutants created reflecting differences between GLS2 and GLS1 sequence in region of BPTES binding; mutant proteins lost inhibition by BPTES; amino acid sequence alignment GLS2 versus GLS1 PMID: 22049910
  19. as a unique p53 target gene, GLS2 is a mediator of p53's role in energy metabolism and antioxidant defense, which can contribute to its role in tumor suppression PMID: 20378837
  20. Phosphate-activated glutaminase (GLS2) is a p53-inducible regulator of glutamine metabolism and reactive oxygen species PMID: 20351271
  21. results show L-type glutaminase is expressed in the brain with a nuclear localization and that this nuclear enzyme is catalytically active and displays kinetic properties different from both liver and kidney glutaminases PMID: 12163477
  22. Data describe the characterization of the human liver-type glutaminase (hLGA) gene. PMID: 12444921
  23. High levels of glutamate were produced by monocyte-derived macrophages infected with HIV-1 strains and inhibited by a glutaminase inhibitor and AZT thus implicating a glutamate-generating enzyme such as phosphate-activated mitochondrial glutaminase PMID: 14675161
  24. K glutaminase isoform is up-regulated with increased rates of proliferation in cancer cells, whereas prevalence of the L isoform seems to be related with resting or quiescent cell states PMID: 15496140
  25. These data implicate mitochondrial glutaminase in the induction of glutamate-mediated neuronal apoptosis during HIV-associated dementia, and provides a possible therapeutic strategy for HAD treatment. PMID: 18088378
  26. presence of LGA mRNA in transfected glioma cells coincided with depression of basic physiological parameters: Cell migration, proliferation, and survival as assessed with colony formation assay and the mitochondrial activity test PMID: 19062176

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Subcellular Location Mitochondrion.
Protein Families Glutaminase family
Tissue Specificity Highly expressed in liver. Expressed in brain and pancreas. Not observed in heart, placenta, lung, skeletal muscle and kidney. Expression is significantly reduced in hepatocellular carcinomas.
Database Links

HGNC: 29570

OMIM: 606365

KEGG: hsa:27165

STRING: 9606.ENSP00000310447

UniGene: Hs.212606

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