Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ERVK-6

Uniprot No.

Q69384

Research Area

others

Alternative Names

ERVK-6; ERVK6Endogenous retrovirus group K member 6 Env polyprotein; EnvK2 protein; Envelope polyprotein; HERV-K(C7) envelope protein; HERV-K(HML-2.HOM) envelope protein; HERV-K108 envelope protein; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein; SU); Transmembrane protein; TM)]

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

90-632aa

Target Protein Sequence

LPMPAGAAAANYTYWAYVPFPPLIRAVTWMDNPTEVYVNDSVWVPGPIDDRCPAKPEEEGMMINISIGYHYPPICLGRAPGCLMPAVQNWLVEVPTVSPICRFTYHMVSGMSLRPRVNYLQDFSYQRSLKFRPKGKPCPKEIPKESKNTEVLVWEECVANSAVILQNNEFGTIIDWAPRGQFYHNCSGQTQSCPSAQVSPAVDSDLTESLDKHKHKKLQSFYPWEWGEKGISTPRPKIVSPVSGPEHPELWRLTVASHHIRIWSGNQTLETRDRKPFYTIDLNSSLTVPLQSCVKPPYMLVVGNIVIKPDSQTITCENCRLLTCIDSTFNWQHRILLVRAREGVWIPVSMDRPWEASPSVHILTEVLKGVLNRSKRFIFTLIAVIMGLIAVTATAAVAGVALHSSVQSVNFVNDWQKNSTRLWNSQSSIDQKLANQINDLRQTVIWMGDRLMSLEHRFQLQCDWNTSDFCITPQIYNESEHHWDMVRRHLQGREDNLTLDISKLKEQIFEASKAHLNLVPGTEAIAGVADGLANLNPVTWVKT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

65.5 kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

N-terminal 6xHis-tagged recombinant human Endogenous retrovirus group K member 6 Env polyprotein (ERVK-6) is a partial-length protein containing amino acid 90-632. It is the extracellular domain of human ERVK-6 protein and can be used for the production of corresponding specific antibodies. Procaryotic expression (E.coli) confers this recombinant ERVK-6 good features such as clear genetic background and relatively simple separation ＆ purification. The purity of ERVK-6 protein is greater than 85% determined by SDS-PAGE. This recombinant ERVK-6 protein may also be used in the studies of microbiology.

Human ERVK-6 is a member of human endogenous retrovirus type K (HERV-K), the sole group of endogenous retroviruses that are established to contain human-specific members. As a retroviral envelope protein, ERVK-6 mediates receptor recognition and membrane fusion during early infection. This endogenous envelope protein has lost its original fusogenic properties during evolution.

Customer Reviews and Q&A

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■ Q&A

What is the highest recommended stock concentration for reconstitution? I see the info suggests 1mg/ml. Can other liquids such as PBS or medium be used to reconstitute or will this affect the solubility?

Reconstitution instructions:
The lyophilized protein can be reconstituted with deionized sterile water.
Briefly centrifuge lyophilized product prior to opening to bring the contents to the bottom, and then reconstitute the protein to 0.1-1.0 mg/mL by adding deionized sterile water.
If a large amount of dilution is needed, it needs to be diluted with corresponding Tris buffer.
If you prefer PBS buffer, we recommend you try small amount first to see if it is stable.
As you mentioned medium, will you use this protein for cell experiment?
If yes, we haven't remove the endotonxin from the inventory, we need prepare a new lot and Endotoxin Removal Service and l Aseptic Manufacture Processing free of charge. Lead time is 18-28 working days.
We use conjugated PMB affinity filler adsorption to remove endotoxin and guarantee endotoxin level within 0.1ng/ug (1EU/ug).

Target Background

Function

Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.

Subcellular Location

[Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.

Protein Families

Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily

Tissue Specificity

Expressed in testis, and peripheral blood lymphocytes.

Database Links

HGNC: 13915

OMIM: 605626

Code	CSB-EP724336HUa0
Abbreviation	Recombinant Human ERVK-6 protein, partial
MSDS	MSDS Datasheet
Size	US$306
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Recombinant Human Endogenous retrovirus group K member 6 Env polyprotein (ERVK-6), partial

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