Recombinant Human Heart- and neural crest derivatives-expressed protein 1 (HAND1)

Code CSB-YP010125HU
MSDS
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Source Yeast
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Code CSB-EP010125HU
MSDS
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Source E.coli
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Code CSB-EP010125HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010125HU
MSDS
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Source Baculovirus
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Code CSB-MP010125HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HAND1
Uniprot No.
Alternative Names
autonomic nervous system and neural crest derivatives-expressed protein 1; Basic helix loop helix transcription factor HAND1; bHLHa27; Class A basic helix-loop-helix protein 27; eHAND; Extraembryonic tissues; Extraembryonic tissues heart autonomic nervous system and neural crest derivatives expressed protein 1; HAND 1; HAND1; HAND1_HUMAN; Heart and neural crest derivatives expressed 1; Heart and neural crest derivatives expressed protein 1; heart; Heart- and neural crest derivatives-expressed protein 1; Hxt; Thing 1; Thing1 ; Thing1
Species
Homo sapiens (Human)
Expression Region
1-215
Target Protein Sequence
MNLVGSYAHH HHHHHPHPAH PMLHEPFLFG PASRCHQERP YFQSWLLSPA DAAPDFPAGG PPPAAAAAAT AYGPDARPGQ SPGRLEALGG RLGRRKGSGP KKERRRTESI NSAFAELREC IPNVPADTKL SKIKTLRLAT SYIAYLMDVL AKDAQSGDPE AFKAELKKAD GGRESKRKRE LQQHEGFPPA LGPVEKRIKG RTGWPQQVWA LELNQ
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that plays an essential role in both trophoblast giant cell differentiation and in cardiac morphogenesis. Binds the DNA sequence 5'-NRTCTG-3' (non-canonical E-box). Acts as a transcriptional repressor of SOX15. In the adult, could be required for ongoing expression of cardiac-specific genes.
Gene References into Functions
  1. Strikingly, human pluripotent stem cell antibody array showed that Hand1 overexpression resulted in substantial decrease in pluripotency markers (Nanog, Oct3/4, Otx2, Flk1) suggesting that Hand1 expression may be essential to attenuate the EMT and our findings underscore a novel role for Hand1 in medulloblastoma metastasis. PMID: 27297109
  2. association of HAND1 loss-of-function mutation with increased susceptibility to Tetralogy of Fallot PMID: 27942761
  3. These findings expand the phenotypic spectrum linked to HAND1 mutations, suggesting potential implications for the development of novelo prophylactic and therapeutic strategies for DORV. PMID: 28112363
  4. HAND1 loss-of-function mutation is associated with familial dilated cardiomyopathy. PMID: 26581070
  5. Combined expression of NKX2-5, HAND1, and NOTCH1 coordinately contribute to cardiac malformations in Hhypoplastic left heart syndrome. PMID: 25050861
  6. These data show that miR-363 negatively regulates the expression of HAND1 PMID: 24906886
  7. Nuclear translocation of Hand-1 acts as a molecular switch to regulate vascular radiosensitivity in medulloblastoma tumors. PMID: 24623737
  8. DNA methylation status of NKX2-5, GATA4 and HAND1 in patients with tetralogy of fallot PMID: 24182332
  9. Increased methylation levels of HAND1 is associated with highly active Helicobacter pylori-related gastritis. PMID: 23292007
  10. This is the first report of mutations in the HAND1 gene in Chinese patients with VSD and provides new insight into the etiology of VSD PMID: 22032825
  11. Hand1 is dispensable for normal tyrosine hydroxylase and dopamine beta-hydroxylase expression in sympathetic neurons, even when Hand2 gene dosage is concurrently reduced by half. PMID: 22323723
  12. Somatic mutations in NKX2-5, GATA4, and HAND1 are not a common cause of tetralogy of Fallot or hypoplastic left heart. PMID: 22043484
  13. Mutations or sequence variations in HAND1 or NKX2-5 genes may play role in etiology or pathogenesis of atrial isomerism. PMID: 21561848
  14. Our study shows no evidence of somatic NKX2-5, GATA4 and HAND1 mutations playing a role in the pathogenesis of Tetralogy of Fallot . Findings suggest that the GATA4 and HAND1 germline mutations are associated with non-syndromic congenital heart disease. PMID: 21519287
  15. Hand1 lineage marks the proepicardial organ and epicardium necessary for epicardial and coronary vessel development, defining the epicardial precursors that are subsequently dependent on Hand2 function. PMID: 21350214
  16. effects of gene mutations on ventricular development PMID: 12858532
  17. MEF2/HAND1 interaction results in synergistic activation of MEF2-dependent promoters, and MEF2 binding sites are sufficient to mediate this synergy PMID: 16043483
  18. In 24 of 31 hypoplastic ventricles, a frameshift mutation was detected in the bHLH domain, which is necessary for DNA binding and combinatorial interactions; thus in hypoplastic human hearts HAND1 function is impaired. PMID: 18276607
  19. HMGA1 proteins bind directly to Hand1 promoter both in vitro and in vivo and inhibit Hand1 promoter activity PMID: 19060921
  20. HAND1 sequence mutations are frequent in human hearts with septation defects. PMID: 19586923

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Subcellular Location
Nucleus, nucleoplasm. Nucleus, nucleolus.
Tissue Specificity
Heart.
Database Links

HGNC: 4807

OMIM: 602406

KEGG: hsa:9421

STRING: 9606.ENSP00000231121

UniGene: Hs.152531

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