Recombinant Human Golgi reassembly-stacking protein 2 (GORASP2)

Code CSB-YP880999HU
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Source Yeast
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Code CSB-EP880999HU
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Source E.coli
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Code CSB-EP880999HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880999HU
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Source Baculovirus
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Code CSB-MP880999HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GORASP2
Uniprot No.
Alternative Names
AW552058; DKFZp434D156; FLJ13139; Golgi phosphoprotein 6; Golgi reassembly stacking protein 2; Golgi reassembly stacking protein 2; 55kDa; Golgi reassembly stacking protein of 55 kDa; Golgi reassembly-stacking protein 2; Golgi reassembly-stacking protein of 55 kDa; GOLPH2; GOLPH6; GORASP2; GORS2_HUMAN; GRASP55; GRS2; p59; RP23-428O15.1
Species
Homo sapiens (Human)
Expression Region
2-452
Target Protein Sequence
GSSQSVEIP GGGTEGYHVL RVQENSPGHR AGLEPFFDFI VSINGSRLNK DNDTLKDLLK ANVEKPVKML IYSSKTLELR ETSVTPSNLW GGQGLLGVSI RFCSFDGANE NVWHVLEVES NSPAALAGLR PHSDYIIGAD TVMNESEDLF SLIETHEAKP LKLYVYNTDT DNCREVIITP NSAWGGEGSL GCGIGYGYLH RIPTRPFEEG KKISLPGQMA GTPITPLKDG FTEVQLSSVN PPSLSPPGTT GIEQSLTGLS ISSTPPAVSS VLSTGVPTVP LLPPQVNQSL TSVPPMNPAT TLPGLMPLPA GLPNLPNLNL NLPAPHIMPG VGLPELVNPG LPPLPSMPPR NLPGIAPLPL PSEFLPSFPL VPESSSAASS GELLSSLPPT SNAPSDPATT TAKADAASSL TVDVTPPTAK APTTVEDRVG DSTPVSEKPV SAAVDANASE SP
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after breakdown during mitosis and meiosis. May regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA. Required for normal acrosome formation during spermiogenesis and normal male fertility, probably by promoting colocalization of JAM2 and JAM3 at contact sites between germ cells and Sertoli cells. Mediates ER stress-induced unconventional (ER/Golgi-independent) trafficking of core-glycosylated CFTR to cell membrane.
Gene References into Functions
  1. Results demonstrate a critical role for GRASP55 and GRASP65 in maintaining the stacked structure of the Golgi, which is required for accurate posttranslational modifications in the Golgi. Additionally, the GRASP knockout cell lines developed in this study will be useful tools for studying the role of GRASP proteins in other important cellular processes. PMID: 28814501
  2. binding site at the cleft between the PDZ1 and PDZ2 domains of GRASP65 is dominated by hydrophobic interactions with GM130 that are not observed in the GRASP55-Golgin45 complex PMID: 28049725
  3. GRASP55 interacts with CD83 shortly after induction of dendritic cells maturation. PMID: 25701785
  4. Cisternal-specific functions of GRASP65 and GRASP55 in continuity, compartmentalization, and function of the Golgi ribbon. PMID: 24227884
  5. propose that GRASP55/65 are negative regulators of exocytic transport and that this slowdown helps to ensure more complete protein glycosylation in the Golgi stack and proper sorting at the trans-Golgi network PMID: 23552074
  6. GRASP55 may function as an adaptor protein coupling MT1-MMP with furin, thus leading to the activation of the zymogen. PMID: 20608975
  7. These results demonstrate that GRASP55 and GRASP65 stack mammalian Golgi cisternae via a common mechanism. PMID: 20083603
  8. Data demonstrate that both GRASP55 and 65 are needed for the efficient transport to and through the Golgi complex, thus highlighting a novel role for the GRASPs in membrane trafficking. PMID: 19840934
  9. GRASP65 is an important structural component required for maintenance of Golgi apparatus integrity PMID: 11815631
  10. MEK1/ERK regulation of GRASP55-mediated Golgi linking is a control point in cell cycle progression PMID: 18434598
  11. GRASP55 forms an effector complex for the small GTPase rab2, together with a conserved coiled-coil protein golgin-45 (JEM-1). PMID: 11739401

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Subcellular Location
Golgi apparatus membrane; Lipid-anchor. Endoplasmic reticulum membrane. Golgi apparatus.
Protein Families
GORASP family
Database Links

HGNC: 17500

OMIM: 608693

KEGG: hsa:26003

STRING: 9606.ENSP00000234160

UniGene: Hs.431317

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